Anti-CD22 antibody [BLCAM/1795] (ab218340)
Key features and details
- Mouse monoclonal [BLCAM/1795] to CD22
- Suitable for: Protein Array, WB, IHC-P, Flow Cyt, ICC
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-CD22 antibody [BLCAM/1795]
See all CD22 primary antibodies -
Description
Mouse monoclonal [BLCAM/1795] to CD22 -
Host species
Mouse -
Tested applications
Suitable for: Protein Array, WB, IHC-P, Flow Cyt, ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human CD22 aa 52-178. The exact sequence is proprietary.
Database link: P20273 -
Positive control
- WB: Raji and Ramos cell lysates; IHC-P: Human tonsil tissue; Flow Cyt: MOLT-4 cells; ICC: Ramos cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, 99% PBS -
Concentration information loading...
-
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
BLCAM/1795 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab218340 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Protein Array |
Use at an assay dependent concentration.
|
|
WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 95 kDa.
|
|
IHC-P |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
Flow Cyt |
Use 1-2µg for 106 cells.
|
|
ICC |
Use a concentration of 1 - 2 µg/ml.
|
Notes |
---|
Protein Array
Use at an assay dependent concentration. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 95 kDa. |
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Flow Cyt
Use 1-2µg for 106 cells. |
ICC
Use a concentration of 1 - 2 µg/ml. |
Target
-
Function
Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules. -
Tissue specificity
B-lymphocytes. -
Sequence similarities
Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Domain
Contains 4 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. -
Post-translational
modificationsPhosphorylation of Tyr-762, Tyr-807 and Tyr-822 are involved in binding to SYK, GRB2 and SYK, respectively. Phosphorylation of Tyr-842 is involved in binding to SYK, PLCG2 and PIK3R1/PIK3R2.
Phosphorylated on tyrosine residues by LYN. -
Cellular localization
Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 933 Human
- Omim: 107266 Human
- SwissProt: P20273 Human
- Unigene: 579691 Human
-
Alternative names
- B cell receptor CD22 precursor antibody
- B lymphocyte cell adhesion molecule antibody
- B-cell receptor CD22 antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling CD22 with ab218340.
-
Immunocytochemistry analysis of paraformaldehyde-fixed Ramos (human Burkitt's lymphoma cell line) cells labeling CD22 with ab218340 followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.
-
Flow Cytometry analysis of paraformaldehyde-fixed MOLT-4 (human lymphoblastic leukemia cell line) cells labeling CD22 with ab218340 followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
-
All lanes : Anti-CD22 antibody [BLCAM/1795] (ab218340) at 1 µg/ml
Lane 1 : Raji cell lysate
Lane 2 : Ramos cell lysate
Predicted band size: 95 kDa -
Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human tonsil tissue labeling CD22 with ab218340 at 2 μg/ml dilution, followed by conjugation to the secondary antibody.
-
ab218340 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab218340 has been referenced in 1 publication.
- Schloesser D et al. Senescent cells suppress macrophage-mediated corpse removal via upregulation of the CD47-QPCT/L axis. J Cell Biol 222:N/A (2023). PubMed: 36459066