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RecombinantRabMAb

Recombinant Anti-CD22 antibody [EP498Y] - BSA and Azide free (ab247276)

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Western blot - Anti-CD22 antibody [EP498Y] - BSA and Azide free (ab247276)
  • Western blot - Anti-CD22 antibody [EP498Y] - BSA and Azide free (ab247276)
  • Western blot - Anti-CD22 antibody [EP498Y] - BSA and Azide free (ab247276)
  • Anti-CD22 antibody [EP498Y] - BSA and Azide free (ab247276)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP498Y] to CD22 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

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Conjugation
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HRP Conjugation Kit - Lightning-Link® (ab102890)

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Overview

  • Product name

    Anti-CD22 antibody [EP498Y] - BSA and Azide free
    See all CD22 primary antibodies
  • Description

    Rabbit monoclonal [EP498Y] to CD22 - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    This antibody recognises CD22. 

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CD22 aa 1-100 (N terminal). The exact sequence is proprietary.

  • General notes

    Ab247276 is the carrier-free version of ab33859. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab247276 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP498Y
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • B Cells
    • CD
    • Cancer
    • Tumor immunology
    • Tumor-associated antigens
    • Cancer
    • Tumor biomarkers
    • Tumor antigens
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • B Lymphocytic Lineage

Associated products

  • Alternative Versions

    • Anti-CD22 antibody [EP498Y] (ab33859)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
  • Positive Controls

    • Raji whole cell lysate (ab30124)

Applications

Our Abpromise guarantee covers the use of ab247276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 150 kDa (predicted molecular weight: 95 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.
    • Tissue specificity

      B-lymphocytes.
    • Sequence similarities

      Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
      Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Domain

      Contains 4 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
    • Post-translational
      modifications

      Phosphorylation of Tyr-762, Tyr-807 and Tyr-822 are involved in binding to SYK, GRB2 and SYK, respectively. Phosphorylation of Tyr-842 is involved in binding to SYK, PLCG2 and PIK3R1/PIK3R2.
      Phosphorylated on tyrosine residues by LYN.
    • Cellular localization

      Cell membrane.
    • Target information above from: UniProt accession P20273 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 933 Human
      • Entrez Gene: 12483 Mouse
      • Entrez Gene: 308501 Rat
      • Omim: 107266 Human
      • SwissProt: P20273 Human
      • SwissProt: P35329 Mouse
      • Unigene: 579691 Human
      • Unigene: 260994 Mouse
      • Alternative names

        • B cell receptor CD22 precursor antibody
        • B lymphocyte cell adhesion molecule antibody
        • B-cell receptor CD22 antibody
        • B-lymphocyte cell adhesion molecule antibody
        • BL CAM antibody
        • BL-CAM antibody
        • BLCAM antibody
        • CD 22 antibody
        • CD22 antibody
        • CD22 antigen antibody
        • CD22 molecule antibody
        • CD22 protein antibody
        • CD22_HUMAN antibody
        • Lectin 2 antibody
        • Leu14 antibody
        • Lyb8 antibody
        • MGC130020 antibody
        • sialic acid binding Ig like lectin 2 antibody
        • Sialic acid binding immunoglobulin like lectin 2 antibody
        • Sialic acid-binding Ig-like lectin 2 antibody
        • SIGLEC 2 antibody
        • Siglec-2 antibody
        • SIGLEC2 antibody
        • T cell surface antigen Leu 14 antibody
        • T-cell surface antigen Leu-14 antibody
        see all

      Images

      • Western blot - Anti-CD22 antibody [EP498Y] - BSA and Azide free (ab247276)
        Western blot - Anti-CD22 antibody [EP498Y] - BSA and Azide free (ab247276)
        All lanes : Anti-CD22 antibody [EP498Y] (ab33859) at 1/1000 dilution (purified)

        Lane 1 : Rat spleen tissue lysate
        Lane 2 : RAW 264.7 whole cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

        Predicted band size: 95 kDa
        Observed band size: 150 kDa
        why is the actual band size different from the predicted?



        This data was developed using ab33859, the same antibody clone in a different buffer formulation.

        Blocking and dilution buffer: 5% NFDM/TBST.

      • Western blot - Anti-CD22 antibody [EP498Y] - BSA and Azide free (ab247276)
        Western blot - Anti-CD22 antibody [EP498Y] - BSA and Azide free (ab247276)
        Anti-CD22 antibody [EP498Y] (ab33859) at 1/5000 dilution (purified) + Raji whole cell lysate at 10 µg

        Secondary
        Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

        Predicted band size: 95 kDa
        Observed band size: 150 kDa why is the actual band size different from the predicted?



        This data was developed using ab33859, the same antibody clone in a different buffer formulation.

        Blocking and dilution buffer: 5% NFDM/TBST.

      • Western blot - Anti-CD22 antibody [EP498Y] - BSA and Azide free (ab247276)
        Western blot - Anti-CD22 antibody [EP498Y] - BSA and Azide free (ab247276)
        Anti-CD22 antibody [EP498Y] (ab33859) at 1/1000 dilution (unpurified) + Raji cell lysate

        Predicted band size: 95 kDa
        Observed band size: 150 kDa why is the actual band size different from the predicted?



        This data was developed using ab33859, the same antibody clone in a different buffer formulation.

         

      • Anti-CD22 antibody [EP498Y] - BSA and Azide free (ab247276)
        Anti-CD22 antibody [EP498Y] - BSA and Azide free (ab247276)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet

      Certificate of Compliance

      To download a Certificate of Compliance, please enter your Lot number below:

    • References (0)

      Publishing research using ab247276? Please let us know so that we can cite the reference in this datasheet.

      ab247276 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      There are currently no Customer reviews or Questions for ab247276.
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