Key features and details
- Mouse monoclonal [RFB4] to CD22 (PE)
- Suitable for: IHC-Fr, Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Product nameAnti-CD22 antibody [RFB4] (PE)
See all CD22 primary antibodies
DescriptionMouse monoclonal [RFB4] to CD22 (PE)
ConjugationPE. Ex: 488nm, Em: 575nm
SpecificityCD22 is in principle a B lineage antigen, that is present in the cytoplasm of progenitor B-cells and on the membrane of the majority of mature peripheral B-lymphocytes. Furthermore, it is strongly expressed on Hairy Cell Leukemia cells and very weakly on some other leukemias. Antigen distribution: Peripheral blood lymphocytes 12±3% T-cells (E+) < 1% B-cells (E-, Ig+) 92±3% Monocytes (CD14+) < 1% Granulocytes < 1% Thymocytes < 1%
Tested applicationsSuitable for: IHC-Fr, Flow Cytmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Human
Purified tonsil lymphocytes (Human).
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferPreservative: 0.1% Sodium azide
Constituent: 1% BSA
Concentration information loading...
Purification notesPurification by IEP (immunoelectrophoresis).
Primary antibody notesab23620 can be used for: - Quantitative determination of peripheral B-cells (CD22+) in blood. - Identification of CD22+-cells in tissue sections. - Determination of B-cell origin of lymphoid neoplasms. - Quantitative elimination or isolation of B-cells by flow cytometry or magnet beads.
Our Abpromise guarantee covers the use of ab23620 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|Flow Cyt||Use 5µl for 106 cells.
Use maximum of 5µl for 106 cells.
ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionMediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.
Sequence similaritiesBelongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
DomainContains 4 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
modificationsPhosphorylation of Tyr-762, Tyr-807 and Tyr-822 are involved in binding to SYK, GRB2 and SYK, respectively. Phosphorylation of Tyr-842 is involved in binding to SYK, PLCG2 and PIK3R1/PIK3R2.
Phosphorylated on tyrosine residues by LYN.
Cellular localizationCell membrane.
- Information by UniProt
- B cell receptor CD22 precursor antibody
- B lymphocyte cell adhesion molecule antibody
- B-cell receptor CD22 antibody
ab23620 has been referenced in 2 publications.
- Ito D et al. A double blinded, placebo-controlled pilot study to examine reduction of CD34 +/CD117 +/CD133 + lymphoma progenitor cells and duration of remission induced by neoadjuvant valspodar in dogs with large B-cell lymphoma. F1000Res 4:42 (2015). PubMed: 28357033
- Ito D et al. Exclusion of cytoplasmic fragments in flow cytometric analysis of lymph node samples from dogs with lymphoma using membrane-permeable violet laser-excitable DNA-binding fluorescent dye (DyeCycle Violet). Vet Clin Pathol 39:494-8 (2010). PubMed: 21198735