Recombinant
RabMAb

Recombinant Anti-CD22 antibody [SP104] - C-terminal (ab227645)

Rabbit recombinant monoclonal CD22 antibody [SP104]. Validated in IHC and tested in Human.

Overview

  • Product name

    Anti-CD22 antibody [SP104] - C-terminal
    See all CD22 primary antibodies
  • Description

    Rabbit monoclonal [SP104] to CD22 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Dog
  • Immunogen

    Synthetic peptide within Human CD22 aa 250-350 (extracellular). The exact sequence is proprietary.
    Database link: P20273

  • Positive control

    • IHC-P: Human tonsil, spleen, reactive lymph node and HK lymphoma tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab227645 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Primary antibody incubation for 30 minutes at room temperature.

Target

  • Function

    Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.
  • Tissue specificity

    B-lymphocytes.
  • Sequence similarities

    Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
    Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Domain

    Contains 4 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
  • Post-translational
    modifications

    Phosphorylation of Tyr-762, Tyr-807 and Tyr-822 are involved in binding to SYK, GRB2 and SYK, respectively. Phosphorylation of Tyr-842 is involved in binding to SYK, PLCG2 and PIK3R1/PIK3R2.
    Phosphorylated on tyrosine residues by LYN.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • B cell receptor CD22 precursor antibody
    • B lymphocyte cell adhesion molecule antibody
    • B-cell receptor CD22 antibody
    • B-lymphocyte cell adhesion molecule antibody
    • BL CAM antibody
    • BL-CAM antibody
    • BLCAM antibody
    • CD 22 antibody
    • CD22 antibody
    • CD22 antigen antibody
    • CD22 molecule antibody
    • CD22 protein antibody
    • CD22_HUMAN antibody
    • Lectin 2 antibody
    • Leu14 antibody
    • Lyb8 antibody
    • MGC130020 antibody
    • sialic acid binding Ig like lectin 2 antibody
    • Sialic acid binding immunoglobulin like lectin 2 antibody
    • Sialic acid-binding Ig-like lectin 2 antibody
    • SIGLEC 2 antibody
    • Siglec-2 antibody
    • SIGLEC2 antibody
    • T cell surface antigen Leu 14 antibody
    • T-cell surface antigen Leu-14 antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD22 using ab227645 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human spleen tissue stained for CD22 using ab227645 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD22 using ab227645 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human reactive lymph node tissue stained for CD22 using ab227645 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human HK lymphoma tissue stained for CD22 using ab227645 at 1/100 dilution in immunohistochemical analysis.

References

ab227645 has not yet been referenced specifically in any publications.

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