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Immunology Adaptive Immunity B Cells CD
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RabMAb

Anti-CD22 (phospho Y822) antibody [Y506] (ab32123)

  • Datasheet
Reviews (1) Submit a question References (2)

Product price, shipping and contact information

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Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Flow Cytometry - Anti-CD22 (phospho Y822) antibody [Y506] (ab32123)
  • Western blot - Anti-CD22 (phospho Y822) antibody [Y506] (ab32123)
  • Dot Blot - Anti-CD22 (phospho Y822) antibody [Y506] (ab32123)
  • Western blot - Anti-CD22 (phospho Y822) antibody [Y506] (ab32123)

Key features and details

  • Rabbit monoclonal [Y506] to CD22 (phospho Y822)
  • Suitable for: WB, Flow Cyt, Dot blot
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-CD22 (phospho Y822) antibody [Y506]
    See all CD22 primary antibodies
  • Description

    Rabbit monoclonal [Y506] to CD22 (phospho Y822)
  • Host species

    Rabbit
  • Specificity

    ab32123 recognises CD22 phosphorylation on Tyrosine 822. This antibody is predicted to detect splice isoform 2 based on sequence homology.
  • Tested applications

    Suitable for: WB, Flow Cyt, Dot blotmore details
    Unsuitable for: IHC or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    A synthetic phospho-peptide corresponding to residues surrounding Tyrosine 822 of human CD22

  • Positive control

    • WB: Ramos and Raji cell lysates treated with Pervanadate. Flow Cyt: K562 cells treated with Pervanadate.
  • General notes

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    IgG fraction
  • Clonality

    Monoclonal
  • Clone number

    Y506
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • B Cells
    • CD
    • Cancer
    • Tumor immunology
    • Tumor-associated antigens
    • Cancer
    • Tumor biomarkers
    • Tumor antigens
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • B Lymphocytic Lineage

Associated products

  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

Our Abpromise guarantee covers the use of ab32123 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 150 kDa (predicted molecular weight: 95 kDa).
Flow Cyt 1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Dot blot 1/1000.
  • Application notes
    Is unsuitable for IHC or IP.
  • Target

    • Function

      Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.
    • Tissue specificity

      B-lymphocytes.
    • Sequence similarities

      Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
      Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Domain

      Contains 4 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
    • Post-translational
      modifications

      Phosphorylation of Tyr-762, Tyr-807 and Tyr-822 are involved in binding to SYK, GRB2 and SYK, respectively. Phosphorylation of Tyr-842 is involved in binding to SYK, PLCG2 and PIK3R1/PIK3R2.
      Phosphorylated on tyrosine residues by LYN.
    • Cellular localization

      Cell membrane.
    • Target information above from: UniProt accession P20273 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 933 Human
      • Omim: 107266 Human
      • SwissProt: P20273 Human
      • Unigene: 579691 Human
      • Alternative names

        • B cell receptor CD22 precursor antibody
        • B lymphocyte cell adhesion molecule antibody
        • B-cell receptor CD22 antibody
        • B-lymphocyte cell adhesion molecule antibody
        • BL CAM antibody
        • BL-CAM antibody
        • BLCAM antibody
        • CD 22 antibody
        • CD22 antibody
        • CD22 antigen antibody
        • CD22 molecule antibody
        • CD22 protein antibody
        • CD22_HUMAN antibody
        • Lectin 2 antibody
        • Leu14 antibody
        • Lyb8 antibody
        • MGC130020 antibody
        • sialic acid binding Ig like lectin 2 antibody
        • Sialic acid binding immunoglobulin like lectin 2 antibody
        • Sialic acid-binding Ig-like lectin 2 antibody
        • SIGLEC 2 antibody
        • Siglec-2 antibody
        • SIGLEC2 antibody
        • T cell surface antigen Leu 14 antibody
        • T-cell surface antigen Leu-14 antibody
        see all

      Images

      • Flow Cytometry - Anti-CD22 (phospho Y822) antibody [Y506] (ab32123)
        Flow Cytometry - Anti-CD22 (phospho Y822) antibody [Y506] (ab32123)
        Flow cytometric analysis of permeabilized K562 cells, untreated (green) or Pervanadate treated (red) using ab32123
      • Western blot - Anti-CD22 (phospho Y822) antibody [Y506] (ab32123)
        Western blot - Anti-CD22 (phospho Y822) antibody [Y506] (ab32123)
        All lanes : Anti-CD22 (phospho Y822) antibody [Y506] (ab32123) at 1/1000 dilution

        Lane 1 : Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate
        Lane 2 : Ramos treated with 1mM pervanadate for 30 min whole cell lysate
        Lane 3 : Ramos treated with 1mM pervanadate for 30 min whole cell lysate. Then the membrane was incubated with alkaline phosphatase.

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

        Predicted band size: 95 kDa
        Observed band size: 150 kDa
        why is the actual band size different from the predicted?



        Blocking and dilution buffer: 5% NFDM/TBST.

      • Dot Blot - Anti-CD22 (phospho Y822) antibody [Y506] (ab32123)
        Dot Blot - Anti-CD22 (phospho Y822) antibody [Y506] (ab32123)

        Dot blot analysis of CD22 (phospho Y822) phospho peptide (Lane 1) and CD22 non-phospho peptide (Lane 2) labelling CD22 (phospho Y822) with ab32123 at a dilution of 1/1000. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000. Blocking buffer: 5% NFDM/TBST. Dilution buffer: 5% NFDM /TBST.

      • Western blot - Anti-CD22 (phospho Y822) antibody [Y506] (ab32123)
        Western blot - Anti-CD22 (phospho Y822) antibody [Y506] (ab32123)
        All lanes : Anti-CD22 (phospho Y822) antibody [Y506] (ab32123) at 1/1000 dilution

        Lane 1 : Untreated Raji cell lysate
        Lane 2 : Raji cell lysate treated with Pervanadate

        Predicted band size: 95 kDa
        Observed band size: 150 kDa why is the actual band size different from the predicted?

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (2)

      Publishing research using ab32123? Please let us know so that we can cite the reference in this datasheet.

      ab32123 has been referenced in 2 publications.

      • Coughlin S  et al. An extracatalytic function of CD45 in B cells is mediated by CD22. Proc Natl Acad Sci U S A 112:E6515-24 (2015). PubMed: 26561584
      • Séïté JF  et al. IVIg modulates BCR signaling through CD22 and promotes apoptosis in mature human B lymphocytes. Blood 116:1698-704 (2010). Flow Cyt ; Human . PubMed: 20516366

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Flow Cytometry abreview for Anti-CD22 (phospho Y822) antibody [Y506]

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Flow Cytometry
      Sample
      Mouse Cell (Splenic B cells)
      Permeabilization
      Yes - BD Perm buffer III
      Gating Strategy
      Single cells, B220+ B cells
      Specification
      Splenic B cells
      Preparation
      Cell harvesting/tissue preparation method: Splenocyte isolation from fresh spleens followed by RBC lysis
      Sample buffer: Pervanadate stimulation
      Fixation
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Dec 02 2015

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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