Anti-CD22 (phospho Y822) antibody [Y506] (ab32123)
- Datasheet
- References (2)
- Protocols
Overview
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Product nameAnti-CD22 (phospho Y822) antibody [Y506]
See all CD22 primary antibodies -
DescriptionRabbit monoclonal [Y506] to CD22 (phospho Y822)
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Host speciesRabbit
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Specificityab32123 recognises CD22 phosphorylation on Tyrosine 822. This antibody is predicted to detect splice isoform 2 based on sequence homology.
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Tested applicationsSuitable for: WB, Flow Cyt, Dot blotmore details
Unsuitable for: IHC or IP -
Species reactivityReacts with: Human
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Immunogen
A synthetic phospho-peptide corresponding to residues surrounding Tyrosine 822 of human CD22
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Positive control
- WB: Ramos and Raji cell lysates treated with Pervanadate. Flow Cyt: K562 cells treated with Pervanadate.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA -
Concentration information loading... -
PurityIgG fraction
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ClonalityMonoclonal
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Clone numberY506
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IsotypeIgG
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Research areas
Associated products
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab32123 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 150 kDa (predicted molecular weight: 95 kDa). | |
| Flow Cyt | 1/10. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| Dot blot | 1/1000. |
Application notes
Is unsuitable for IHC or IP.
Target
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FunctionMediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.
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Tissue specificityB-lymphocytes.
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Sequence similaritiesBelongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
DomainContains 4 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
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Post-translational
modificationsPhosphorylation of Tyr-762, Tyr-807 and Tyr-822 are involved in binding to SYK, GRB2 and SYK, respectively. Phosphorylation of Tyr-842 is involved in binding to SYK, PLCG2 and PIK3R1/PIK3R2.
Phosphorylated on tyrosine residues by LYN. -
Cellular localizationCell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 933 Human
- Omim: 107266 Human
- SwissProt: P20273 Human
- Unigene: 579691 Human
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Alternative names
- B cell receptor CD22 precursor antibody
- B lymphocyte cell adhesion molecule antibody
- B-cell receptor CD22 antibody
see all
Images
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![Flow Cytometry - Anti-CD22 (phospho Y822) antibody [Y506] (ab32123)](https://www.abcam.com/ps/products/32/ab32123/Images/ab32123_2.jpg)
Flow Cytometry - Anti-CD22 (phospho Y822) antibody [Y506] (ab32123)Flow cytometric analysis of permeabilized K562 cells, untreated (green) or Pervanadate treated (red) using ab32123 -
![Western blot - Anti-CD22 (phospho Y822) antibody [Y506] (ab32123)](https://www.abcam.com/ps/products/32/ab32123/Images/ab32123-4-ab32123WB1.jpg)
Western blot - Anti-CD22 (phospho Y822) antibody [Y506] (ab32123)All lanes : Anti-CD22 (phospho Y822) antibody [Y506] (ab32123) at 1/1000 dilution
Lane 1 : Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 2 : Ramos treated with 1mM pervanadate for 30 min whole cell lysate
Lane 3 : Ramos treated with 1mM pervanadate for 30 min whole cell lysate. Then the membrane was incubated with alkaline phosphatase.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 95 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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![Dot Blot - Anti-CD22 (phospho Y822) antibody [Y506] (ab32123)](https://www.abcam.com/ps/products/32/ab32123/Images/ab32123-3-ab32123Dot1.jpg)
Dot Blot - Anti-CD22 (phospho Y822) antibody [Y506] (ab32123)Dot blot analysis of CD22 (phospho Y822) phospho peptide (Lane 1) and CD22 non-phospho peptide (Lane 2) labelling CD22 (phospho Y822) with ab32123 at a dilution of 1/1000. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000. Blocking buffer: 5% NFDM/TBST. Dilution buffer: 5% NFDM /TBST.
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![Western blot - Anti-CD22 (phospho Y822) antibody [Y506] (ab32123)](https://www.abcam.com/ps/products/32/ab32123/Images/ab32123_1.jpg)
Western blot - Anti-CD22 (phospho Y822) antibody [Y506] (ab32123)All lanes : Anti-CD22 (phospho Y822) antibody [Y506] (ab32123) at 1/1000 dilution
Lane 1 : Untreated Raji cell lysate
Lane 2 : Raji cell lysate treated with Pervanadate
Predicted band size: 95 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Datasheets and documents
References
This product has been referenced in:
- Coughlin S et al. An extracatalytic function of CD45 in B cells is mediated by CD22. Proc Natl Acad Sci U S A 112:E6515-24 (2015). Read more (PubMed: 26561584) »
- Séïté JF et al. IVIg modulates BCR signaling through CD22 and promotes apoptosis in mature human B lymphocytes. Blood 116:1698-704 (2010). Flow Cyt ; Human . Read more (PubMed: 20516366) »
