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    cd22-phospho-y842-antibody-y507-bsa-and-azide-free-ab247247.pdf

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Immunology Adaptive Immunity B Cells CD
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Recombinant
RabMAb

Recombinant Anti-CD22 (phospho Y842) antibody [Y507] - BSA and Azide free (ab247247)

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  • Datasheet
  • References
  • Protocols

Overview

  • Product name

    Anti-CD22 (phospho Y842) antibody [Y507] - BSA and Azide free
    See all CD22 primary antibodies
  • Description

    Rabbit monoclonal [Y507] to CD22 (phospho Y842) - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    The antibody detects CD22 phosphorylation on Tyrosine 842 and is predicted to detect splice isoforms 2 based on sequence homology.

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,IHC or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CD22 (phospho Y842). The exact sequence is proprietary.

  • General notes

    ab247247 is the carrier-free version of ab32355 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab247247 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    Y507
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • B Cells
    • CD
    • Cancer
    • Tumor immunology
    • Tumor-associated antigens
    • Cancer
    • Tumor biomarkers
    • Tumor antigens
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • B Lymphocytic Lineage

Associated products

  • Alternative Versions

    • Anti-CD22 (phospho Y842) antibody [Y507] (ab32355)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit (ab102918)
    • APC Conjugation Kit (ab201807)
  • Recombinant Protein

    • Recombinant Human CD22 protein (His tag) (ab217822)

Applications

Our Abpromise guarantee covers the use of ab247247 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 150 kDa (predicted molecular weight: 95 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,IHC or IP.
  • Target

    • Function

      Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.
    • Tissue specificity

      B-lymphocytes.
    • Sequence similarities

      Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
      Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Domain

      Contains 4 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
    • Post-translational
      modifications

      Phosphorylation of Tyr-762, Tyr-807 and Tyr-822 are involved in binding to SYK, GRB2 and SYK, respectively. Phosphorylation of Tyr-842 is involved in binding to SYK, PLCG2 and PIK3R1/PIK3R2.
      Phosphorylated on tyrosine residues by LYN.
    • Cellular localization

      Cell membrane.
    • Target information above from: UniProt accession P20273 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 933 Human
      • Omim: 107266 Human
      • SwissProt: P20273 Human
      • Unigene: 579691 Human
      • Alternative names

        • B cell receptor CD22 precursor antibody
        • B lymphocyte cell adhesion molecule antibody
        • B-cell receptor CD22 antibody
        • B-lymphocyte cell adhesion molecule antibody
        • BL CAM antibody
        • BL-CAM antibody
        • BLCAM antibody
        • CD 22 antibody
        • CD22 antibody
        • CD22 antigen antibody
        • CD22 molecule antibody
        • CD22 protein antibody
        • CD22_HUMAN antibody
        • Lectin 2 antibody
        • Leu14 antibody
        • Lyb8 antibody
        • MGC130020 antibody
        • sialic acid binding Ig like lectin 2 antibody
        • Sialic acid binding immunoglobulin like lectin 2 antibody
        • Sialic acid-binding Ig-like lectin 2 antibody
        • SIGLEC 2 antibody
        • Siglec-2 antibody
        • SIGLEC2 antibody
        • T cell surface antigen Leu 14 antibody
        • T-cell surface antigen Leu-14 antibody
        see all

      Datasheets and documents

      • Datasheet
    • References

      ab247247 has not yet been referenced specifically in any publications.

      Publishing research using ab247247? Please let us know so that we can cite the reference in this datasheet.

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