Anti-CD226 antibody [DX11] (ab33397)

Mouse monoclonal CD226 antibody [DX11]. Validated in IP, FuncS, Flow Cyt, BL and tested in Human. Cited in 3 publication(s). Immunogen corresponding to tissue, cells or virus.

Overview

  • Product name

    Anti-CD226 antibody [DX11]
    See all CD226 primary antibodies
  • Description

    Mouse monoclonal [DX11] to CD226
  • Host species

    Mouse
  • Tested applications

    Suitable for: Flow Cyt, Functional Studies, IP, Blockingmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue/ cell preparation (Human) - cytotoxic T lymphocyte clone.

  • General notes


    This antibody is reported to inhibit T- and NK cell mediated cytotoxicity against tumour cell targets and to block TNF alpha and IFN gamma secretion by alloantigen-specific T-cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: None
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
  • Primary antibody notes

    This antibody is reported to inhibit T- and NK cell mediated cytotoxicity against tumour cell targets and to block TNF alpha and IFN gamma secretion by alloantigen-specific T-cells.
  • Clonality

    Monoclonal
  • Clone number

    DX11
  • Myeloma

    Sp2/0
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab33397 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Functional Studies Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Blocking Use at an assay dependent concentration. PubMed: 23980210

Target

  • Function

    Receptor involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cell.
  • Tissue specificity

    Expressed by peripheral blood T-lymphocytes.
  • Sequence similarities

    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • adhesion glycoprotein antibody
    • CD226 antibody
    • CD226 antigen antibody
    • CD226 molecule antibody
    • CD226_HUMAN antibody
    • DNAM 1 antibody
    • DNAM-1 antibody
    • DNAM1 antibody
    • DNAX accessory molecule 1 antibody
    • platelet and T cell activation antigen 1 antibody
    • PTA1 antibody
    • T lineage specific activation antigen 1 antigen antibody
    • TLiSA1 antibody
    see all

Images

  • Human peripheral blood lymphocytes stained with ab33397, using 10µl of the working dilution for 106 cells in 100µl.

References

This product has been referenced in:

  • Xu F  et al. Blockade of CD112R and TIGIT signaling sensitizes human natural killer cell functions. Cancer Immunol Immunother 66:1367-1375 (2017). Read more (PubMed: 28623459) »
  • Wong JL  et al. IL-18-primed helper NK cells collaborate with dendritic cells to promote recruitment of effector CD8+ T cells to the tumor microenvironment. Cancer Res 73:4653-62 (2013). Read more (PubMed: 23761327) »
See all 5 Publications for this product

Customer reviews and Q&As

Answer

A description of the production and characterization of the CD226 antibody, clone DX11, and the statement regarding inhibition and blocking, are in the following reference.


Shibuya, A. et al. (1996) DNAM-1, a novel adhesion molecule involved in the cytolytic function of T lymphocytes.

Immunity. 4 (6): 573-81.  PMID: 8673704


Here is a link to the full text. http://www.cell.com/immunity/fulltext/S1074-7613(00)70060-4

Read More

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