Anti-CD226 antibody [DX11] (ab33397)
Key features and details
- Mouse monoclonal [DX11] to CD226
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Anti-CD226 antibody [DX11]
See all CD226 primary antibodies -
Description
Mouse monoclonal [DX11] to CD226 -
Host species
Mouse -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human CD226. Human cytotoxic T lymphocyte clone.
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General notes
This antibody is reported to inhibit T- and NK cell mediated cytotoxicity against tumour cell targets and to block TNF alpha and IFN gamma secretion by alloantigen-specific T-cells.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant -
Primary antibody notes
This antibody is reported to inhibit T- and NK cell mediated cytotoxicity against tumour cell targets and to block TNF alpha and IFN gamma secretion by alloantigen-specific T-cells. -
Clonality
Monoclonal -
Clone number
DX11 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab33397 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use 10µl for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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Flow Cyt
Use 10µl for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Receptor involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cell. -
Tissue specificity
Expressed by peripheral blood T-lymphocytes. -
Sequence similarities
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 10666 Human
- Omim: 605397 Human
- SwissProt: Q15762 Human
- Unigene: 660130 Human
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Alternative names
- adhesion glycoprotein antibody
- CD226 antibody
- CD226 antigen antibody
see all
Images
Datasheets and documents
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Datasheet download
References (7)
ab33397 has been referenced in 7 publications.
- Xu F et al. Blockade of CD112R and TIGIT signaling sensitizes human natural killer cell functions. Cancer Immunol Immunother 66:1367-1375 (2017). PubMed: 28623459
- Zhu Y et al. Identification of CD112R as a novel checkpoint for human T cells. J Exp Med 213:167-76 (2016). PubMed: 26755705
- Sugai S et al. Hepatocellular carcinoma cell sensitivity to V?9Vd2 T lymphocyte-mediated killing is increased by zoledronate. Int J Oncol 48:1794-804 (2016). PubMed: 26936487
- Lozano E et al. The CD226/CD155 interaction regulates the proinflammatory (Th1/Th17)/anti-inflammatory (Th2) balance in humans. J Immunol 191:3673-80 (2013). Blocking ; Human . PubMed: 23980210
- Wong JL et al. IL-18-primed helper NK cells collaborate with dendritic cells to promote recruitment of effector CD8+ T cells to the tumor microenvironment. Cancer Res 73:4653-62 (2013). PubMed: 23761327
- Lozano E et al. The TIGIT/CD226 axis regulates human T cell function. J Immunol 188:3869-75 (2012). PubMed: 22427644
- Ravanat C et al. Use of tandem Biacore-mass spectrometry to identify platelet membrane targets of novel monoclonal antibodies. Anal Biochem 386:237-43 (2009). Flow Cyt ; Human . PubMed: 19135971