Overview

  • Product name

    Anti-CD226 antibody [EPR21172]
    See all CD226 primary antibodies
  • Description

    Rabbit monoclonal [EPR21172] to CD226
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CD226 aa 100-200. The exact sequence is proprietary.
    Database link: Q15762

  • Positive control

    • WB: HEL whole cell lysate; Human fetal spleen and thymus lysates. IHC-P: Human bone marrow and spleen tissues.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab212076 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 60-80 kDa (predicted molecular weight: 39 kDa).
IHC-P 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Receptor involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cell.
  • Tissue specificity

    Expressed by peripheral blood T-lymphocytes.
  • Sequence similarities

    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • adhesion glycoprotein antibody
    • CD226 antibody
    • CD226 antigen antibody
    • CD226 molecule antibody
    • CD226_HUMAN antibody
    • DNAM 1 antibody
    • DNAM-1 antibody
    • DNAM1 antibody
    • DNAX accessory molecule 1 antibody
    • platelet and T cell activation antigen 1 antibody
    • PTA1 antibody
    • T lineage specific activation antigen 1 antigen antibody
    • TLiSA1 antibody
    see all

Images

  • All lanes : Anti-CD226 antibody [EPR21172] (ab212076) at 1/1000 dilution

    Lane 1 : HEL (human bone marrow erythroleukemia cell line) whole cell lysate
    Lane 2 : Human fetal spleen lysate
    Lane 3 : Human thymus lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 39 kDa
    Observed band size: 60-80 kDa
    why is the actual band size different from the predicted?



    Exposure time : Lane 1: 37 seconds; Lane 2: 15 seconds; Lane 3: 3 minutes.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The different molecular masses observed in different tissues or cell lines may be due to different level of CD226 glycosylation (PMID: 8673704).

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling CD226 with ab212076 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining of platelets and endothelium in human spleen is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Perform heat-induced antigen retrieval using ab208572 (Universal HIER antigen retrieval reagent).

  • Immunohistochemical analysis of paraffin-embedded human bone marrow tissue labeling CD226 with ab212076 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in megakaryocytes of human bone marrow (PMID: 15693793) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Perform heat-induced antigen retrieval using ab208572 (Universal HIER antigen retrieval reagent).

References

ab212076 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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