Recombinant
RabMAb

Recombinant Anti-CD226 antibody [EPR21172] - BSA and Azide free (ab230865)

Overview

  • Product name

    Anti-CD226 antibody [EPR21172] - BSA and Azide free
    See all CD226 primary antibodies
  • Description

    Rabbit monoclonal [EPR21172] to CD226 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CD226 aa 100-200. The exact sequence is proprietary.
    Database link: Q15762

  • Positive control

    • IHC-P: Human bone marrow tissue.
  • General notes

    Ab230865 is the carrier-free version of ab212076. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab230865 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab230865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Detects a band of approximately 60-80 kDa (predicted molecular weight: 39 kDa).

Target

  • Function

    Receptor involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cell.
  • Tissue specificity

    Expressed by peripheral blood T-lymphocytes.
  • Sequence similarities

    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • adhesion glycoprotein antibody
    • CD226 antibody
    • CD226 antigen antibody
    • CD226 molecule antibody
    • CD226_HUMAN antibody
    • DNAM 1 antibody
    • DNAM-1 antibody
    • DNAM1 antibody
    • DNAX accessory molecule 1 antibody
    • platelet and T cell activation antigen 1 antibody
    • PTA1 antibody
    • T lineage specific activation antigen 1 antigen antibody
    • TLiSA1 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling CD226 with ab212076 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining of platelets and endothelium in human spleen is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Perform heat-induced antigen retrieval using ab208572 (Universal HIER antigen retrieval reagent).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab212076).

  • Immunohistochemical analysis of paraffin-embedded human bone marrow tissue labeling CD226 with ab212076 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in megakaryocytes of human bone marrow (PMID: 15693793). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Perform heat-induced antigen retrieval using ab208572 (Universal HIER antigen retrieval reagent).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab212076).

References

ab230865 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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