Product nameAnti-CD226 antibody [EPR21243]
See all CD226 primary antibodies
DescriptionRabbit monoclonal [EPR21243] to CD226
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Human
Tissue, cells or virus.
Database link: Q15762
- Flow Cyt: Jurkat cells
This product was made using synthetic libraries and phage display technology.
This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
- Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
Our Abpromise guarantee covers the use of ab242030 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use a concentration of 1 µg/ml.|
FunctionReceptor involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cell.
Tissue specificityExpressed by peripheral blood T-lymphocytes.
Sequence similaritiesContains 2 Ig-like C2-type (immunoglobulin-like) domains.
- Information by UniProt
- adhesion glycoprotein antibody
- CD226 antibody
- CD226 antigen antibody
Overlay histograms showing left Jurkat and right MCF-7 cells stained with ab242030 (red line). The cells were incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab242030, 1x106 cells in 100ul at 1ug/ml) for 30 min on ice. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) preabsorbed (4ug/ml) for 30 min on ice.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 20 mW Blue laser (488nm) and 530/30 bandpass filter.
Negative control: MCF-7 cells.
ab242030 has not yet been referenced specifically in any publications.