Recombinant Anti-CD226 antibody [EPR21245] (ab245186)
![Flow Cytometry - Anti-CD226 antibody [EPR21245] (ab245186)](https://www.abcam.com/ps/products/245/ab245186/Images/ab245186-336991-anti-cd226-antibody-epr21245-flow-cytometry.jpg "Flow Cytometry - Anti-CD226 antibody [EPR21245] (ab245186)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21245] to CD226
- Suitable for: Flow Cyt
- Reacts with: Mouse
Overview
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Product name
Anti-CD226 antibody [EPR21245]
See all CD226 primary antibodies -
Description
Rabbit monoclonal [EPR21245] to CD226 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is considered to be commercially sensitive.
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Positive control
- Flow Cyt: C57BL/6 mouse splenocytes
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General notes
This product was made using synthetic libraries and phage display technology.
This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR21245 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab245186 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use a concentration of 1 µg/ml.
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| Notes |
|---|
|
Flow Cyt
Use a concentration of 1 µg/ml. |
Target
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Function
Receptor involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cell. -
Tissue specificity
Expressed by peripheral blood T-lymphocytes. -
Sequence similarities
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 225825 Mouse
- SwissProt: Q8K4F0 Mouse
- Unigene: 139293 Mouse
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Alternative names
- adhesion glycoprotein antibody
- CD226 antibody
- CD226 antigen antibody
see all
Images
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Flow Cytometry - Anti-CD226 antibody [EPR21245] (ab245186)
C57BL/6 mouse splenocytes stained with ab245186 (right) or chimeric Rabbit IgG (monoclonal) (left). C57BL/6 Mouse splenocytes were incubated for 30min on ice in 1x PBS containing 10ug/ml anti CD16/32 antibody and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab245186) or chimeric Rabbit IgG Isotype (1x106 in 100ul at 1ug/ml) for 30 min on ice.
The secondary antibody Goat anti-rabbit IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150081) was used at 1/2000 dilution for 30 min on ice.The cells were simultaneously stained with CD3 Alexa® 700 conjugated antibody.
Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.
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![Anti-CD226 antibody [EPR21245] (ab245186)](https://www.abcam.com/ps/products/245/ab245186/Images/ab245186-2-benefits-of-recombinant-antibodies.png)
Anti-CD226 antibody [EPR21245] (ab245186)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab245186 has not yet been referenced specifically in any publications.
