APC Anti-CD23 antibody [2G8] (ab25457)
Key features and details
- APC Rat monoclonal [2G8] to CD23
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: APC. Ex: 645nm, Em: 660nm
- Isotype: IgG2a
Overview
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Product name
APC Anti-CD23 antibody [2G8]
See all CD23 primary antibodies -
Description
APC Rat monoclonal [2G8] to CD23 -
Host species
Rat -
Conjugation
APC. Ex: 645nm, Em: 660nm -
Specificity
ab25457 recognises CD23 low affinity IgE Fc receptor. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to CD23. FcεR isolated from B cell lymphoma cell line M12.4.5
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.3
Preservative: 0.09% Sodium azide
Constituents: PBS, 16% Sucrose
Stabilising agent. -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
2G8 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25457 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Notes |
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Note: ab25457 can be used for identification and enumeration of CD23+ cells by flow cytometry and for differentiation of B cell subpopulations by cell sorting.
IP: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
This receptor has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen). -
Sequence similarities
Contains 1 C-type lectin domain. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Cell membrane. Secreted. Also exists as a soluble excreted form. - Information by UniProt
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Database links
- Entrez Gene: 14128 Mouse
- SwissProt: P20693 Mouse
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Alternative names
- Blast 2 antibody
- BLAST-2 antibody
- Blast2 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab25457 has been referenced in 1 publication.
- Hamel K et al. Suppression of Proteoglycan-Induced Arthritis by Anti-CD20 B Cell Depletion Therapy Is Mediated by Reduction in Autoantibodies and CD4+ T Cell Reactivity. J Immunol 180:4994-5003 (2008). Flow Cyt ; Mouse . PubMed: 18354225