Recombinant
RabMAb

Recombinant Anti-CD23 antibody [EPR3617] (ab92495)

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Overview

  • Product name

    Anti-CD23 antibody [EPR3617]
    See all CD23 primary antibodies

  • Description

    Rabbit monoclonal [EPR3617] to CD23

  • Host species

    Rabbit

  • Specificity

    Recognizes both membrane bound and soluble forms of CD23.

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human CD23 aa 300-400 (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: Human lymph and tonsil lysates; IHC-P: Human tonsil tissue; FC: Human peripheral blood mononuclear cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab92495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 36 kDa.
IHC-P 1/400. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/00 - 1/250.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    This receptor has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen).

  • Sequence similarities

    Contains 1 C-type lectin domain.

  • Post-translational
    modifications

    N- and O-glycosylated.

  • Cellular localization

    Cell membrane. Secreted. Also exists as a soluble excreted form.
  • Information by UniProt

  • Database links

    • Alternative names

      • Blast 2 antibody
      • BLAST-2 antibody
      • Blast2 antibody
      • C type lectin domain family 4 member J antibody
      • C-type lectin domain family 4 antibody
      • C-type lectin domain family 4 member J antibody
      • C-type lectin domain family 4, member J antibody
      • CD 23 antibody
      • CD 23A antibody
      • CD23 antibody
      • CD23 antigen antibody
      • CD23A antibody
      • CLEC 4J antibody
      • CLEC4J antibody
      • Fc epsilon receptor II antibody
      • Fc epsilon RII antibody
      • Fc fragment of IgE antibody
      • Fc fragment of IgE low affinity II receptor for antibody
      • Fc fragment of IgE receptor II antibody
      • Fc fragment of IgE, low affinity II, receptor for (CD23) antibody
      • Fc of IgE antibody
      • Fc of IgE, low affinity II, receptor for (CD23) antibody
      • Fc receptor IgE low affinity II alpha polypeptide antibody
      • Fc receptor, IgE, low affinity II, alpha polypeptide, isoform CRA_a antibody
      • Fc-epsilon-RII antibody
      • FCE 2 antibody
      • FCE2 antibody
      • FCER 2 antibody
      • Fcer2 antibody
      • FCER2_HUMAN antibody
      • FCER2A antibody
      • FceRII antibody
      • IgE binding factor antibody
      • IgE receptor lymphocyte antibody
      • IgE-binding factor antibody
      • IGEBF antibody
      • Immunoglobulin E binding factor antibody
      • Immunoglobulin E receptor antibody
      • Immunoglobulin E receptor, low affinity II antibody
      • Immunoglobulin E-binding factor antibody
      • Immunoglobulin epsilon chain antibody
      • LEUKOCYTE ANTIGEN CD23 antibody
      • Low Affinity IgE Receptor antibody
      • Low affinity immunoglobulin epsilon Fc receptor antibody
      • Low affinity immunoglobulin epsilon Fc receptor membrane bound form antibody
      • Low affinity immunoglobulin epsilon Fc receptor soluble form antibody
      • Ly-42 antibody
      • Ly42 antibody
      • Lymphocyte antigen CD23 antibody
      • Lymphocyte IgE receptor antibody
      • MGC93219 antibody
      see all

    Images

    • Western blot - Anti-CD23 antibody [EPR3617] (ab92495)
      Western blot - Anti-CD23 antibody [EPR3617] (ab92495)
      Anti-CD23 antibody [EPR3617] (ab92495) at 1/1000 dilution (Purified) + Human lymph lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 36 kDa
      Observed band size: 37,45 kDa
      why is the actual band size different from the predicted?



      The bands detected by ab92495 are consistent with what have been described in PMID: 20876574 and PMID: 17389606.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (ab92495)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (ab92495)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling CD23 with Purified ab92495 at 1/400 dilution (0.5 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    • Flow Cytometry - Anti-CD23 antibody [EPR3617] (ab92495)
      Flow Cytometry - Anti-CD23 antibody [EPR3617] (ab92495)

      Flow Cytometry analysis of Human peripheral blood mononuclear (PBMC) cells labeling CD23 with purified ab92495 at 1:20 dilution (10 µg/ml) (Red). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Left).

      Cells were stained with rabbit IgG (Left) or ab92495 (Right). Then stained with anti-CD19 conjugated to Alexa Fluor® 647. Gated on viable cells.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (ab92495)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (ab92495)

      ab92495 (unpurified), at a 1/100 dilution, staining CD23 in formalin fixed, paraffin embedded Human tonsil tissue by Immunohistochemistry. Detection: DAB staining.

      Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (ab92495)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (ab92495)

      ab92495 (unpurified) showing positive staining in Normal tonsil tissue.

      Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (ab92495)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (ab92495)

      ab92495 (unpurified) showing negative staining in Normal heart tissue.

      Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (ab92495)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (ab92495)

      ab92495 (unpurified) showing negative staining in Normal breast tissue.

      Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (ab92495)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (ab92495)

      ab92495 (unpurified) showing negative staining in Normal brain tissue.

      Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.

    • Other - Anti-CD23 antibody [EPR3617] (ab92495)
      Other - Anti-CD23 antibody [EPR3617] (ab92495)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab92495 has not yet been referenced specifically in any publications.

    Publishing research using ab92495? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Question

    Dear Sir/Madam Please kindly recommend the abcam slide control for IHC-P application for this antibodies. 1. Anti-ErbB 2 antibody (ab2428) 2. Anti-Progesterone Receptor antibody [SP2] (ab16661) 3. Anti-Estrogen Receptor antibody [SP1] (ab16660) 4. Anti-Myeloperoxidase antibody (ab45977) 5. Anti-TdT antibody (ab85148) 6. Anti-TMEM16A antibody (ab53212) 7. Anti-NSE antibody [EPR3377] (ab79757) 8. Anti-CD23 antibody [EPR3617(IHC)] (ab92495) 9. Anti-CD10 antibody [56C6] (ab951) 10. Anti-Melanoma antibody [HMB45 + DT101 + BC199] (ab732) 11. Anti-CD79a antibody [HM47/A9] (ab3121) 12. Anti-CD45RO antibody [UCHL-1] (ab86080) 13. Anti-CD45 antibody (ab10559) 14. Anti-CD30 antibody [CON6D/ C2] - BSA and Azide free (ab76518) 15. Anti-CD15 antibody [(Workshop IV) (BRA-4F1) our designation 238)] (ab115993) Best regards

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    Answer

    Thank you for your enquiry. I am pleased to provide the following suggested positive controls for these antibodies. I have mainly chosen tissue that has been tested succesfully and shown in an image on the datasheets: 1.Anti-ErbB 2 antibody (ab2428) Human breast cancer 2.Anti-Progesterone Receptor antibody [SP2] (ab16661) Human breast carcinoma 3.Anti-Estrogen Receptor antibody [SP1] (ab16660) Human breast carcinoma 4.Anti-Myeloperoxidase antibody (ab45977) Human spleen tissue 5.Anti-TdT antibody (ab85148) Human testis 6.Anti-TMEM16A antibody (ab53212) Human liver 7.Anti-NSE antibody [EPR3377] (ab79757) Human brain tissue 8.Anti-CD23 antibody [EPR3617(IHC)] (ab92495) Human tonsil 9.Anti-CD10 antibody [56C6] (ab951) Lymph node tissue or human breast microepithelium 10.Anti-Melanoma antibody [HMB45 + DT101 + BC199] (ab732) Murine melanoma metastasis tissue 11.Anti-CD79a antibody [HM47/A9] (ab3121) Human Lymphoid tissue, tonsil 12.Anti-CD45RO antibody [UCHL-1] (ab86080) Human Tonsil, Thymus 13.Anti-CD45 antibody (ab10559) Human Tonsil tissue 14.Anti-CD30 antibody [CON6D/ C2] - BSA and Azide free (ab76518) Human Hodgekins lymphoma tissue 15.Anti-CD15 antibody [(Workshop IV) (BRA-4F1) our designation 238)] (ab115993) Human Hodgekins lymphoma tissue Human spleen I am providing a list ofslides that we sell which I hope will be helpful to you. I can recommend to review the online datasheet for more details. Human brain: ab4305Brain (human): hippocampus normal tissue slides https://www.abcam.com/index.html?datasheet=4305 (or use the following: https://www.abcam.com/index.html?datasheet=4305). Human lymph node: ab4350Lymph node (human) normal tissue slides IHC-P, ISH https://www.abcam.com/index.html?datasheet=4350 (or use the following: https://www.abcam.com/index.html?datasheet=4350). Human testis: ab4373Testis (human) normal tissue slides IHC-P, ISH https://www.abcam.com/index.html?datasheet=4373 (or use the following: https://www.abcam.com/index.html?datasheet=4373). Human liver: ab4348Liver (human) normal tissue slides IHC-P, ISH https://www.abcam.com/index.html?datasheet=4348 (or use the following: https://www.abcam.com/index.html?datasheet=4348). Human spleen: ab4372Spleen (human) normal tissue slides IHC-P, ISH https://www.abcam.com/index.html?datasheet=4372 (or use the following: https://www.abcam.com/index.html?datasheet=4372). I am sorry we do not have slides forthe following: Human tonsil Hodgekins lymphoma tissue Mouse melenoma tissue I hope this will be helpful to you. If you have any further questions,please do not hesitate to contact us.

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