Recombinant Anti-CD23 antibody [EPR3617] (ab92495)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3617] to CD23
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-CD23 antibody [EPR3617]
See all CD23 primary antibodies -
Description
Rabbit monoclonal [EPR3617] to CD23 -
Host species
Rabbit -
Specificity
Recognizes both membrane bound and soluble forms of CD23. -
Tested applications
Suitable for: WB, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human CD23 aa 300-400 (C terminal). The exact sequence is proprietary.
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Positive control
- WB: Human lymph and tonsil lysates; IHC-P: Human tonsil tissue; Flow Cyt: Human peripheral blood mononuclear cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Dissociation constant (KD)
KD = 5.50 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3617 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab92495 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Predicted molecular weight: 36 kDa. | |
| IHC-P | 1/400. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/00 - 1/250. |
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| Flow Cyt | 1/20. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Function
This receptor has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen). -
Sequence similarities
Contains 1 C-type lectin domain. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Cell membrane. Secreted. Also exists as a soluble excreted form. - Information by UniProt
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Database links
- Entrez Gene: 2208 Human
- Omim: 151445 Human
- SwissProt: P06734 Human
- Unigene: 465778 Human
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Alternative names
- Blast 2 antibody
- BLAST-2 antibody
- Blast2 antibody
see all
Images
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![Western blot - Anti-CD23 antibody [EPR3617] (ab92495)](https://www.abcam.com/ps/products/92/ab92495/Images/ab92495-352515-anti-cd23-antibody-epr3617-westernblot-lymph-human.jpg)
Western blot - Anti-CD23 antibody [EPR3617] (ab92495)Anti-CD23 antibody [EPR3617] (ab92495) at 1/1000 dilution (Purified) + Human lymph lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 36 kDa
Observed band size: 37,45 kDa why is the actual band size different from the predicted?The bands detected by ab92495 are consistent with what have been described in PMID: 20876574 and PMID: 17389606.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (ab92495)](https://www.abcam.com/ps/products/92/ab92495/Images/ab92495-10-anti-cd23-antibody-epr3617-immunohistochemistry-tonsil-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (ab92495)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling CD23 with Purified ab92495 at 1/400 dilution (0.5 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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![Flow Cytometry - Anti-CD23 antibody [EPR3617] (ab92495)](https://www.abcam.com/ps/products/92/ab92495/Images/ab92495-11-anti-cd23-antibody-epr3617-flowcytometry-pbmc-human.jpg)
Flow Cytometry - Anti-CD23 antibody [EPR3617] (ab92495)Flow Cytometry analysis of Human peripheral blood mononuclear (PBMC) cells labeling CD23 with purified ab92495 at 1:20 dilution (10 µg/ml) (Red). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Left).
Cells were stained with rabbit IgG (Left) or ab92495 (Right). Then stained with anti-CD19 conjugated to Alexa Fluor® 647. Gated on viable cells.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (ab92495)](https://www.abcam.com/ps/products/92/ab92495/Images/ab92495-107546-anti-cd23-antibody-epr3617-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (ab92495)ab92495 (unpurified), at a 1/100 dilution, staining CD23 in formalin fixed, paraffin embedded Human tonsil tissue by Immunohistochemistry. Detection: DAB staining.
Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (ab92495)](https://www.abcam.com/ps/products/92/ab92495/Images/ab92495-107545-anti-cd23-antibody-epr3617-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (ab92495)ab92495 (unpurified) showing positive staining in Normal tonsil tissue.
Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (ab92495)](https://www.abcam.com/ps/products/92/ab92495/Images/ab92495-107544-anti-cd23-antibody-epr3617-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (ab92495)ab92495 (unpurified) showing negative staining in Normal heart tissue.
Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (ab92495)](https://www.abcam.com/ps/products/92/ab92495/Images/ab92495-107543-anti-cd23-antibody-epr3617-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (ab92495)ab92495 (unpurified) showing negative staining in Normal breast tissue.
Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (ab92495)](https://www.abcam.com/ps/products/92/ab92495/Images/ab92495-107542-anti-cd23-antibody-epr3617-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (ab92495)ab92495 (unpurified) showing negative staining in Normal brain tissue.
Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-CD23 antibody [EPR3617] (ab92495)](https://www.abcam.com/ps/products/92/ab92495/Images/ab92495-107541-anti-cd23-antibody-epr3617.jpg)
OI-RD Scanning - Anti-CD23 antibody [EPR3617] (ab92495) -
![Anti-CD23 antibody [EPR3617] (ab92495)](https://www.abcam.com/ps/products/92/ab92495/Images/ab92495-12-benefits-of-recombinant-antibodies.png)
Anti-CD23 antibody [EPR3617] (ab92495)
Protocols
Datasheets and documents
References (1)
ab92495 has been referenced in 1 publication.
- Yang X et al. Misdiagnosis of primary pleural DLBCL as tuberculosis: A case report and literature review. Mol Clin Oncol 8:729-732 (2018). PubMed: 29732155
