Overview

  • Product name

    Anti-CD23 antibody [SP163]
    See all CD23 primary antibodies
  • Description

    Rabbit monoclonal [SP163] to CD23
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human CD23 aa 1-250. The exact sequence is proprietary.
    Database link: P06734

  • Positive control

    • Flow Cyt: Raji cells. WB: Raji cell lysate. IHC-P: Human tonsil tissue. ICC/IF: Raji cells
  • General notes

Properties

Applications

Our Abpromise guarantee covers the use of ab135386 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/25.
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB 1/25. Predicted molecular weight: 36 kDa.
IHC-P 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    This receptor has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen).
  • Sequence similarities

    Contains 1 C-type lectin domain.
  • Post-translational
    modifications

    N- and O-glycosylated.
  • Cellular localization

    Cell membrane. Secreted. Also exists as a soluble excreted form.
  • Information by UniProt
  • Database links

  • Alternative names

    • Blast 2 antibody
    • BLAST-2 antibody
    • Blast2 antibody
    • C type lectin domain family 4 member J antibody
    • C-type lectin domain family 4 member J antibody
    • C-type lectin domain family 4, member J antibody
    • CD 23 antibody
    • CD 23A antibody
    • CD23 antibody
    • CD23 antigen antibody
    • CD23A antibody
    • CLEC 4J antibody
    • CLEC4J antibody
    • Fc epsilon receptor II antibody
    • Fc epsilon RII antibody
    • Fc fragment of IgE low affinity II receptor for antibody
    • Fc fragment of IgE receptor II antibody
    • Fc fragment of IgE, low affinity II, receptor for (CD23) antibody
    • Fc of IgE, low affinity II, receptor for (CD23) antibody
    • Fc receptor IgE low affinity II alpha polypeptide antibody
    • Fc receptor, IgE, low affinity II, alpha polypeptide, isoform CRA_a antibody
    • Fc-epsilon-RII antibody
    • FCE 2 antibody
    • FCE2 antibody
    • FCER 2 antibody
    • Fcer2 antibody
    • FCER2_HUMAN antibody
    • FCER2A antibody
    • FceRII antibody
    • IgE binding factor antibody
    • IgE receptor lymphocyte antibody
    • IgE-binding factor antibody
    • IGEBF antibody
    • Immunoglobulin E binding factor antibody
    • Immunoglobulin E receptor, low affinity II antibody
    • Immunoglobulin E-binding factor antibody
    • Immunoglobulin epsilon chain antibody
    • LEUKOCYTE ANTIGEN CD23 antibody
    • Low Affinity IgE Receptor antibody
    • Low affinity immunoglobulin epsilon Fc receptor antibody
    • Low affinity immunoglobulin epsilon Fc receptor membrane bound form antibody
    • Low affinity immunoglobulin epsilon Fc receptor soluble form antibody
    • Ly-42 antibody
    • Ly42 antibody
    • Lymphocyte antigen CD23 antibody
    • Lymphocyte IgE receptor antibody
    • MGC93219 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence analysis of Raji (human Burkitt's lymphoma B lymphocyte) cells labeling CD23 with purified ab135386 (7.48 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow cytometric analysis of rabbit anti-CD23 (SP163) antibody ab135386 (1/100) in Raji (Human Burkitt's lymphoma cell line) cells (green) compared to negative control of rabbit IgG (blue).

  • Anti-CD23 antibody [SP163] (ab135386) at 1/25 dilution + Raji (Human Burkitt's lymphoma cell line) cell lysate

    Predicted band size: 36 kDa

  • Immunohistochemical analysis of paraffin embedded human tonsil tissue labeling CD23 with ab135386 antibody at a dilution of 1/50.

References

ab135386 has not yet been referenced specifically in any publications.

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