RabMAb

Anti-CD23 antibody [SP23] (ab16702)

Submit a review Q&A (1)References (1)

Overview

  • Product name
    Anti-CD23 antibody [SP23]
    See all CD23 primary antibodies
  • Description
    Rabbit monoclonal [SP23] to CD23
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to CD23.

  • Positive control
    • Human tonsil. Raji cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab16702 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
IHC-P 1/100. Retrieve antigens with 70% Formic acid for 10-30 minutes at room temperature before commencing with IHC staining protocol. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    This receptor has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen).
  • Sequence similarities
    Contains 1 C-type lectin domain.
  • Post-translational
    modifications
    N- and O-glycosylated.
  • Cellular localization
    Cell membrane. Secreted. Also exists as a soluble excreted form.
  • Information by UniProt
  • Database links
    • Alternative names
      • Blast 2 antibody
      • BLAST-2 antibody
      • Blast2 antibody
      • C type lectin domain family 4 member J antibody
      • C-type lectin domain family 4 member J antibody
      • C-type lectin domain family 4, member J antibody
      • CD 23 antibody
      • CD 23A antibody
      • CD23 antibody
      • CD23 antigen antibody
      • CD23A antibody
      • CLEC 4J antibody
      • CLEC4J antibody
      • Fc epsilon receptor II antibody
      • Fc epsilon RII antibody
      • Fc fragment of IgE low affinity II receptor for antibody
      • Fc fragment of IgE receptor II antibody
      • Fc fragment of IgE, low affinity II, receptor for (CD23) antibody
      • Fc of IgE, low affinity II, receptor for (CD23) antibody
      • Fc receptor IgE low affinity II alpha polypeptide antibody
      • Fc receptor, IgE, low affinity II, alpha polypeptide, isoform CRA_a antibody
      • Fc-epsilon-RII antibody
      • FCE 2 antibody
      • FCE2 antibody
      • FCER 2 antibody
      • Fcer2 antibody
      • FCER2_HUMAN antibody
      • FCER2A antibody
      • FceRII antibody
      • IgE binding factor antibody
      • IgE receptor lymphocyte antibody
      • IgE-binding factor antibody
      • IGEBF antibody
      • Immunoglobulin E binding factor antibody
      • Immunoglobulin E receptor, low affinity II antibody
      • Immunoglobulin E-binding factor antibody
      • Immunoglobulin epsilon chain antibody
      • LEUKOCYTE ANTIGEN CD23 antibody
      • Low Affinity IgE Receptor antibody
      • Low affinity immunoglobulin epsilon Fc receptor antibody
      • Low affinity immunoglobulin epsilon Fc receptor membrane bound form antibody
      • Low affinity immunoglobulin epsilon Fc receptor soluble form antibody
      • Ly-42 antibody
      • Ly42 antibody
      • Lymphocyte antigen CD23 antibody
      • Lymphocyte IgE receptor antibody
      • MGC93219 antibody
      see all

    Images

    • Flow Cytometry - Anti-CD23 antibody [SP23] (ab16702)
      Flow Cytometry - Anti-CD23 antibody [SP23] (ab16702)

      Flow cytometric analysis of rabbit anti-CD23 (SP23) antibody ab16702 (1/100) in Jurkats (green) compared to negative control of rabbit IgG (blue).

    • Western blot - Anti-CD23 antibody [SP23] (ab16702)
      Western blot - Anti-CD23 antibody [SP23] (ab16702)
      Anti-CD23 antibody [SP23] (ab16702) at 1/25 dilution + lysate prepared from Raji cells

      Observed band size: 45 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [SP23] (ab16702)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [SP23] (ab16702)
      ab16702 at 1/100 dilution staining human CD23 in tonsil by Immunohistochemistry (FFPE-sections).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [SP23] (ab16702)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [SP23] (ab16702)

      Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling CD23 with ab137883 at 1/100.

    References

    This product has been referenced in:
    • Koletsa T  et al. Synchronous Presence of Nasopharyngeal Carcinoma and Marginal Zone (MALT-Type) B-Cell Lymphoma in the Pharynx. Patholog Res Int 2011:340763 (2011). Read more (PubMed: 21660262) »
    See 1 Publication for this product

    Publishing research using ab16702? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Question

    Further to my mail relating to rabbit monoclonal CD23 (ab16702), I thought you might like to know that this antibody works brilliantly when retrieved in the microwave in high pH buffer and using the primary at your recommended 1:100. As a lot of labs don't use formic acid retrieval, I thought this might be useful info for you in case anyone else asks.

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    Answer

    Thank you very much for this feedback this is most useful. I would encourage (if you have not already done so) to take the time to submit an "Abreview" in order to get rewarded for this feedback. The submission of feedback via our online system should take approximately 15 minutes. In exchange for feedback via this system you can earn up to 300 points. The link below will provide you with some further details on the submission of Abreviews and the “prizes” that you can get for your points. http://it.abcam.com/index.html?pageconfig=resource&rid=10332&pid=10000 Should you have any questions in relation to this then please do get back in touch with me.

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