Anti-CD23 antibody [SP23] (ab16702)
![Flow Cytometry - Anti-CD23 antibody [SP23] (ab16702)](https://www.abcam.com/ps/products/16/ab16702/Images/ab16702-215233-anti-cd23-antibody-sp23-flow-cytometry.jpg "Flow Cytometry - Anti-CD23 antibody [SP23] (ab16702)")
Key features and details
- Rabbit monoclonal [SP23] to CD23
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CD23 antibody [SP23]
See all CD23 primary antibodies -
Description
Rabbit monoclonal [SP23] to CD23 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human CD23 aa 1-250. The exact sequence is proprietary.
Database link: P06734 -
Positive control
- Human tonsil. Raji cell lysate
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.50
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
SP23 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab16702 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration.
|
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| IHC-P |
1/100.
Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. |
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| Flow Cyt |
Use at an assay dependent concentration.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
WB
Use at an assay dependent concentration. |
|
IHC-P
1/100. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. |
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Flow Cyt
Use at an assay dependent concentration. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
This receptor has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen). -
Sequence similarities
Contains 1 C-type lectin domain. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Cell membrane. Secreted. Also exists as a soluble excreted form. - Information by UniProt
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Database links
- Entrez Gene: 2208 Human
- Omim: 151445 Human
- SwissProt: P06734 Human
- Unigene: 465778 Human
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Alternative names
- Blast 2 antibody
- BLAST-2 antibody
- Blast2 antibody
see all
Images
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Flow Cytometry - Anti-CD23 antibody [SP23] (ab16702)
Flow cytometric analysis of rabbit anti-CD23 (SP23) antibody ab16702 (1/100) in Jurkats (green) compared to negative control of rabbit IgG (blue).
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![Western blot - Anti-CD23 antibody [SP23] (ab16702)](https://www.abcam.com/ps/products/16/ab16702/Images/ab16702-25557-anti-cd23-antibody-sp23-western-blot.jpg)
Western blot - Anti-CD23 antibody [SP23] (ab16702)Anti-CD23 antibody [SP23] (ab16702) at 1/25 dilution + lysate prepared from Raji cells
Observed band size: 45 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [SP23] (ab16702)](https://www.abcam.com/ps/products/16/ab16702/Images/ab16702-25558-anti-cd23-antibody-sp23-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [SP23] (ab16702)ab16702 at 1/100 dilution staining human CD23 in tonsil by Immunohistochemistry (FFPE-sections). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [SP23] (ab16702)](https://www.abcam.com/ps/products/16/ab16702/Images/ab16702-210741-anti-cd23-antibody-sp23-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [SP23] (ab16702)Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling CD23 with ab137883 at 1/100.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab16702 has been referenced in 2 publications.
- Fukano C et al. Establishment of enzyme-linked immunosorbent facilitated antigen binding as a biomarker assay for Japanese cedar pollen allergy immunotherapy. J Pharmacol Sci 140:223-227 (2019). PubMed: 31353210
- Koletsa T et al. Synchronous Presence of Nasopharyngeal Carcinoma and Marginal Zone (MALT-Type) B-Cell Lymphoma in the Pharynx. Patholog Res Int 2011:340763 (2011). PubMed: 21660262