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Anti-CD23 antibody [SP23] - BSA and Azide free (ab242380)

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Flow Cytometry - Anti-CD23 antibody [SP23] - BSA and Azide free (ab242380)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [SP23] - BSA and Azide free (ab242380)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [SP23] - BSA and Azide free (ab242380)

Key features and details

  • Rabbit monoclonal [SP23] to CD23 - BSA and Azide free
  • Suitable for: Flow Cyt, IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

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Conjugation
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PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)

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Overview

  • Product name

    Anti-CD23 antibody [SP23] - BSA and Azide free
    See all CD23 primary antibodies
  • Description

    Rabbit monoclonal [SP23] to CD23 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to CD23.

  • Positive control

    • IHC-P: Human tonsil tissue. Flow Cyt: Jurkat cells.
  • General notes

    FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    Ab242380 is the carrier-free version of ab16702. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab242380 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.20
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    SP23
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • B Cells
    • CD
    • Immunology
    • Immunoglobulins
    • Receptors
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • B Lymphocytic Lineage

Associated products

  • Alternative Versions

    • Anti-CD23 antibody [SP23] (ab16702)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Positive Controls

    • Raji whole cell lysate (ab30124)

Applications

Our Abpromise guarantee covers the use of ab242380 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration.

Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

WB Use at an assay dependent concentration. Predicted molecular weight: 36 kDa.

Target

  • Function

    This receptor has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen).
  • Sequence similarities

    Contains 1 C-type lectin domain.
  • Post-translational
    modifications

    N- and O-glycosylated.
  • Cellular localization

    Cell membrane. Secreted. Also exists as a soluble excreted form.
  • Target information above from: UniProt accession P06734 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2208 Human
    • Omim: 151445 Human
    • SwissProt: P06734 Human
    • Unigene: 465778 Human
    • Alternative names

      • Blast 2 antibody
      • BLAST-2 antibody
      • Blast2 antibody
      • C type lectin domain family 4 member J antibody
      • C-type lectin domain family 4 antibody
      • C-type lectin domain family 4 member J antibody
      • C-type lectin domain family 4, member J antibody
      • CD 23 antibody
      • CD 23A antibody
      • CD23 antibody
      • CD23 antigen antibody
      • CD23A antibody
      • CLEC 4J antibody
      • CLEC4J antibody
      • Fc epsilon receptor II antibody
      • Fc epsilon RII antibody
      • Fc fragment of IgE antibody
      • Fc fragment of IgE low affinity II receptor for antibody
      • Fc fragment of IgE receptor II antibody
      • Fc fragment of IgE, low affinity II, receptor for (CD23) antibody
      • Fc of IgE antibody
      • Fc of IgE, low affinity II, receptor for (CD23) antibody
      • Fc receptor IgE low affinity II alpha polypeptide antibody
      • Fc receptor, IgE, low affinity II, alpha polypeptide, isoform CRA_a antibody
      • Fc-epsilon-RII antibody
      • FCE 2 antibody
      • FCE2 antibody
      • FCER 2 antibody
      • Fcer2 antibody
      • FCER2_HUMAN antibody
      • FCER2A antibody
      • FceRII antibody
      • IgE binding factor antibody
      • IgE receptor lymphocyte antibody
      • IgE-binding factor antibody
      • IGEBF antibody
      • Immunoglobulin E binding factor antibody
      • Immunoglobulin E receptor antibody
      • Immunoglobulin E receptor, low affinity II antibody
      • Immunoglobulin E-binding factor antibody
      • Immunoglobulin epsilon chain antibody
      • LEUKOCYTE ANTIGEN CD23 antibody
      • Low Affinity IgE Receptor antibody
      • Low affinity immunoglobulin epsilon Fc receptor antibody
      • Low affinity immunoglobulin epsilon Fc receptor membrane bound form antibody
      • Low affinity immunoglobulin epsilon Fc receptor soluble form antibody
      • Ly-42 antibody
      • Ly42 antibody
      • Lymphocyte antigen CD23 antibody
      • Lymphocyte IgE receptor antibody
      • MGC93219 antibody
      see all

    Images

    • Flow Cytometry - Anti-CD23 antibody [SP23] - BSA and Azide free (ab242380)
      Flow Cytometry - Anti-CD23 antibody [SP23] - BSA and Azide free (ab242380)

      Flow cytometric analysis of rabbit anti-CD23 (SP23) antibody ab16702 (1/100) in Jurkats (green) compared to negative control of rabbit IgG (blue).

      This data was developed using the same antibody clone in a different buffer formulation containing Tris buffered saline, BSA, and sodium azide (ab16702).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [SP23] - BSA and Azide free (ab242380)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [SP23] - BSA and Azide free (ab242380)

      Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling CD23 with ab137883 at 1/100.

      This data was developed using the same antibody clone in a different buffer formulation containing Tris buffered saline, BSA, and sodium azide (ab16702).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [SP23] - BSA and Azide free (ab242380)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [SP23] - BSA and Azide free (ab242380)

      ab16702 at 1/100 dilution staining human CD23 in tonsil by Immunohistochemistry (FFPE-sections).

      This data was developed using the same antibody clone in a different buffer formulation containing Tris buffered saline, BSA, and sodium azide (ab16702).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab242380? Please let us know so that we can cite the reference in this datasheet.

    ab242380 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    There are currently no Customer reviews or Questions for ab242380.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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