Anti-CD23 antibody [SP23] - BSA and Azide free (ab242380)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-CD23 antibody [SP23] - BSA and Azide free
See all CD23 primary antibodies -
Description
Rabbit monoclonal [SP23] to CD23 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to CD23.
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Positive control
- IHC-P: Human tonsil tissue. Flow Cyt: Jurkat cells.
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General notes
Ab242380 is the carrier-free version of ab16702. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab242380 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
SP23 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab242380 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| IHC-P | Use at an assay dependent concentration. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. |
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| WB | Use at an assay dependent concentration. Predicted molecular weight: 36 kDa. |
Target
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Function
This receptor has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen). -
Sequence similarities
Contains 1 C-type lectin domain. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Cell membrane. Secreted. Also exists as a soluble excreted form. - Information by UniProt
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Database links
- Entrez Gene: 2208 Human
- Omim: 151445 Human
- SwissProt: P06734 Human
- Unigene: 465778 Human
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Alternative names
- Blast 2 antibody
- BLAST-2 antibody
- Blast2 antibody
see all
Images
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![Flow Cytometry - Anti-CD23 antibody [SP23] - BSA and Azide free (ab242380)](https://www.abcam.com/ps/products/242/ab242380/Images/ab242380-331870-anti-cd23-antibody-sp23-flow-cytometry.jpg)
Flow Cytometry - Anti-CD23 antibody [SP23] - BSA and Azide free (ab242380)Flow cytometric analysis of rabbit anti-CD23 (SP23) antibody ab16702 (1/100) in Jurkats (green) compared to negative control of rabbit IgG (blue).
This data was developed using the same antibody clone in a different buffer formulation containing Tris buffered saline, BSA, and sodium azide (ab16702).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [SP23] - BSA and Azide free (ab242380)](https://www.abcam.com/ps/products/242/ab242380/Images/ab242380-331869-anti-cd23-antibody-sp23-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [SP23] - BSA and Azide free (ab242380) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [SP23] - BSA and Azide free (ab242380)](https://www.abcam.com/ps/products/242/ab242380/Images/ab242380-331868-anti-cd23-antibody-sp23-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [SP23] - BSA and Azide free (ab242380)
Datasheets and documents
References
ab242380 has not yet been referenced specifically in any publications.
