Anti-CD239/BCAM antibody (ab244280)
Key features and details
- Rabbit polyclonal to CD239/BCAM
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CD239/BCAM antibody
See all CD239/BCAM primary antibodies -
Description
Rabbit polyclonal to CD239/BCAM -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human CD239/BCAM aa 300-450.
Database link: P50895 -
Positive control
- IHC-P: Human Fallopian tube, kidney and pancreas tissue. ICC/IF: A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab244280 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
|
IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Laminin alpha-5 receptor. May mediate intracellular signaling. -
Tissue specificity
Wide tissue distribution (highest in the pancreas and very low in brain). Closely associated with the basal layer of cells in epithelia and the endothelium of blood vessel walls. -
Sequence similarities
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 2 Ig-like V-type (immunoglobulin-like) domains. -
Developmental stage
Is under developmental control in liver and may also be regulated during differentiation in other tissues. Up-regulated following malignant transformation in some cell types. -
Post-translational
modificationsEpinephrine-stimulated phosphorylation of Ser-621 by PKA enhances adhesion to laminin. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 4059 Human
- Omim: 612773 Human
- SwissProt: P50895 Human
- Unigene: 625725 Human
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Alternative names
- Antigen identified by monoclonal antibody F8 antibody
- AU antibody
- Auberger B antigen antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human Fallopian tube labeling CD239/BCAM with ab244280 at a 1/200 dilution.
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PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for CD239/BCAM (green) using ab244280 at 4 µg/ml in ICC/IF.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human kidney tissue labeling CD239/BCAM with ab244280 at a 1/200 dilution.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human pancreas tissue labeling CD239/BCAM with ab244280 at a 1/200 dilution.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human liver tissue labeling CD239/BCAM with ab244280 at a 1/200 dilution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab244280 has been referenced in 1 publication.
- Shannon MJ et al. Cell trajectory modeling identifies a primitive trophoblast state defined by BCAM enrichment. Development 149:N/A (2022). PubMed: 35020896