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    cd239bcam-antibody-epr23603-74-bsa-and-azide-free-detector-ab305358.pdf

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Cardiovascular Blood Blood Cell Antigens
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-CD239/BCAM antibody [EPR23603-74] - BSA and Azide free (Detector) (ab305358)

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Sandwich ELISA - Anti-CD239/BCAM antibody [EPR23603-74] - BSA and Azide free (Detector) (ab305358)

    Key features and details

    • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
    • Rabbit monoclonal [EPR23603-74] to CD239/BCAM - BSA and Azide free (Detector)
    • Suitable for: Sandwich ELISA
    • Knockout validated
    • Reacts with: Human

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    Overview

    • Product name

      Anti-CD239/BCAM antibody [EPR23603-74] - BSA and Azide free (Detector)
      See all CD239/BCAM primary antibodies
    • Description

      Rabbit monoclonal [EPR23603-74] to CD239/BCAM - BSA and Azide free (Detector)
    • Host species

      Rabbit
    • Tested applications

      Suitable for: Sandwich ELISAmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

    • General notes

      ab305358 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human CD239/BCAM.  

      The recommended pair for sandwich ELISA is:

      Capture: ab305357 , Human CD239/BCAM Capture Antibody (unconjugated)

      Detector: ab305358 , Human CD239/BCAM Detector Antibody (unconjugated)

      The reference range value is 1.56-1000pg/ml.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      pH: 7.20
      Constituent: 100% PBS
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Monoclonal
    • Clone number

      EPR23603-74
    • Isotype

      IgG
    • Research areas

      • Cardiovascular
      • Blood
      • Blood Cell Antigens
      • Cardiovascular
      • Blood
      • Other
      • Immunology
      • Immunoglobulins
      • Receptors
      • Cardiovascular
      • Atherosclerosis
      • Thrombosis
      • Other

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
      • Goat Anti-Rabbit IgG H&L (Biotin) (ab207995)
      • Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab6722)
    • Conjugation kits

      • Alkaline phosphatase Conjugation Kit - Lightning-Link® (ab102850)
      • HRP Conjugation Kit - Lightning-Link® (ab102890)
      • Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (ab201795)
    • sELISA pair antibody

      • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
      • Streptavidin Conjugation Kit - Lightning-Link® (ab102921)
      • Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (ab201795)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab305358 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Sandwich ELISA
    Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795).
    Notes
    Sandwich ELISA
    Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795).

    Target

    • Function

      Laminin alpha-5 receptor. May mediate intracellular signaling.
    • Tissue specificity

      Wide tissue distribution (highest in the pancreas and very low in brain). Closely associated with the basal layer of cells in epithelia and the endothelium of blood vessel walls.
    • Sequence similarities

      Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 2 Ig-like V-type (immunoglobulin-like) domains.
    • Developmental stage

      Is under developmental control in liver and may also be regulated during differentiation in other tissues. Up-regulated following malignant transformation in some cell types.
    • Post-translational
      modifications

      Epinephrine-stimulated phosphorylation of Ser-621 by PKA enhances adhesion to laminin.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P50895 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 4059 Human
      • Omim: 612773 Human
      • SwissProt: P50895 Human
      • Unigene: 625725 Human
      • Alternative names

        • Antigen identified by monoclonal antibody F8 antibody
        • AU antibody
        • Auberger B antigen antibody
        • B CAM cell surface glycoprotein antibody
        • B cell adhesion molecule antibody
        • B-CAM cell surface glycoprotein antibody
        • Basal cell adhesion molecule (Lu and Au blood groups) antibody
        • Basal cell adhesion molecule (Lutheran blood group) antibody
        • Basal cell adhesion molecule antibody
        • Basal cell adhesion molecule Lu and Au blood groups antibody
        • Basal cell adhesion molecule Lutheran blood group antibody
        • Bcam antibody
        • BCAM_HUMAN antibody
        • CD239 antibody
        • CD239 antigen antibody
        • F8/G253 antigen antibody
        • Glycoprotein 95kDa antibody
        • LU antibody
        • Lutheran antibody
        • Lutheran antigen antibody
        • Lutheran blood group (Auberger b antigen included) antibody
        • Lutheran blood group Auberger b antigen included antibody
        • Lutheran blood group glycoprotein antibody
        • MSK19 antibody
        see all

      Images

      • Sandwich ELISA - Anti-CD239/BCAM antibody [EPR23603-74] - BSA and Azide free (Detector) (ab305358)
        Sandwich ELISA - Anti-CD239/BCAM antibody [EPR23603-74] - BSA and Azide free (Detector) (ab305358)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet download

        Download

      References (0)

      Publishing research using ab305358? Please let us know so that we can cite the reference in this datasheet.

      ab305358 has not yet been referenced specifically in any publications.

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