Recombinant Anti-CD239/BCAM antibody [EPR4164] (ab111181)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-CD239/BCAM antibody [EPR4164]
See all CD239/BCAM primary antibodies -
Description
Rabbit monoclonal [EPR4164] to CD239/BCAM -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICCmore details
Unsuitable for: Flow Cyt,IP or WB -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human CD239/BCAM aa 50-150. The exact sequence is proprietary.
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Positive control
- Human Breast Carcinoma and Tonsil Tissues.
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General notes
This product was previously labelled as CD239
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4164 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab111181 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. | |
| ICC | 1/100 - 1/250. |
Target
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Function
Laminin alpha-5 receptor. May mediate intracellular signaling. -
Tissue specificity
Wide tissue distribution (highest in the pancreas and very low in brain). Closely associated with the basal layer of cells in epithelia and the endothelium of blood vessel walls. -
Sequence similarities
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 2 Ig-like V-type (immunoglobulin-like) domains. -
Developmental stage
Is under developmental control in liver and may also be regulated during differentiation in other tissues. Up-regulated following malignant transformation in some cell types. -
Post-translational
modificationsEpinephrine-stimulated phosphorylation of Ser-621 by PKA enhances adhesion to laminin. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 4059 Human
- Omim: 612773 Human
- SwissProt: P50895 Human
- Unigene: 625725 Human
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Alternative names
- Antigen identified by monoclonal antibody F8 antibody
- AU antibody
- Auberger B antigen antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD239/BCAM antibody [EPR4164] (ab111181)](https://www.abcam.com/ps/products/111/ab111181/Images/CD239-Primary-antibodies-ab111181-4.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD239/BCAM antibody [EPR4164] (ab111181)ab111181 at 1/100 dilution staining CD239/BCAM in Paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD239/BCAM antibody [EPR4164] (ab111181)](https://www.abcam.com/ps/products/111/ab111181/Images/CD239-Primary-antibodies-ab111181-5.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD239/BCAM antibody [EPR4164] (ab111181)ab111181 at 1/100 dilution staining CD239/BCAM in Paraffin-embedded Human tonsil tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD239/BCAM antibody [EPR4164] (ab111181)](https://www.abcam.com/ps/products/111/ab111181/Images/ab111181-124483-anti-cd239-antibody-epr4164-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD239/BCAM antibody [EPR4164] (ab111181)ab111181 showing positive staining in Thyroid gland carcinoma tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD239/BCAM antibody [EPR4164] (ab111181)](https://www.abcam.com/ps/products/111/ab111181/Images/CD239-Primary-antibodies-ab111181-7.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD239/BCAM antibody [EPR4164] (ab111181)ab111181 showing positive staining in Normal stomach tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD239/BCAM antibody [EPR4164] (ab111181)](https://www.abcam.com/ps/products/111/ab111181/Images/CD239-Primary-antibodies-ab111181-8.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD239/BCAM antibody [EPR4164] (ab111181)ab111181 showing positive staining in Normal ovary tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD239/BCAM antibody [EPR4164] (ab111181)](https://www.abcam.com/ps/products/111/ab111181/Images/CD239-Primary-antibodies-ab111181-9.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD239/BCAM antibody [EPR4164] (ab111181)ab111181 showing positive staining in Normal liver tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD239/BCAM antibody [EPR4164] (ab111181)](https://www.abcam.com/ps/products/111/ab111181/Images/CD239-Primary-antibodies-ab111181-10.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD239/BCAM antibody [EPR4164] (ab111181)ab111181 showing positive staining in Normal kidney tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD239/BCAM antibody [EPR4164] (ab111181)](https://www.abcam.com/ps/products/111/ab111181/Images/CD239-Primary-antibodies-ab111181-11.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD239/BCAM antibody [EPR4164] (ab111181)ab111181 showing positive staining in Hepatocellular carcinoma tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD239/BCAM antibody [EPR4164] (ab111181)](https://www.abcam.com/ps/products/111/ab111181/Images/CD239-Primary-antibodies-ab111181-12.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD239/BCAM antibody [EPR4164] (ab111181)ab111181 showing negative staining in Normal heart tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Datasheets and documents
References
This product has been referenced in:
- Tang R et al. Differentiation of human induced pluripotent stem cells into nucleus pulposus-like cells. Stem Cell Res Ther 9:61 (2018). Read more (PubMed: 29523190) »
