Recombinant Anti-CD239/BCAM antibody [EPR4165] (ab134110)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4165] to CD239/BCAM
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CD239/BCAM antibody [EPR4165]
See all CD239/BCAM primary antibodies -
Description
Rabbit monoclonal [EPR4165] to CD239/BCAM -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- 293T cell lysate, HeLa whole cell lysate (ab150035), A431 cell lysate, Human tonsil tissue ICC/IF: A431 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4165 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134110 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (3) |
1/1000 - 1/10000. Detects a band of approximately 85 kDa (predicted molecular weight: 67 kDa).
|
IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Perform antigen retrieval before commencing with IHC staining protocol |
|
ICC/IF | (3) |
1/100.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 85 kDa (predicted molecular weight: 67 kDa). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform antigen retrieval before commencing with IHC staining protocol |
ICC/IF
1/100. |
Target
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Function
Laminin alpha-5 receptor. May mediate intracellular signaling. -
Tissue specificity
Wide tissue distribution (highest in the pancreas and very low in brain). Closely associated with the basal layer of cells in epithelia and the endothelium of blood vessel walls. -
Sequence similarities
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 2 Ig-like V-type (immunoglobulin-like) domains. -
Developmental stage
Is under developmental control in liver and may also be regulated during differentiation in other tissues. Up-regulated following malignant transformation in some cell types. -
Post-translational
modificationsEpinephrine-stimulated phosphorylation of Ser-621 by PKA enhances adhesion to laminin. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 4059 Human
- Entrez Gene: 57278 Mouse
- Entrez Gene: 78958 Rat
- Omim: 612773 Human
- SwissProt: P50895 Human
- SwissProt: Q9R069 Mouse
- SwissProt: Q9ESS6 Rat
- Unigene: 625725 Human
see all -
Alternative names
- Antigen identified by monoclonal antibody F8 antibody
- AU antibody
- Auberger B antigen antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence analysis of A431 (human epidermoid carcinoma epithelial cell) labeling CD239/BCAM with ab134110 at 1/100 dilution followed by ab150077 at 1/1000 dilution. Cells were fixed with 4% Paraformaldehyde and permeabilied with 0.1% TritonX-100. DAPI was used as nuclear counter stain. Cells were counterstained wth ab195889 at 1/200 dilution.Confocal image showing mainly membranous staining in A431 cell line.
Negative control: JurKat (PMID:30078440)
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). -
Immunohistochemical analysis of paraffin embedded Human tonsil tissue labelling CD239/BCAM with ab134110 at 1/250.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-CD239/BCAM antibody [EPR4165] (ab134110) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 67 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?Secondary antibody - goat anti-rabbit HRP (ab6721)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab134110 has been referenced in 3 publications.
- Sun M et al. Application of a novel targeting nanoparticle contrast agent combined with magnetic resonance imaging in the diagnosis of intraductal papillary mucinous neoplasm. Exp Ther Med 16:1216-1224 (2018). PubMed: 30116372
- Sprague L et al. Dendritic cells: In vitro culture in two- and three-dimensional collagen systems and expression of collagen receptors in tumors and atherosclerotic microenvironments. Exp Cell Res 323:7-27 (2014). Flow Cyt ; Mouse . PubMed: 24569142
- Timpe LC et al. Systemic alteration of cell-surface and secreted glycoprotein expression in malignant breast cancer cell lines. Glycobiology 23:1240-9 (2013). PubMed: 23918816