Recombinant
RabMAb

Recombinant Anti-CD239/BCAM antibody [EPR4165] - BSA and Azide free (ab226152)

Overview

  • Product name

    Anti-CD239/BCAM antibody [EPR4165] - BSA and Azide free
    See all CD239/BCAM primary antibodies
  • Description

    Rabbit monoclonal [EPR4165] to CD239/BCAM - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, Flow Cytmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CD239/BCAM aa 600-700 (C terminal). The exact sequence is proprietary.

  • Positive control

    • 293T cell lysate, HeLa cell lysate, A431 cell lysate, Human tonsil tissue
  • General notes

    ab226152 is the carrier-free version of ab134110 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab226152 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as CD239

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab226152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 85 kDa (predicted molecular weight: 67 kDa).
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Perform antigen retrieval before commencing with IHC staining protocol

Flow Cyt Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function

      Laminin alpha-5 receptor. May mediate intracellular signaling.
    • Tissue specificity

      Wide tissue distribution (highest in the pancreas and very low in brain). Closely associated with the basal layer of cells in epithelia and the endothelium of blood vessel walls.
    • Sequence similarities

      Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 2 Ig-like V-type (immunoglobulin-like) domains.
    • Developmental stage

      Is under developmental control in liver and may also be regulated during differentiation in other tissues. Up-regulated following malignant transformation in some cell types.
    • Post-translational
      modifications

      Epinephrine-stimulated phosphorylation of Ser-621 by PKA enhances adhesion to laminin.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Antigen identified by monoclonal antibody F8 antibody
      • AU antibody
      • Auberger B antigen antibody
      • B CAM cell surface glycoprotein antibody
      • B cell adhesion molecule antibody
      • B-CAM cell surface glycoprotein antibody
      • Basal cell adhesion molecule (Lu and Au blood groups) antibody
      • Basal cell adhesion molecule (Lutheran blood group) antibody
      • Basal cell adhesion molecule antibody
      • Basal cell adhesion molecule Lu and Au blood groups antibody
      • Basal cell adhesion molecule Lutheran blood group antibody
      • Bcam antibody
      • BCAM_HUMAN antibody
      • CD239 antibody
      • CD239 antigen antibody
      • F8/G253 antigen antibody
      • Glycoprotein 95kDa antibody
      • LU antibody
      • Lutheran antibody
      • Lutheran antigen antibody
      • Lutheran blood group (Auberger b antigen included) antibody
      • Lutheran blood group Auberger b antigen included antibody
      • Lutheran blood group glycoprotein antibody
      • MSK19 antibody
      see all

    References

    ab226152 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab226152.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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