Anti-CD24 antibody [ALB 9] (ab31622)

Knockout Tested Mouse monoclonal CD24 antibody [ALB 9]. Validated in IHC, ICC, Flow Cyt and tested in Human. Cited in 12 publication(s). Independently reviewed in 2 review(s).

Overview

  • Product name

    Anti-CD24 antibody [ALB 9]
    See all CD24 primary antibodies
  • Description

    Mouse monoclonal [ALB 9] to CD24
  • Host species

    Mouse
  • Specificity

    The clone number has been updated from (8.B.76) to (ALB 9) both clone numbers name the same antibody clone.
  • Tested applications

    Suitable for: ICC, Flow Cyt, IHC-Fr, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Human CD24.

  • Positive control

    • Flow Cyt: Live HAP1 wildtype cells. IHC-P: Human colon tissue.
  • General notes

    CD24 expression can also be observed in the cytoplasm.

    ab31622 binds to the non-glycosylated GPI anchor of the protein core of the cluster w4/CD24. It recognizes B cells from pre-B stage to mature-B stage, the majority of normal medullar granulous cells, peripheral blood polymorphonuclear cells, non-T, non-B acute lymphoblastic leukemias, some atypic or myeloid leukemias or B chronic lymphocytic leukemias and two-thirds of Epstein-Barr virus transformed cell lines.

     

Properties

Applications

Our Abpromise guarantee covers the use of ab31622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/25 - 1/50. Cell smear.
Flow Cyt Use a concentration of 10 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IHC-Fr 1/25 - 1/50.
IHC-P Use at an assay dependent concentration.

Target

  • Function

    Modulates B-cell activation responses. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells.
  • Tissue specificity

    B-cells. Expressed in a number of B-cell lines including P32/SH and Namalwa. Expressed in erythroleukemia cell and small cell lung carcinoma cell lines. Also expressed on the surface of T-cells.
  • Involvement in disease

    Genetic variations in CD24 are associated with susceptibility to multiple sclerosis (MS) [MIM:126200]. A multifactorial, inflammatory, demyelinating disease of the central nervous system. Sclerotic lesions are characterized by perivascular infiltration of monocytes and lymphocytes and appear as indurated areas in pathologic specimens (sclerosis in plaques). The pathological mechanism is regarded as an autoimmune attack of the myelin sheat, mediated by both cellular and humoral immunity. Clinical manifestations include visual loss, extra-ocular movement disorders, paresthesias, loss of sensation, weakness, dysarthria, spasticity, ataxia and bladder dysfunction. Genetic and environmental factors influence susceptibility to the disease. Note=Polymorphisms in CD24 may act as a genetic modifier for susceptibility and progression of MS in some populations, perhaps by affecting the efficiency of CD24 expression on the cell surface.
  • Sequence similarities

    Belongs to the CD24 family.
  • Post-translational
    modifications

    Extensively O-glycosylated.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD 24 antibody
    • CD24 antibody
    • CD24 antigen (small cell lung carcinoma cluster 4 antigen) antibody
    • CD24 antigen antibody
    • CD24 molecule antibody
    • CD24_HUMAN antibody
    • CD24A antibody
    • FLJ22950 antibody
    • FLJ43543 antibody
    • GPI linked surface mucin antibody
    • Heat stable antigen antibody
    • HSA antibody
    • MGC75043 antibody
    • Nectadrin antibody
    • Signal transducer CD24 antibody
    • Small cell lung carcinoma cluster 4 antigen antibody
    see all

Images

  • Overlay histogram showing HAP1 wildtype (green line) and HAP1-CD24 knockout cells (red line) stained with ab31622. Live HAP1 wildtype and HAP1-CD24 knockout cells were incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab31622, 10µg/0.5x106 cells) for 30 min at 22°C. A mouse IgG1 isotype control antibody  (ab170190) was used at the same concentration and conditions as the primary antibody (HAP1 wildtype - black line, HAP1-ITGB1 knockout - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity). Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

  • ab31622 staining CD24 in human colon tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde, then a heat mediated antigen retrieval step was performed using citrate buffer pH 6 (20 minutes). Samples were then incubated with the primary antibody at a 1/100 dilution for 16 minutes at 25°C. An undiluted HRP-conjugated goat anti-mouse/rabbit monoclonal was used as secondary antibody.

    See Abreview

References

This product has been referenced in:

  • Zhang W  et al. Androgen receptor/let-7a signaling regulates breast tumor-initiating cells. Oncotarget 9:3690-3703 (2018). Read more (PubMed: 29423076) »
  • Song Y  et al. Sirtuin 3-dependent mitochondrial redox homeostasis protects against AGEs-induced intervertebral disc degeneration. Redox Biol 19:339-353 (2018). Read more (PubMed: 30216853) »
See all 13 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Ph=6
Sample
Human Tissue sections (Normal breast tissue, MCF-7 and MDA-MB-231 cell li)
Specification
Normal breast tissue, MCF-7 and MDA-MB-231 cell li
Permeabilization
No
Fixative
Formaldehyde

Dr. Hazem Ghebeh

Verified customer

Submitted Sep 16 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (colon, normal and cancer)
Specification
colon, normal and cancer
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH6, 20 min
Permeabilization
No

Abcam user community

Verified customer

Submitted May 11 2010

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