Recombinant
RabMAb

Recombinant Anti-CD24 antibody [EPR19925] - BSA and Azide free (ab228455)

Overview

  • Product name

    Anti-CD24 antibody [EPR19925] - BSA and Azide free
    See all CD24 primary antibodies
  • Description

    Rabbit monoclonal [EPR19925] to CD24 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CD24 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P25063

  • Positive control

    • ICC/IF: MCF7 cells.
  • General notes

    Ab228455 is the carrier-free version of ab202073. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab228455 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab228455 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

Target

  • Function

    Modulates B-cell activation responses. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells.
  • Tissue specificity

    B-cells. Expressed in a number of B-cell lines including P32/SH and Namalwa. Expressed in erythroleukemia cell and small cell lung carcinoma cell lines. Also expressed on the surface of T-cells.
  • Involvement in disease

    Genetic variations in CD24 are associated with susceptibility to multiple sclerosis (MS) [MIM:126200]. A multifactorial, inflammatory, demyelinating disease of the central nervous system. Sclerotic lesions are characterized by perivascular infiltration of monocytes and lymphocytes and appear as indurated areas in pathologic specimens (sclerosis in plaques). The pathological mechanism is regarded as an autoimmune attack of the myelin sheat, mediated by both cellular and humoral immunity. Clinical manifestations include visual loss, extra-ocular movement disorders, paresthesias, loss of sensation, weakness, dysarthria, spasticity, ataxia and bladder dysfunction. Genetic and environmental factors influence susceptibility to the disease. Note=Polymorphisms in CD24 may act as a genetic modifier for susceptibility and progression of MS in some populations, perhaps by affecting the efficiency of CD24 expression on the cell surface.
  • Sequence similarities

    Belongs to the CD24 family.
  • Post-translational
    modifications

    Extensively O-glycosylated.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD 24 antibody
    • CD24 antibody
    • CD24 antigen (small cell lung carcinoma cluster 4 antigen) antibody
    • CD24 antigen antibody
    • CD24 molecule antibody
    • CD24_HUMAN antibody
    • CD24A antibody
    • FLJ22950 antibody
    • FLJ43543 antibody
    • GPI linked surface mucin antibody
    • Heat stable antigen antibody
    • HSA antibody
    • MGC75043 antibody
    • Nectadrin antibody
    • Signal transducer CD24 antibody
    • Small cell lung carcinoma cluster 4 antigen antibody
    see all

Images

  • Flow cytometric analysis of MDA-MB-231 (human breast adenocarcinoma cell line, Left) and MCF7 breast adenocarcinoma cell line, Right) cell lines labeling CD24 with ab202073 at 1/500 (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    Gated on total viable cells. Negative control: MDA-MB-231 (PMID: 18433506 and 22934288).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab202073).

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (human breast adenocarcinoma cell line) and MDA-MB-231 (human breast adenocarcinoma cell line) cells labeling CD24 with ab202073 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on MCF7 cell line.

    Negative control: MDA-MB-231 (PMID: 18433506).

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab202073).

References

ab228455 has not yet been referenced specifically in any publications.

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