Anti-CD24 antibody [SN3] (ab134375)
![Flow Cytometry - Anti-CD24 antibody [SN3] (ab134375)](https://www.abcam.com/ps/products/134/ab134375/Images/ab134375-414784-anti-cd24-antibody-sn3-flow-cytometry-peripheral-blood-human.jpg "Flow Cytometry - Anti-CD24 antibody [SN3] (ab134375)")
Key features and details
- Mouse monoclonal [SN3] to CD24
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Anti-CD24 antibody [SN3]
See all CD24 primary antibodies -
Description
Mouse monoclonal [SN3] to CD24 -
Host species
Mouse -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified) corresponding to Human CD24. Glycoproteins purified from Human NALM-1 cell line.
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Positive control
- Flow Cyt: Human peripheral blood cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purity: >95% (by SDS-PAGE). -
Clonality
Monoclonal -
Clone number
SN3 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
- APC/Cy7® Anti-CD24 antibody [SN3], prediluted (ab197137)
- APC Anti-CD24 antibody [SN3] (ab239286)
- PerCP/Cy5.5® Anti-CD24 antibody [SN3] (ab239339)
- Alexa Fluor® 700 Anti-CD24 antibody [SN3] (ab269310)
- FITC Anti-CD24 antibody [SN3] (ab30350)
- PE Anti-CD24 antibody [SN3], prediluted (ab30351)
- PE Anti-CD24 antibody [SN3] (ab77219)
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134375 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use a concentration of 1 - 12 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| Notes |
|---|
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Flow Cyt
Use a concentration of 1 - 12 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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Target
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Function
Modulates B-cell activation responses. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells. -
Tissue specificity
B-cells. Expressed in a number of B-cell lines including P32/SH and Namalwa. Expressed in erythroleukemia cell and small cell lung carcinoma cell lines. Also expressed on the surface of T-cells. -
Involvement in disease
Genetic variations in CD24 are associated with susceptibility to multiple sclerosis (MS) [MIM:126200]. A multifactorial, inflammatory, demyelinating disease of the central nervous system. Sclerotic lesions are characterized by perivascular infiltration of monocytes and lymphocytes and appear as indurated areas in pathologic specimens (sclerosis in plaques). The pathological mechanism is regarded as an autoimmune attack of the myelin sheat, mediated by both cellular and humoral immunity. Clinical manifestations include visual loss, extra-ocular movement disorders, paresthesias, loss of sensation, weakness, dysarthria, spasticity, ataxia and bladder dysfunction. Genetic and environmental factors influence susceptibility to the disease. Note=Polymorphisms in CD24 may act as a genetic modifier for susceptibility and progression of MS in some populations, perhaps by affecting the efficiency of CD24 expression on the cell surface. -
Sequence similarities
Belongs to the CD24 family. -
Post-translational
modificationsExtensively O-glycosylated. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 100133941 Human
- Omim: 600074 Human
- SwissProt: P25063 Human
- Unigene: 644105 Human
- Unigene: 721443 Human
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Alternative names
- CD 24 antibody
- CD24 antibody
- CD24 antigen (small cell lung carcinoma cluster 4 antigen) antibody
see all
Images
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Flow Cytometry - Anti-CD24 antibody [SN3] (ab134375)Flow cytometry analysis (surface staining) of human peripheral blood cells labeling CD24 with purified ab134375.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab134375 has been referenced in 6 publications.
- Yunusova NV et al. Metalloproteinases at the surface of small extrcellular vesicles in advanced ovarian cancer: Relationships with ascites volume and peritoneal canceromatosis index. Clin Chim Acta 494:116-122 (2019). PubMed: 30904547
- Tugutova EA et al. Relation between Tetraspanin- Associated and Tetraspanin- Non- Associated Exosomal Proteases and Metabolic Syndrome in Colorectal Cancer Patients Asian Pac J Cancer Prev 20:809-815 (2019). PubMed: 30909692
- Aghajani Nargesi A et al. Renal Artery Stenosis Alters Gene Expression in Swine Scattered Tubular-Like Cells. Int J Mol Sci 20:N/A (2019). PubMed: 31614781
- Nargesi AA et al. Renovascular disease induces mitochondrial damage in swine scattered tubular cells. Am J Physiol Renal Physiol 317:F1142-F1153 (2019). PubMed: 31461348
- Pavel M et al. Contact inhibition controls cell survival and proliferation via YAP/TAZ-autophagy axis. Nat Commun 9:2961 (2018). PubMed: 30054475
- Maliar A et al. Redirected T cells that target pancreatic adenocarcinoma antigens eliminate tumors and metastases in mice. Gastroenterology 143:1375-1384.e5 (2012). PubMed: 22819865