Overview

  • Product name

    Anti-CD24 antibody [SN3] (Phycoerythrin)
    See all CD24 primary antibodies
  • Description

    Mouse monoclonal [SN3] to CD24 (Phycoerythrin)
  • Host species

    Mouse
  • Conjugation

    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Glycoproteins purified from human NALM-1 cell line.

  • Positive control

    • Human blood cells.
  • General notes

    Purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.

Applications

Our Abpromise guarantee covers the use of ab77219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notes
    Flow Cyt: Use 20µl per 100µl of whole blood or 106 cells in suspension.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Modulates B-cell activation responses. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells.
    • Tissue specificity

      B-cells. Expressed in a number of B-cell lines including P32/SH and Namalwa. Expressed in erythroleukemia cell and small cell lung carcinoma cell lines. Also expressed on the surface of T-cells.
    • Involvement in disease

      Genetic variations in CD24 are associated with susceptibility to multiple sclerosis (MS) [MIM:126200]. A multifactorial, inflammatory, demyelinating disease of the central nervous system. Sclerotic lesions are characterized by perivascular infiltration of monocytes and lymphocytes and appear as indurated areas in pathologic specimens (sclerosis in plaques). The pathological mechanism is regarded as an autoimmune attack of the myelin sheat, mediated by both cellular and humoral immunity. Clinical manifestations include visual loss, extra-ocular movement disorders, paresthesias, loss of sensation, weakness, dysarthria, spasticity, ataxia and bladder dysfunction. Genetic and environmental factors influence susceptibility to the disease. Note=Polymorphisms in CD24 may act as a genetic modifier for susceptibility and progression of MS in some populations, perhaps by affecting the efficiency of CD24 expression on the cell surface.
    • Sequence similarities

      Belongs to the CD24 family.
    • Post-translational
      modifications

      Extensively O-glycosylated.
    • Cellular localization

      Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • CD 24 antibody
      • CD24 antibody
      • CD24 antigen (small cell lung carcinoma cluster 4 antigen) antibody
      • CD24 antigen antibody
      • CD24 molecule antibody
      • CD24_HUMAN antibody
      • CD24A antibody
      • FLJ22950 antibody
      • FLJ43543 antibody
      • GPI linked surface mucin antibody
      • Heat stable antigen antibody
      • HSA antibody
      • MGC75043 antibody
      • Nectadrin antibody
      • Signal transducer CD24 antibody
      • Small cell lung carcinoma cluster 4 antigen antibody
      see all

    Images

    • ab77219 staining human MCF7 cells by Flow Cytometry. The primary antibody was diluted 1/5 and incubated with sample for 30 minutes at 4°C. The purple profile shows negative control (mouse IgG) vs. green profile ab77219 (CD24).

      See Abreview

    References

    This product has been referenced in:

    • Quayle LA  et al. Chemotherapy resistance and stemness in mitotically quiescent human breast cancer cells identified by fluorescent dye retention. Clin Exp Metastasis 35:831-846 (2018). Read more (PubMed: 30377878) »
    • Peckys DB  et al. Liquid-phase electron microscopy of molecular drug response in breast cancer cells reveals irresponsive cell subpopulations related to lack of HER2 homodimers. Mol Biol Cell N/A:N/A (2017). Read more (PubMed: 28794264) »
    See all 9 Publications for this product

    Customer reviews and Q&As

    1-6 of 6 Abreviews or Q&A

    Answer

    Thank you for your enquiry regardingab77219 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.

    CD24 is a cell surface marker and it is a high change to damage th epitope during enzymatic digestion. Has the customer tried EDTA or scraping off the cells from the tissue culture dish instead of applying trypsin for harvesting the cells.

    It may be worth performing a quick ICC/IF staining to see if the antibody detects CD24 or accidentally damaged.

    I hope this will be useful for you. Should you still have any problem with this antibody after following these suggestions, then please do not hesitate to contact our Technical Department again.

    Read More

    Question
    Answer

    Thank you for contacting us.

    The antibody concentration is 30 ug/ml. Thus, 1 vial contains 30 ug.

    For any bulk order, please contact our bulk specialist at sales@abcam.com

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More
    Application
    Flow Cytometry
    Sample
    Human Cell (MCF-7)
    Specification
    MCF-7
    Preparation
    Cell harvesting/tissue preparation method: Cell dissociation buffer
    Sample buffer: PBS + 0.2% BSA + 0.1% Sodium azide
    Fixation
    Paraformaldehyde
    Permeabilization
    No
    Gating Strategy
    Live cells

    Abcam user community

    Verified customer

    Submitted May 24 2012

    Question
    Answer

    Thank you for contacting Abcam

    The antibody ab77219 is supplied at 18ug/ml and although the lab did not provide the total volume supplied, we do guarantee that there will be enough volume present to carry out 100 tests and if you happen to be short then we will send a replacement to make up the volume. Based on the notes on the website, it is recommended use 20µl per 100µl of whole blood or 106 cells in suspension for flow cytometry.

    Please let me know if there is anything else I can help you with.

    Read More

    Question
    Answer

    Code de remise : 123456789 Date d’expiration : JJ MM AAAA Nous sommes ravis que vous souhaitiez participer à notre offre et tester ab77219 chez le singe. Le code de réduction correspondant est 123456789. Ce code de réduction est utilisable sous 120 jours et pour l’achat d’un autre anticorps primaire. Vous devez dans un premier temps soumettre une Abreview avec vos résultats chez le singe en notant ce code dans la section « Additional notes ». Ce code sera ainsi activé et vous pourrez par la suite contacter notre service clientèle par téléphone pour passer votre prochaine commande, en vous munissant des informations suivantes : code de réduction, numéro de commande de ab77219. Nous restons à votre disposition pour plus d’informations concernant cette offre promotionnelle. Dans l’attente de recevoir votre Abreview nous vous souhaitons bonne chance dans vos recherches. Termes et Conditions : www.abcam.com/collaborationdiscount.

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    Answer

    Thanks for your reply and for the information. Ab77219 and ab81424 have been tested in flow cytometry and are guaranteed to work in FC with human cells. You are correct, they have not been tested in ICC/IF, and it is possible that they will work but we just don't have any information about this. Please let me know if you have any questions about these antibodies or if there is anything else that we can help with.

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