Anti-CD24 antibody [SN3] (Phycoerythrin) (ab77219)


  • Product name
    Anti-CD24 antibody [SN3] (Phycoerythrin)
    See all CD24 primary antibodies
  • Description
    Mouse monoclonal [SN3] to CD24 (Phycoerythrin)
  • Host species
  • Conjugation
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Glycoproteins purified from human NALM-1 cell line.

  • Positive control
    • Human blood cells.
  • General notes

    Purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.


Our Abpromise guarantee covers the use of ab77219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notes
    Flow Cyt: Use 20µl per 100µl of whole blood or 106 cells in suspension.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Modulates B-cell activation responses. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells.
    • Tissue specificity
      B-cells. Expressed in a number of B-cell lines including P32/SH and Namalwa. Expressed in erythroleukemia cell and small cell lung carcinoma cell lines. Also expressed on the surface of T-cells.
    • Involvement in disease
      Genetic variations in CD24 are associated with susceptibility to multiple sclerosis (MS) [MIM:126200]. A multifactorial, inflammatory, demyelinating disease of the central nervous system. Sclerotic lesions are characterized by perivascular infiltration of monocytes and lymphocytes and appear as indurated areas in pathologic specimens (sclerosis in plaques). The pathological mechanism is regarded as an autoimmune attack of the myelin sheat, mediated by both cellular and humoral immunity. Clinical manifestations include visual loss, extra-ocular movement disorders, paresthesias, loss of sensation, weakness, dysarthria, spasticity, ataxia and bladder dysfunction. Genetic and environmental factors influence susceptibility to the disease. Note=Polymorphisms in CD24 may act as a genetic modifier for susceptibility and progression of MS in some populations, perhaps by affecting the efficiency of CD24 expression on the cell surface.
    • Sequence similarities
      Belongs to the CD24 family.
    • Post-translational
      Extensively O-glycosylated.
    • Cellular localization
      Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD 24 antibody
      • CD24 antibody
      • CD24 antigen (small cell lung carcinoma cluster 4 antigen) antibody
      • CD24 antigen antibody
      • CD24 molecule antibody
      • CD24_HUMAN antibody
      • CD24A antibody
      • FLJ22950 antibody
      • FLJ43543 antibody
      • GPI linked surface mucin antibody
      • Heat stable antigen antibody
      • HSA antibody
      • MGC75043 antibody
      • Nectadrin antibody
      • Signal transducer CD24 antibody
      • Small cell lung carcinoma cluster 4 antigen antibody
      see all


    • ab77219 staining human MCF7 cells by Flow Cytometry. The primary antibody was diluted 1/5 and incubated with sample for 30 minutes at 4°C. The purple profile shows negative control (mouse IgG) vs. green profile ab77219 (CD24).

      See Abreview


    This product has been referenced in:
    • Peckys DB  et al. Liquid-phase electron microscopy of molecular drug response in breast cancer cells reveals irresponsive cell subpopulations related to lack of HER2 homodimers. Mol Biol Cell N/A:N/A (2017). Read more (PubMed: 28794264) »
    • Sagara A  et al. Intrinsic Resistance to 5-Fluorouracil in a Brain Metastatic Variant of Human Breast Cancer Cell Line, MDA-MB-231BR. PLoS One 11:e0164250 (2016). Read more (PubMed: 27723829) »

    See all 8 Publications for this product

    Customer reviews and Q&As

    Thank you for your enquiry regardingab77219 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.

    CD24 is a cell surface marker and it is a high cha...

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    Thank you for contacting us.

    The antibody concentration is 30 ug/ml. Thus, 1 vial contains 30 ug.

    For any bulk order, please contact our bulk specialist at

    I hope this information is helpful to you. Pleas...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Flow Cytometry
    Human Cell (MCF-7)
    Cell harvesting/tissue preparation method: Cell dissociation buffer
    Sample buffer: PBS + 0.2% BSA + 0.1% Sodium azide
    Gating Strategy
    Live cells

    Abcam user community

    Verified customer

    Submitted May 24 2012

    Thank you for contacting Abcam

    The antibody ab77219 is supplied at 18ug/ml and although the lab did not provide the total volume supplied, we do guarantee that there will be enough volume present to carry out 100 tests and if you happen to be...

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    Code de remise : 123456789 Date d’expiration : JJ MM AAAA Nous sommes ravis que vous souhaitiez participer à notre offre et tester ab77219 chez le singe. Le code de réduction correspondant est 123456789. Ce code de réduction est utilisable s...

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    Thanks for your reply and for the information. Ab77219 and ab81424 have been tested in flow cytometry and are guaranteed to work in FC with human cells. You are correct, they have not been tested in ICC/IF, and it is possible that they will wor...

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