Anti-CD25 antibody [PC61.5] (PerCP/Cy5.5®) (ab210336)

Overview

  • Product name

    Anti-CD25 antibody [PC61.5] (PerCP/Cy5.5®)
    See all CD25 primary antibodies
  • Description

    Rat monoclonal [PC61.5] to CD25 (PerCP/Cy5.5®)
  • Host species

    Rat
  • Conjugation

    PerCP/Cy5.5®. Ex: 482nm, Em: 690nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity


    Predicted to work with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control

    • C57Bl/6 splenocytes.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Store In the Dark.
  • Storage buffer

    pH: 7.2
    Preservative: 0.09% Sodium azide
    Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    ab210336 was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
  • Clonality

    Monoclonal
  • Clone number

    PC61.5
  • Isotype

    IgG1
  • Light chain type

    lambda

Applications

Our Abpromise guarantee covers the use of ab210336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

Suggested use 0.25 µg.

Target

  • Relevance

    The interleukin 2 (IL2) receptor alpha (IL2RA) (CD25) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble CD25 has been isolated and determined to result from extracellular proteolyisis.
  • Cellular localization

    Type I Cell Membrane
  • Database links

  • Alternative names

    • IL2R antibody
    • IL2RA antibody
    • Interleukin 2 Receptor alpha antibody
    • TCGFR antibody

Images

  • Flow Cytometric analysis of Mouse C57Bl/6 spleen cells labeling CD25 with FITC anti-Mouse CD4 antibody and ab210336 at 0.25 µg (right panel) or PerCP/Cy5.5® Rat IgG1 isotype control at 0.25 µg (left panel).

References

ab210336 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab210336.
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