Anti-CD25 antibody [PC61.5] (Phycoerythrin) (ab210334)

Overview

  • Product name

    Anti-CD25 antibody [PC61.5] (Phycoerythrin)
    See all CD25 primary antibodies
  • Description

    Rat monoclonal [PC61.5] to CD25 (Phycoerythrin)
  • Host species

    Rat
  • Conjugation

    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control

    • C57Bl/6 mouse spleen cells
  • General notes

    ab210334 is used as a marker for T cells, B cells and dendritic cell subsets. Expression of CD25, CD4 and the transcription factor Foxp3 is regarded as a phenotypic signature for Treg cells. As such, this antibody is widely used for depletion of Treg cells in vivo, as well as to distinguish Treg cells from naive or conventional T cells which are CD25-.

    The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Store In the Dark.
  • Storage buffer

    pH: 7.2
    Preservative: 0.09% Sodium azide
    Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    ab210334 was purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    PC61.5
  • Isotype

    IgG1
  • Light chain type

    lambda

Applications

Our Abpromise guarantee covers the use of ab210334 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

Use at 0.125 µg.

Target

  • Relevance

    The interleukin 2 (IL2) receptor alpha (IL2RA) (CD25) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble CD25 has been isolated and determined to result from extracellular proteolyisis.
  • Cellular localization

    Type I Cell Membrane
  • Database links

  • Alternative names

    • IL2R antibody
    • IL2RA antibody
    • Interleukin 2 Receptor alpha antibody
    • TCGFR antibody

Images

  • Flow cytometric analysis of mouse C57Bl/6 spleen cells stained with APC Anti-Mouse CD4 antibody and ab210334 at 0.125 µg (right panel), or PE Rat IgG1 isotype control 0.125 µg (left panel).

References

ab210334 has not yet been referenced specifically in any publications.

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