Overview

  • Product name
    Anti-CD26 antibody [11D7]
    See all CD26 primary antibodies
  • Description
    Mouse monoclonal [11D7] to CD26
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length Human CD26 produced in HEK293T cells (NP_001926)

  • Positive control
    • IHC-P: Liver tissue Flow Cyt: Hela cells WB: Transfected HEK293T cell lysate ICC/IF: Transfected COS7 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab114033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 88 kDa.
IHC-P 1/50.
Flow Cyt 1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/100.

Target

  • Function
    Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline.
  • Tissue specificity
    Expressed specifically in lymphatic vessels but not in blood vessels in the skin, small intestine, esophagus, ovary, breast and prostate glands. Not detected in lymphatic vessels in the lung, kidney, uterus, liver and stomach (at protein level). Expressed in the poorly differentiated crypt cells of the small intestine as well as in the mature villous cells. Expressed at very low levels in the colon.
  • Sequence similarities
    Belongs to the peptidase S9B family. DPPIV subfamily.
  • Domain
    The extracellular cysteine-rich region is necessary for association with collagen, dimer formation and optimal dipeptidyl peptidase activity.
  • Post-translational
    modifications
    The soluble form (Dipeptidyl peptidase 4 soluble form also named SDPP) derives from the membrane form (Dipeptidyl peptidase 4 membrane form also named MDPP) by proteolytic processing.
    N- and O-Glycosylated.
    Phosphorylated. Mannose 6-phosphate residues in the carbohydrate moiety are necessary for interaction with IGF2R in activated T-cells. Mannose 6-phosphorylation is induced during T-cell activation.
  • Cellular localization
    Cell membrane. Apical cell membrane. Cell projection > invadopodium membrane. Cell projection > lamellipodium membrane. Cell junction. Membrane raft. Translocated to the apical membrane through the concerted action of N- and O-Glycans and its association with lipid microdomains containing cholesterol and sphingolipids. Redistributed to membrane rafts in T-cell in a interleukin-12-dependent activation. Its interaction with CAV1 is necessary for its translocation to membrane rafts. Colocalized with PTPRC in membrane rafts. Colocalized with FAP in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with FAP on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma. Colocalized with ADA at the cell junction in lymphocyte-epithelial cell adhesion. Colocalized with IGF2R in internalized cytoplasmic vesicles adjacent to the cell surface and Secreted. Detected in the serum and the seminal fluid.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD26 antigen antibody
    • ADA-binding protein antibody
    • ADABP antibody
    • ADCP 2 antibody
    • ADCP-2 antibody
    • ADCP2 antibody
    • Adenosine deaminase complexing protein 2 antibody
    • CD 26 antibody
    • CD26 antibody
    • CD26 antigen 3 antibody
    • Dipeptidyl peptidase 4 antibody
    • Dipeptidyl peptidase 4 soluble form antibody
    • Dipeptidyl peptidase IV antibody
    • Dipeptidyl peptidase IV membrane form antibody
    • Dipeptidyl peptidase IV soluble form antibody
    • Dipeptidyl peptidase, intestinal antibody
    • Dipeptidylpeptidase 4 antibody
    • Dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2) antibody
    • Dipeptidylpeptidase IV antibody
    • DPP 4 antibody
    • DPP IV antibody
    • DPP IV estoenzyme antibody
    • DPP4 antibody
    • DPP4_HUMAN antibody
    • DPPIV antibody
    • Intestinal dipeptidyl peptidase antibody
    • T cell activation antigen CD26 antibody
    • T-cell activation antigen CD26 antibody
    • TP 103 antibody
    • TP103 antibody
    see all

Images

  • All lanes : Anti-CD26 antibody [11D7] (ab114033) at 1/500 dilution

    Lane 1 : HEK293T cells were transfected with the pCMV6-ENTRY control cDNA for 48 hrs and lysed
    Lane 2 : HEK293T cells were transfected with the pCMV6-ENTRY DPP4 cDNA for 48 hrs and lysed

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 88 kDa



    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC209466)
  • Immunohistochemical staining of paraffin embedded liver tissue using ab114033 at 1/50
  • Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DPP4, using ab114033 at 1/100
  • Flow cytometric analysis of Hela cells, using ab114033 at 1/100 (Red) compared to a nonspecific negative control antibody (Blue)

References

This product has been referenced in:
  • Song Y  et al. Expression of GLP-1 receptor and CD26 in human thyroid C-cells: The association of thyroid C-cell tumorigenesis with incretin-based medicine. Oncol Lett 13:2684-2690 (2017). IHC-P ; Human . Read more (PubMed: 28454451) »
  • Zheng B  et al. A three-gene panel that distinguishes benign from malignant thyroid nodules. Int J Cancer 136:1646-54 (2015). Read more (PubMed: 25175491) »
See all 3 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

En principio ab28340 detecta una banda a un peso algo superior al esperado debido a modificaciones post translacionales, el otro, ab114033 muestra una banda a un peso molecular menor que también aparece en la calle que contiene el control a mucha menor concentración.

No tenemos evidencia del origen de esa banda, es posible que se deba al procesado de CD26, pero no hay datos que lo confirmen.

Siento no poder ser de más ayuda en esta ocasión. Si necesitas algo mas, no dudes en consultarnos de nuevo.

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Answer

Gracias por contactarnos.

Llevando a cabo una búsqueda de anticuerpos contra las proteínas indicadas puedo sugerirte las siguientes alternativas:

Contra CD26: https://www.abcam.com/cd26-antibody-ab28340.html o https://www.abcam.com/cd26-antibody-11d7-ab114033.html. Ambos están testados, y por tanto garantizados en tejidos / células humanas mediante IHC-P y WB.

Para GLP1 habría una opción: https://www.abcam.com/glp1-antibody-4f3-ab23472.html que cumple con los requisitos que buscáis.

Te informo además que actualmente tenemos una promoción por la que al comprar cualquier anticuerpo primario os regalamos otro primario Rabbit Monoclonal. Simplemente indica el código RABMAB-XBSMG en el próximo pedido. Para más información picha https://www.abcam.com/index.html?pageconfig=resource&rid=15447.

Confío en haberte ayudado. En caso contrario no dudes en volvernos a contactar.

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Answer

Thank you for confirming this information and for your help and cooperation with this case.

As requested, I have asked our accounting department to issue you with a credit note. This can then be redeemed against the invoice of a future order.

Credit ID: #####

As usual if you have any further questions regarding this credit note, please contact the accounts department by email at creditcontrol@abcam.com. Please refer to the credit ID number in any correspondence with the accounting department.

I would like to wish the customer good luck with their research. The technical team is always at your service, should you require further expert advice.

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Question

Dear Tech Support Team,

Please read below the details of customer's complaint. The images are attached as well.

Thanks in advance for your assistance.

1) Abcam product code ab ab114033

2) Abcam order reference number or product batch number lot:GR72481-2

3) Description of the problem there were no specific bands

4) Sample preparation:

Type of sample (whole cell lysates, fraction, recombinant protein…):

Lysis buffer RIPA

Protease inhibitors: yes

Phosphatase inhibitors yes

Reducing agent DTT

Boiling for ≥5 min? yes /no

Protein loaded ug/lane or cells/lane 100ug

Positive control no

Negative control no

5) Percentage of gel 10%

Type of membrane nitrocellulose

Protein transfer verified by ponso

Blocking agent and concentration 5% milk in TBST

Blocking time 30 min

Blocking temperature RT


6) Primary antibody (If more than one was used, describe in additional notes) :

Concentration or dilution 1:500 as recommended in the data sheet

Diluent buffer 5% milk in TBST

Incubation time O.N

Incubation temperature: 4oC

7) Secondary antibody:

Species: Goat

Reacts against: Mouse

Concentration or dilution 1:5000

Diluent buffer 5% milk in TBST

Incubation time 1h

Incubation temperature: RT

Fluorochrome or enzyme conjugate: HPRT

8) Washing after primary and secondary antibodies:

Buffer TBST

Number of washes 4

9) Detection method ECL on film

10) How many times have you run this staining? 3

Do you obtain the same results every time? yes

What steps have you altered to try and optimize the use of this antibody? Washing after primary antibody with 5% milk in TBST

Document attachment: Attaching images of your blot is strongly recommended and can greatly speed up our investigation of your problem.

FIY: also in other applications, like IF and FACS, this antibody gave non specific staining

Best Regards,

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Answer

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

I would like to reassure you that ab114033 is tested and covered by our 6 month guarantee for use in WB and human samples. In the event that a product is not functioning in the applications and species cited on the product data sheet, we will be pleased to provide a credit note or free of charge replacement.

Reviewing this case, I would also appreciate if you can confirm some further details in order to help me to investigate for you:

1. Please provide the order number and date of purchase.

2. Please provide more sample details as requested on the form: Which cell line or tissue type was tested? From which species?

What samples were used in IF and FACS?

3. Is the current vial of secondary antibody working well with other primary antibodies? Has a no primary control been tried, what were the results?

4. Has the quality of the sample and loading been assessed using a loading control? What were the results? (We would usually recommend to load 20 - 30 ug of total protein per lane of the gel to ensure it is not overloaded).

Thank you for your cooperation. Please do not hesitate to contact me again with the further requested details.

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Answer

Vielen Dank für Ihre Anfrage. Ich kann Ihnen nun bestätigen, dass ab84384 (Anti-DBC1 antibody) eine Konzentration von ca. 1mg/ml hat. Außerdem kann ich Ihnen bestätigen, dass das Lot, das im Moment von ab114033 (Anti-CD26 antibody [11D7]) vorrätig ist, eine Konzentration von 0.66mg/ml aufweist. Beide Antikörper sind für IHC-P getestet und garantiert. Ich möchte Ihnen außerdem vorschlagen, einen Abreview zu verfassen und uns über Ihre Erfahrungen mit diesen Antikörpern zu berichten. Sie würden Abpoints verdienen, die Sie dann in einen Rabatt auf eine zukünftige Bestellung oder in einen Amazon Gutschein umtauschen können. Ich hoffe, diese Information ist hilfreich. Bitte zögern Sie nicht sich bei weiteren Fragen wieder an uns zu wenden.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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