Recombinant
RabMAb

Recombinant Anti-CD26 antibody [EPR22233-135] - BSA and Azide free (ab239376)

Overview

  • Product name

    Anti-CD26 antibody [EPR22233-135] - BSA and Azide free
    See all CD26 primary antibodies
  • Description

    Rabbit monoclonal [EPR22233-135] to CD26 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human CD26 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P27487

  • Positive control

    • FC: Human primary peripheral blood mononuclear cells.
  • General notes

    Ab239376 is the carrier-free version of ab229018. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab239376 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab239376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

Target

  • Function

    Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline.
  • Tissue specificity

    Expressed specifically in lymphatic vessels but not in blood vessels in the skin, small intestine, esophagus, ovary, breast and prostate glands. Not detected in lymphatic vessels in the lung, kidney, uterus, liver and stomach (at protein level). Expressed in the poorly differentiated crypt cells of the small intestine as well as in the mature villous cells. Expressed at very low levels in the colon.
  • Sequence similarities

    Belongs to the peptidase S9B family. DPPIV subfamily.
  • Domain

    The extracellular cysteine-rich region is necessary for association with collagen, dimer formation and optimal dipeptidyl peptidase activity.
  • Post-translational
    modifications

    The soluble form (Dipeptidyl peptidase 4 soluble form also named SDPP) derives from the membrane form (Dipeptidyl peptidase 4 membrane form also named MDPP) by proteolytic processing.
    N- and O-Glycosylated.
    Phosphorylated. Mannose 6-phosphate residues in the carbohydrate moiety are necessary for interaction with IGF2R in activated T-cells. Mannose 6-phosphorylation is induced during T-cell activation.
  • Cellular localization

    Cell membrane. Apical cell membrane. Cell projection > invadopodium membrane. Cell projection > lamellipodium membrane. Cell junction. Membrane raft. Translocated to the apical membrane through the concerted action of N- and O-Glycans and its association with lipid microdomains containing cholesterol and sphingolipids. Redistributed to membrane rafts in T-cell in a interleukin-12-dependent activation. Its interaction with CAV1 is necessary for its translocation to membrane rafts. Colocalized with PTPRC in membrane rafts. Colocalized with FAP in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with FAP on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma. Colocalized with ADA at the cell junction in lymphocyte-epithelial cell adhesion. Colocalized with IGF2R in internalized cytoplasmic vesicles adjacent to the cell surface and Secreted. Detected in the serum and the seminal fluid.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD26 antigen antibody
    • ADA-binding protein antibody
    • ADABP antibody
    • ADCP 2 antibody
    • ADCP-2 antibody
    • ADCP2 antibody
    • Adenosine deaminase complexing protein 2 antibody
    • CD 26 antibody
    • CD26 antibody
    • CD26 antigen 3 antibody
    • Dipeptidyl peptidase 4 antibody
    • Dipeptidyl peptidase 4 soluble form antibody
    • Dipeptidyl peptidase IV antibody
    • Dipeptidyl peptidase IV membrane form antibody
    • Dipeptidyl peptidase IV soluble form antibody
    • Dipeptidyl peptidase, intestinal antibody
    • Dipeptidylpeptidase 4 antibody
    • Dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2) antibody
    • Dipeptidylpeptidase IV antibody
    • DPP 4 antibody
    • DPP IV antibody
    • DPP IV estoenzyme antibody
    • DPP4 antibody
    • DPP4_HUMAN antibody
    • DPPIV antibody
    • Intestinal dipeptidyl peptidase antibody
    • T cell activation antigen CD26 antibody
    • T-cell activation antigen CD26 antibody
    • TP 103 antibody
    • TP103 antibody
    see all

Images

  • Flow cytometric analysis of human primary peripheral blood mononuclear cells (PBMC) labeling CD26 with ab229018 at 1/600 (right) compared with a isotype control Rabbit monoclonal IgG (ab172730) (left). Goat anti rabbit IgG Fc (Dylight® 488, ab98462), at 1/2000 dilution was used as the secondary antibody.

    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab229018).

References

ab239376 has not yet been referenced specifically in any publications.

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