Key features and details
- Rabbit polyclonal to CD27
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-CD27 antibody
See all CD27 primary antibodies
DescriptionRabbit polyclonal to CD27
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant full length protein within Human CD27 aa 20-191. The exact sequence is proprietary.
Database link: P26842
- WB: Raji, HeLa, Jurkat and HL-60 cell lysates. Mouse thymus tissue lysate. IHC: rat and mouse spleen tissues. IF: Jurkat cells, rat and mouse thymus tissues.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab175403 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
|WB||1/500 - 1/2000. Predicted molecular weight: 29 kDa.|
|IHC-P||1/50 - 1/200.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionReceptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.
Tissue specificityFound in most T-lymphocytes.
Sequence similaritiesContains 3 TNFR-Cys repeats.
modificationsPhosphorylated and O-glycosylated.
- Information by UniProt
- CD 27 antibody
- CD27 antibody
- CD27 antigen antibody
Paraffin-embedded mouse spleen tissue stained for CD27 using ab175403 at 1/100 dilution in immunohistochemical analysis.
Immunofluorescence staining of mouse thymus tissue stained for CD27 with ab175403 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).
All lanes : Anti-CD27 antibody (ab175403) at 1/3000 dilution
Lane 1 : Raji cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HL-60 cell lysate
Lane 5 : Mouse thymus tissue lysate
Lysates/proteins at 25 µg per lane.
All lanes : HRP Goat AntiRabbit IgG (H+L) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 29 kDa
Exposure time: 90 seconds
Blocking buffer: 3% nonfat dry milk in TBST.
Paraffin-embedded rat spleen tissue stained for CD27 using ab175403 at 1/100 dilution in immunohistochemical analysis.
Immunofluorescence staining of rat thymus tissue stained for CD27 with ab175403 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).
Immunofluorescence staining of Jurkat cells stained for CD27 with ab175403 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).
ab175403 has not yet been referenced specifically in any publications.