Overview

  • Product name

  • Description

    Goat polyclonal to CD27
  • Host species

    Goat
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human CD27 aa 161-175 (internal sequence) (Cysteine residue). (NP_001233.1).
    Sequence:

    C-QTLADFRQLPARTLS


    Database link: P26842

  • Positive control

    • WB: Human spleen, tonsil and thymus lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab223635 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 29 kDa).

Target

  • Function

    Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.
  • Tissue specificity

    Found in most T-lymphocytes.
  • Sequence similarities

    Contains 3 TNFR-Cys repeats.
  • Post-translational
    modifications

    Phosphorylated and O-glycosylated.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD 27 antibody
    • CD27 antibody
    • CD27 antigen antibody
    • CD27 molecule antibody
    • CD27_HUMAN antibody
    • CD27L receptor antibody
    • LPFS2 antibody
    • MGC20393 antibody
    • OTTHUMP00000238557 antibody
    • S152 antibody
    • T cell activation antigen CD27 antibody
    • T cell antivation antigen S152 antibody
    • T-cell activation antigen CD27 antibody
    • T14 antibody
    • TNFRSF 7 antibody
    • TNFRSF7 antibody
    • TNFSF7 antibody
    • Tp 55 antibody
    • Tp55 antibody
    • Tumor necrosis factor receptor superfamily member 7 antibody
    see all

Images

  • All lanes : Anti-CD27 antibody (ab223635) at 0.3 µg/ml

    Lane 1 : Human spleen lysate (in RIPA buffer)
    Lane 2 : Human tonsil lysate (in RIPA buffer)
    Lane 3 : Human thymus lysate (in RIPA buffer)

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 29 kDa
    Observed band size: 45 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

    The observed molecular weight corresponds to the glycosylated form.

References

ab223635 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab223635.
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