Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab223635 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab223635 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 29 kDa).|
FunctionReceptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.
Tissue specificityFound in most T-lymphocytes.
Sequence similaritiesContains 3 TNFR-Cys repeats.
modificationsPhosphorylated and O-glycosylated.
- Information by UniProt
- CD 27 antibody
- CD27 antibody
- CD27 antigen antibody
All lanes : Anti-CD27 antibody (ab223635) at 0.3 µg/ml
Lane 1 : Human spleen lysate (in RIPA buffer)
Lane 2 : Human tonsil lysate (in RIPA buffer)
Lane 3 : Human thymus lysate (in RIPA buffer)
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 29 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
The observed molecular weight corresponds to the glycosylated form.
ab223635 has not yet been referenced specifically in any publications.