Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR083G] to CD27
- Suitable for: WB, IP, IHC-P, ICC
- Reacts with: Human
Product nameAnti-CD27 antibody [BLR083G]
See all CD27 primary antibodies
DescriptionRabbit monoclonal [BLR083G] to CD27
Tested applicationsSuitable for: WB, IP, IHC-P, ICCmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human CD27 aa 21-187 (extracellular). NP_001233.1
TPAPKSCPERHYWAQGKLCCQMCEPGTFLVKDCDQHRKAAQCDPCIPGVS FSPDHHTRPHCESCRHCNSGLLVRNCTITANAECACRNGWQCRDKECTEC DPLPNPSLTARSSQALSPHPQPTHLPYVSEMLEARTAGHMQTLADFRQLP ARTLSTHWPPQRSLCSS
Database link: P26842
- IHC-P: Human tonsil tissue. ICC: Ramos cells. WB: Ramos, CCRF-CEM and Daudi whole cell lysate. IP: Ramos whole cell lysate.
This product is sold under License from Bethyl Laboratories, Inc
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purification notesPurified from TCS.
Our Abpromise guarantee covers the use of ab265589 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 29 kDa.|
|IP||Use at an assay dependent concentration.
20 µl/mg lysate.
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC||1/100 - 1/500.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with ICC staining protocol.
FunctionReceptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.
Tissue specificityFound in most T-lymphocytes.
Sequence similaritiesContains 3 TNFR-Cys repeats.
modificationsPhosphorylated and O-glycosylated.
- Information by UniProt
- CD 27 antibody
- CD27 antibody
- CD27 antigen antibody
All lanes : Anti-CD27 antibody [BLR083G] (ab265589) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : Ramos whole cell lysate
Lane 3 : HEK-293T whole cell lysate
Lane 4 : Raji whole cell lysate
Lane 5 : Jurkat whole cell lysate
Lane 6 : U-2 OS whole cell lysate
Lane 7 : Daudi whole cell lysate
Lane 8 : MCF7 whole cell lysate
Lane 9 : CCRF-CEM whole cell lysate
Lysates/proteins at 50 µg per lane.
All lanes : HRP conjugated goat anti-rabbit IgG
Predicted band size: 29 kDa
Exposure time: 3 minutes
Cells prepared using NETN lysis buffer.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling CD27 with ab265589 at 1/100 dilution. Detection: DAB.
Immunocytochemical analysis of formalin-fixed, paraffin-embedded ramos cells labeling CD27 with ab265589 at 1/100 dilution. Detection: DAB.
CD27 was immunoprecipitated from 1mg of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab265589 at 20 µl/mg lysate. Western blot was performed from the immunoprecipitate using ab265589 at 1/1000 dilution.
Lane 1: ab265589 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG.
Exposure time: 10 secs.
Cells prepared using NETN lysis buffer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab265589 has not yet been referenced specifically in any publications.