Recombinant Anti-CD27 antibody [EPR20585] (ab214043)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD27 antibody [EPR20585] (ab214043)](https://www.abcam.com/ps/products/214/ab214043/Images/ab214043-280629-anti-cd27-antibody-epr20585-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD27 antibody [EPR20585] (ab214043)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20585] to CD27
- Suitable for: IHC-P
- Reacts with: Mouse
Overview
-
Product name
Anti-CD27 antibody [EPR20585]
See all CD27 primary antibodies -
Description
Rabbit monoclonal [EPR20585] to CD27 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- IHC-P: Mouse spleen and thymus tissues.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20585 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab214043 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (1) |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
-
Function
Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1. -
Tissue specificity
Found in most T-lymphocytes. -
Sequence similarities
Contains 3 TNFR-Cys repeats. -
Post-translational
modificationsPhosphorylated and O-glycosylated. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 21940 Mouse
- SwissProt: P41272 Mouse
- Unigene: 367714 Mouse
-
Alternative names
- CD 27 antibody
- CD27 antibody
- CD27 antigen antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD27 antibody [EPR20585] (ab214043)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD27 with ab214043 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on T cells in periarterial lymphatic sheath of mouse spleen (PMID: 11782274, PMID: 11728341). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD27 antibody [EPR20585] (ab214043)](https://www.abcam.com/ps/products/214/ab214043/Images/ab214043-280628-anti-cd27-antibody-epr20585-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD27 antibody [EPR20585] (ab214043)Immunohistochemical analysis of paraffin-embedded mouse thymus tissue labeling CD27 with ab214043 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on mouse thymus (PMID: 11728341). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Anti-CD27 antibody [EPR20585] (ab214043)](https://www.abcam.com/ps/products/214/ab214043/Images/ab214043-3-benefits-of-recombinant-antibodies.png)
Anti-CD27 antibody [EPR20585] (ab214043)
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (0)
ab214043 has not yet been referenced specifically in any publications.