Recombinant
RabMAb

Recombinant Anti-CD27 antibody [EPR20585] - BSA and Azide free (ab236155)

Overview

  • Product name

    Anti-CD27 antibody [EPR20585] - BSA and Azide free
    See all CD27 primary antibodies
  • Description

    Rabbit monoclonal [EPR20585] to CD27 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant fragment within Mouse CD27 aa 1-200. The exact sequence is proprietary.
    Database link: P41272

  • Positive control

    • IHC-P: Mouse spleen tissue.
  • General notes

    Ab236155 is the carrier-free version of ab214043. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab236155 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab236155 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.
  • Tissue specificity

    Found in most T-lymphocytes.
  • Sequence similarities

    Contains 3 TNFR-Cys repeats.
  • Post-translational
    modifications

    Phosphorylated and O-glycosylated.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD 27 antibody
    • CD27 antibody
    • CD27 antigen antibody
    • CD27 molecule antibody
    • CD27_HUMAN antibody
    • CD27L receptor antibody
    • LPFS2 antibody
    • MGC20393 antibody
    • OTTHUMP00000238557 antibody
    • S152 antibody
    • T cell activation antigen CD27 antibody
    • T cell antivation antigen S152 antibody
    • T-cell activation antigen CD27 antibody
    • T14 antibody
    • TNFRSF 7 antibody
    • TNFRSF7 antibody
    • TNFSF7 antibody
    • Tp 55 antibody
    • Tp55 antibody
    • Tumor necrosis factor receptor superfamily member 7 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded mouse thymus tissue labeling CD27 with ab214043 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on mouse thymus (PMID: 11728341). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214043).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD27 with ab214043 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on T cells in periarterial lymphatic sheath of mouse spleen (PMID: 11782274, PMID: 11728341). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214043).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

ab236155 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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