Recombinant

Recombinant Anti-CD27 antibody [EPR21209] (ab213318)

Overview

  • Product name

    Anti-CD27 antibody [EPR21209]
    See all CD27 primary antibodies
  • Description

    Rabbit monoclonal [EPR21209] to CD27
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Baboon, Macaque monkey, Gorilla
  • Immunogen

    Recombinant fragment (His-tag) corresponding to Human CD27 aa 1-200.
    Database link: P26842

  • Positive control

    • Flow Cyt: Daudi cells IHC-P: FFPE human normal tonsil tissue sections.
  • General notes

    This product is an in vitro raised antibody. It was made using synthetic libraries and phage display technology.

    This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).

Properties

Applications

Our Abpromise guarantee covers the use of ab213318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.
  • Tissue specificity

    Found in most T-lymphocytes.
  • Sequence similarities

    Contains 3 TNFR-Cys repeats.
  • Post-translational
    modifications

    Phosphorylated and O-glycosylated.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD 27 antibody
    • CD27 antibody
    • CD27 antigen antibody
    • CD27 molecule antibody
    • CD27_HUMAN antibody
    • CD27L receptor antibody
    • LPFS2 antibody
    • MGC20393 antibody
    • OTTHUMP00000238557 antibody
    • S152 antibody
    • T cell activation antigen CD27 antibody
    • T cell antivation antigen S152 antibody
    • T-cell activation antigen CD27 antibody
    • T14 antibody
    • TNFRSF 7 antibody
    • TNFRSF7 antibody
    • TNFSF7 antibody
    • Tp 55 antibody
    • Tp55 antibody
    • Tumor necrosis factor receptor superfamily member 7 antibody
    see all

Images

  • Overlay histogram showing Daudi cells stained with ab213318 (red line).The cells were incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab213318, 1/10 dilution) for 30 min at  4°C. The secondary antibody (black line) used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150081) at 1/2000 dilution for 30 min at 4°C. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >30,000 total events were collected.

  • IHC image of CD27 staining in human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab213318, 1µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

     For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times

References

ab213318 has not yet been referenced specifically in any publications.

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