Recombinant
RabMAb

Recombinant Anti-CD27 antibody [EPR8568] (ab133761)

Overview

  • Product name

    Anti-CD27 antibody [EPR8568]
    See all CD27 primary antibodies
  • Description

    Rabbit monoclonal [EPR8568] to CD27
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to a region within the extracellular domain of Human CD27 (UniProt ID: P26842).

  • Positive control

    • Ramos cells and cell lysate
  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133761 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 29 kDa).
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.
    • Tissue specificity

      Found in most T-lymphocytes.
    • Sequence similarities

      Contains 3 TNFR-Cys repeats.
    • Post-translational
      modifications

      Phosphorylated and O-glycosylated.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • CD 27 antibody
      • CD27 antibody
      • CD27 antigen antibody
      • CD27 molecule antibody
      • CD27_HUMAN antibody
      • CD27L receptor antibody
      • LPFS2 antibody
      • MGC20393 antibody
      • OTTHUMP00000238557 antibody
      • S152 antibody
      • T cell activation antigen CD27 antibody
      • T cell antivation antigen S152 antibody
      • T-cell activation antigen CD27 antibody
      • T14 antibody
      • TNFRSF 7 antibody
      • TNFRSF7 antibody
      • TNFSF7 antibody
      • Tp 55 antibody
      • Tp55 antibody
      • Tumor necrosis factor receptor superfamily member 7 antibody
      see all

    Images

    • Anti-CD27 antibody [EPR8568] (ab133761) at 1/1000 dilution + Ramos cell lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size: 29 kDa

    • Immunofluorescent staining of CD27 in Ramos cells, using ab133761 at a 1/50 dilution.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab133761 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab133761.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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