Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8568] to CD27
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
Product nameAnti-CD27 antibody [EPR8568]
See all CD27 primary antibodies
DescriptionRabbit monoclonal [EPR8568] to CD27
Tested applicationsSuitable for: WB, ICC/IFmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Rat, Human
Synthetic peptide corresponding to a region within the extracellular domain of Human CD27 (UniProt ID: P26842).
- Ramos cells and cell lysate
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Dissociation constant (KD)KD = 7.60 x 10 -13 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133761 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 29 kDa).|
|ICC/IF||1/50 - 1/100.|
FunctionReceptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.
Tissue specificityFound in most T-lymphocytes.
Sequence similaritiesContains 3 TNFR-Cys repeats.
modificationsPhosphorylated and O-glycosylated.
- Information by UniProt
- CD 27 antibody
- CD27 antibody
- CD27 antigen antibody
Anti-CD27 antibody [EPR8568] (ab133761) at 1/1000 dilution + Ramos cell lysate at 10 µg
Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 29 kDa
Immunofluorescent staining of CD27 in Ramos cells, using ab133761 at a 1/50 dilution.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab133761 has not yet been referenced specifically in any publications.