Key features and details
- Armenian hamster monoclonal [LG.3A10] to CD27 - Low endotoxin, Azide free
- Suitable for: Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-CD27 antibody [LG.3A10] - Low endotoxin, Azide free
See all CD27 primary antibodies
DescriptionArmenian hamster monoclonal [LG.3A10] to CD27 - Low endotoxin, Azide free
Host speciesArmenian hamster
SpecificityWhen the monoclonal antibody LG.3A10 is crosslinked on purified T cells, proliferation is increased. In the absence of crosslinking, the antibody inhibits CD27-CD70 interaction.
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Mouse, Human
The details of the immunogen for this antibody are not available.
- BALB/c mouse splenocytes.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by the LAL assay.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Constituent: 100% PBS
Aqueous buffer, no sodium azide.
Concentration information loading...
PurityProtein G purified
Purification notesFunctional grade purified.
Our Abpromise guarantee covers the use of ab185776 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 0.25µg for 105-8 cells.
In a final volume of 100 μL.
ab18479 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionReceptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.
Tissue specificityFound in most T-lymphocytes.
Sequence similaritiesContains 3 TNFR-Cys repeats.
modificationsPhosphorylated and O-glycosylated.
- Information by UniProt
- CD 27 antibody
- CD27 antibody
- CD27 antigen antibody
ab185776 has not yet been referenced specifically in any publications.