Anti-CD27 antibody [LG.3A10] - Low endotoxin, Azide free (ab185776)

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Overview

  • Product name
    Anti-CD27 antibody [LG.3A10] - Low endotoxin, Azide free
    See all CD27 primary antibodies
  • Description
    Armenian Hamster monoclonal [LG.3A10] to CD27 - Low endotoxin, Azide free
  • Host species
    Armenian hamster
  • Specificity
    When the monoclonal antibody LG.3A10 is crosslinked on purified T cells, proliferation is increased. In the absence of crosslinking, the antibody inhibits CD27-CD70 interaction.
  • Tested applications
    Suitable for: IHC-Fr, Functional Studies, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control
    • BALB/c mouse splenocytes.
  • General notes

    Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by the LAL assay.

Properties

Applications

Our Abpromise guarantee covers the use of ab185776 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration.
Flow Cyt Use 0.25µg for 105-8 cells.

In a final volume of 100 μL.

ab18479 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.
  • Tissue specificity
    Found in most T-lymphocytes.
  • Sequence similarities
    Contains 3 TNFR-Cys repeats.
  • Post-translational
    modifications
    Phosphorylated and O-glycosylated.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
    • Alternative names
      • CD 27 antibody
      • CD27 antibody
      • CD27 antigen antibody
      • CD27 molecule antibody
      • CD27_HUMAN antibody
      • CD27L receptor antibody
      • LPFS2 antibody
      • MGC20393 antibody
      • OTTHUMP00000238557 antibody
      • S152 antibody
      • T cell activation antigen CD27 antibody
      • T cell antivation antigen S152 antibody
      • T-cell activation antigen CD27 antibody
      • T14 antibody
      • TNFRSF 7 antibody
      • TNFRSF7 antibody
      • TNFSF7 antibody
      • Tp 55 antibody
      • Tp55 antibody
      • Tumor necrosis factor receptor superfamily member 7 antibody
      see all

    Images

    • Flow Cytometry - Anti-CD27 antibody [LG.3A10] - Low endotoxin, Azide free (ab185776)
      Flow Cytometry - Anti-CD27 antibody [LG.3A10] - Low endotoxin, Azide free (ab185776)

      Flow cytometric analysis of BALB/c splenocytes with Anti-Human/Mouse CD45R (B220) FITC and 0.125 µg of ab185776 followed by Anti-Armenian Hamster IgG PE. Total viable cells were analyzed.

    References

    ab185776 has not yet been referenced specifically in any publications.

    Publishing research using ab185776? Please let us know so that we can cite the reference in this datasheet.

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