Anti-CD27 antibody [LG.3A10] - Low endotoxin, Azide free (ab185776)
![Flow Cytometry - Anti-CD27 antibody [LG.3A10] - Low endotoxin, Azide free (ab185776)](https://www.abcam.com/ps/products/185/ab185776/Images/ab185776-219641-anti-cd27-antibody-lg3a10-flow-cytometry.jpg "Flow Cytometry - Anti-CD27 antibody [LG.3A10] - Low endotoxin, Azide free (ab185776)")
Key features and details
- Armenian hamster monoclonal [LG.3A10] to CD27 - Low endotoxin, Azide free
- Suitable for: Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CD27 antibody [LG.3A10] - Low endotoxin, Azide free
See all CD27 primary antibodies -
Description
Armenian hamster monoclonal [LG.3A10] to CD27 - Low endotoxin, Azide free -
Host species
Armenian hamster -
Specificity
When the monoclonal antibody LG.3A10 is crosslinked on purified T cells, proliferation is increased. In the absence of crosslinking, the antibody inhibits CD27-CD70 interaction. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- BALB/c mouse splenocytes.
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General notes
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by the LAL assay.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Constituent: 100% PBS
Aqueous buffer, no sodium azide. -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Functional grade purified. -
Clonality
Monoclonal -
Clone number
LG.3A10 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab185776 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 0.25µg for 105-8 cells. In a final volume of 100 μL. ab18479 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1. -
Tissue specificity
Found in most T-lymphocytes. -
Sequence similarities
Contains 3 TNFR-Cys repeats. -
Post-translational
modificationsPhosphorylated and O-glycosylated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 939 Human
- Entrez Gene: 21940 Mouse
- Omim: 186711 Human
- SwissProt: P26842 Human
- SwissProt: P41272 Mouse
- Unigene: 355307 Human
- Unigene: 367714 Mouse
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Alternative names
- CD 27 antibody
- CD27 antibody
- CD27 antigen antibody
see all
Images
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Flow Cytometry - Anti-CD27 antibody [LG.3A10] - Low endotoxin, Azide free (ab185776)
Flow cytometric analysis of BALB/c splenocytes with Anti-Human/Mouse CD45R (B220) FITC and 0.125 µg of ab185776 followed by Anti-Armenian Hamster IgG PE. Total viable cells were analyzed.
Protocols
Datasheets and documents
References (0)
ab185776 has not yet been referenced specifically in any publications.
