Anti-CD27 antibody [LPFS2/1611] (ab268144)
Key features and details
- Mouse monoclonal [LPFS2/1611] to CD27
- Suitable for: Flow Cyt, IHC-P, Protein Array, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-CD27 antibody [LPFS2/1611]
See all CD27 primary antibodies -
Description
Mouse monoclonal [LPFS2/1611] to CD27 -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, IHC-P, Protein Array, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human CD27 aa 1-260. The exact sequence is proprietary.
Database link: P26842 -
Positive control
- ICC/IF: Ramos cells. IHC-P: Human tonsil, spleen and colon tissue. Flow Cyt: Ramos cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading...
-
Purity
Protein A/G purified -
Purification notes
Purified from bioreactor concentrate. -
Clonality
Monoclonal -
Clone number
LPFS2/1611 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab268144 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use 1-2µg for 106 cells.
|
|
IHC-P |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
Protein Array |
Use at an assay dependent concentration.
|
|
ICC/IF |
Use a concentration of 1 - 2 µg/ml.
|
Notes |
---|
Flow Cyt
Use 1-2µg for 106 cells. |
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Protein Array
Use at an assay dependent concentration. |
ICC/IF
Use a concentration of 1 - 2 µg/ml. |
Target
-
Function
Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1. -
Tissue specificity
Found in most T-lymphocytes. -
Sequence similarities
Contains 3 TNFR-Cys repeats. -
Post-translational
modificationsPhosphorylated and O-glycosylated. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 939 Human
- Omim: 186711 Human
- SwissProt: P26842 Human
- Unigene: 355307 Human
-
Alternative names
- CD 27 antibody
- CD27 antibody
- CD27 antigen antibody
see all
Images
-
Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD27 using ab268144 at 2 μg/ml in immunohistochemical analysis.
-
Immunocytochemistry/ Immunofluorescence staining of Ramos (Human Burkitt's lymphoma cell line) cells staind for CD27 using ab268144 at 2µg/ml followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.
-
Flow cytometric analysis of Ramos (Human Burkitt's lymphoma cell line) cells labeling CD27 using ab268144 at 2 μg/million cells followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
-
Protein Array containing more than 19,000 full-length human proteins using ab268144.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
-
Formalin-fixed, paraffin-embedded human spleen tissue stained for CD27 using ab268144 at 2 μg/ml in immunohistochemical analysis.
-
Formalin-fixed, paraffin-embedded human colon tissue stained for CD27 using ab268144 at 2 μg/ml in immunohistochemical analysis.
-
Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD27 using ab268144 at 2 μg/ml in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
References (0)
ab268144 has not yet been referenced specifically in any publications.