Key features and details
- Mouse monoclonal [7B8B4] to CD272/BTLA
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-CD272/BTLA antibody [7B8B4]
See all CD272/BTLA primary antibodies
DescriptionMouse monoclonal [7B8B4] to CD272/BTLA
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human CD272/BTLA aa 31-157. (Expressed in E.coli).
Database link: Q7Z6A9
- WB: Recombinant human CD272/BTLA (aa 31-157) protein; CD272/BTLA (aa 31-157)-hIgGFc transfected HEK-293 cell lysate. ICC/IF: HeLa cells.
This product was previously labelled as CD272
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Concentration information loading...
PurityProtein G purified
Purification notesPurified from tissue culture supernatant.
Our Abpromise guarantee covers the use of ab233733 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 33 kDa.|
|ICC/IF||1/100 - 1/500.|
FunctionLymphocyte inhibitory receptor which inhibits lymphocytes during immune response.
Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
modificationsPhosphorylated on Tyr residues by TNFRSF14 and by antigen receptors cross-linking, both inducing association with PTPN6 and PTPN11.
- Information by UniProt
- B and T lymphocyte associated protein antibody
- B and T lymphocyte attenuator antibody
- B and T lymphocyte associated antibody
Anti-CD272/BTLA antibody [7B8B4] (ab233733) at 1/500 dilution + Recombinant human CD272 (aa 31-157) protein
Predicted band size: 33 kDa
Expected MW is 41 kDa.
All lanes : Anti-CD272/BTLA antibody [7B8B4] (ab233733) at 1/500 dilution
Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : CD272/BTLA (aa 31-157)-hIgGFc transfected HEK-293 cell lysate
Predicted band size: 33 kDa
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CD272/BTLA (green) using ab233733 at 1/200 dilution in ICC/IF.
Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233733 has not yet been referenced specifically in any publications.