Overview

  • Product name

  • Description

    Rabbit polyclonal to CD272/BTLA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human CD272/BTLA (internal sequence). Peptide corresponds to 17 amino acids near the center. (NP_861445)
    Database link: Q7Z6A9

  • Positive control

    • Jurkat cell lyate and Human small intestine tissue.
  • General notes

     This product was previously labelled as CD272

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181406 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 33 kDa).
ICC/IF Use a concentration of 20 µg/ml.

Target

  • Function

    Lymphocyte inhibitory receptor which inhibits lymphocytes during immune response.
  • Sequence similarities

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications

    Phosphorylated on Tyr residues by TNFRSF14 and by antigen receptors cross-linking, both inducing association with PTPN6 and PTPN11.
    N-glycosylated.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • B and T lymphocyte associated protein antibody
    • B and T lymphocyte attenuator antibody
    • B and T lymphocyte associated antibody
    • B- and T-lymphocyte attenuator antibody
    • B- and T-lymphocyte-associated protein antibody
    • BTLA antibody
    • BTLA_HUMAN antibody
    • BTLA1 antibody
    • CD272 antibody
    • CD272 antigen antibody
    • FLJ16065 antibody
    • MGC129743 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human small intestine tissue, labeling CD272/BTLA using ab181406 at 2.5 μg/mL.

  • Lane 1 : Anti-CD272/BTLA antibody (ab181406) at 1 µg/ml
    Lane 2 : Anti-CD272/BTLA antibody (ab181406) at 2 µg/ml

    All lanes : Jurkat cell lysate

    Lysates/proteins at 15 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 33 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?

  • Immunofluorescence analysis of Human small intestine tissue, labeling CD272/BTLA using ab181406 at 20 μg/mL.

References

This product has been referenced in:

  • Lan X  et al. Increased BTLA and HVEM in gastric cancer are associated with progression and poor prognosis. Onco Targets Ther 10:919-926 (2017). Read more (PubMed: 28243127) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab181406.
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