Key features and details
- Rabbit polyclonal to CD272/BTLA
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CD272/BTLA antibody
See all CD272/BTLA primary antibodies
DescriptionRabbit polyclonal to CD272/BTLA
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human CD272/BTLA (internal sequence). Peptide corresponds to 17 amino acids near the center. (NP_861445)
Database link: Q7Z6A9
- Jurkat cell lyate and Human small intestine tissue.
This product was previously labelled as CD272
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab181406 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 33 kDa).|
|ICC/IF||Use a concentration of 20 µg/ml.|
FunctionLymphocyte inhibitory receptor which inhibits lymphocytes during immune response.
Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
modificationsPhosphorylated on Tyr residues by TNFRSF14 and by antigen receptors cross-linking, both inducing association with PTPN6 and PTPN11.
- Information by UniProt
- B and T lymphocyte associated protein antibody
- B and T lymphocyte attenuator antibody
- B and T lymphocyte associated antibody
Immunohistochemical analysis of paraffin-embedded Human small intestine tissue, labeling CD272/BTLA using ab181406 at 2.5 μg/mL.
Lane 1 : Anti-CD272/BTLA antibody (ab181406) at 1 µg/ml
Lane 2 : Anti-CD272/BTLA antibody (ab181406) at 2 µg/ml
All lanes : Jurkat cell lysate
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Predicted band size: 33 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Immunofluorescence analysis of Human small intestine tissue, labeling CD272/BTLA using ab181406 at 20 μg/mL.
ab181406 has been referenced in 1 publication.
- Lan X et al. Increased BTLA and HVEM in gastric cancer are associated with progression and poor prognosis. Onco Targets Ther 10:919-926 (2017). PubMed: 28243127