Key features and details
- Rabbit polyclonal to CD272/BTLA
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CD272/BTLA antibody
See all CD272/BTLA primary antibodies
DescriptionRabbit polyclonal to CD272/BTLA
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human CD272/BTLA aa 1-164.
Database link: NP_001078826
- WB: 293T, A431, H1299, HeLa, MOLT4 and Raji cell lysates IHC-P: MDAMB468 xenograft
This product was previously labelled as CD272
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab96560 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 33 kDa.|
|IHC-P||1/100 - 1/500.|
FunctionLymphocyte inhibitory receptor which inhibits lymphocytes during immune response.
Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
modificationsPhosphorylated on Tyr residues by TNFRSF14 and by antigen receptors cross-linking, both inducing association with PTPN6 and PTPN11.
- Information by UniProt
- B and T lymphocyte associated protein antibody
- B and T lymphocyte attenuator antibody
- B and T lymphocyte associated antibody
ab96560, at a 1/100 dilution, staining CD272/BTLA in paraffin embedded MDAMB468 xenograft by Immunohistochemical analysis.
All lanes : Anti-CD272/BTLA antibody (ab96560) at 1/1000 dilution
Lane 1 : MOLT4 whole cell lysates
Lane 2 : Raji whole cell lysates
Lysates/proteins at 30 µg per lane.
Predicted band size: 33 kDa
12% SDS PAGE
ab96560 has been referenced in 1 publication.
- Ramsay AG et al. Multiple inhibitory ligands induce impaired T-cell immunologic synapse function in chronic lymphocytic leukemia that can be blocked with lenalidomide: establishing a reversible immune evasion mechanism in human cancer. Blood 120:1412-21 (2012). IHC ; Human . PubMed: 22547582