Overview

  • Product name

  • Description

    Rabbit polyclonal to CD272/BTLA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human CD272/BTLA aa 1-164.
    Database link: NP_001078826

  • Positive control

    • WB: 293T, A431, H1299, HeLa, MOLT4 and Raji cell lysates IHC-P: MDAMB468 xenograft
  • General notes

     This product was previously labelled as CD272

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 33 kDa.
IHC-P 1/100 - 1/500.

Target

  • Function

    Lymphocyte inhibitory receptor which inhibits lymphocytes during immune response.
  • Sequence similarities

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications

    Phosphorylated on Tyr residues by TNFRSF14 and by antigen receptors cross-linking, both inducing association with PTPN6 and PTPN11.
    N-glycosylated.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • B and T lymphocyte associated protein antibody
    • B and T lymphocyte attenuator antibody
    • B and T lymphocyte associated antibody
    • B- and T-lymphocyte attenuator antibody
    • B- and T-lymphocyte-associated protein antibody
    • BTLA antibody
    • BTLA_HUMAN antibody
    • BTLA1 antibody
    • CD272 antibody
    • CD272 antigen antibody
    • FLJ16065 antibody
    • MGC129743 antibody
    see all

Images

  • All lanes : Anti-CD272/BTLA antibody (ab96560) at 1/1000 dilution

    Lane 1 : MOLT4 whole cell lysates
    Lane 2 : Raji whole cell lysates

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 33 kDa



    12% SDS PAGE
  • ab96560, at a 1/100 dilution, staining CD272/BTLA in paraffin embedded MDAMB468 xenograft by Immunohistochemical analysis.

References

This product has been referenced in:

  • Ramsay AG  et al. Multiple inhibitory ligands induce impaired T-cell immunologic synapse function in chronic lymphocytic leukemia that can be blocked with lenalidomide: establishing a reversible immune evasion mechanism in human cancer. Blood 120:1412-21 (2012). IHC ; Human . Read more (PubMed: 22547582) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab96560.
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