Product nameAnti-CD272/BTLA antibody [EPR20539]
See all CD272/BTLA primary antibodies
DescriptionRabbit monoclonal [EPR20539] to CD272/BTLA
Tested applicationsSuitable for: Flow Cyt, IP, WB, ICC/IFmore details
Species reactivityReacts with: Mouse
Recombinant fragment within Mouse CD272/BTLA aa 1-200. The exact sequence is proprietary.
Database link: Q7TSA3
- WB: A20 whole cell lysate; Mouse spleen and lymph node lysates. ICC/IF: A20 cells. Flow Cyt: A20 cells and mouse splenocytes. IP: Mouse spleen lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab212089 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 55-70 kDa (predicted molecular weight: 33 kDa).|
FunctionLymphocyte inhibitory receptor which inhibits lymphocytes during immune response.
Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
modificationsPhosphorylated on Tyr residues by TNFRSF14 and by antigen receptors cross-linking, both inducing association with PTPN6 and PTPN11.
- Information by UniProt
- B and T lymphocyte associated protein antibody
- B and T lymphocyte attenuator antibody
- B and T lymphocyte associated antibody
Lane 1 : Anti-CD272/BTLA antibody [EPR20539] (ab212089) at 1/10000 dilution
Lanes 2-3 : Anti-CD272/BTLA antibody [EPR20539] (ab212089) at 1/1000 dilution
Lane 1 : A20 (mouse reticulum sarcoma cell line) whole cell lysate
Lane 2 : Mouse spleen lysate
Lane 3 : Mouse lymph node lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 33 kDa
Observed band size: 55-70 kDa why is the actual band size different from the predicted?
Exposure time : Lane 1: 5 seconds; Lanes 2-3: 30 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
Immunofluorescent analysis of 100% methanol fixed A20 (mouse reticulum sarcoma cell line) cells labeling CD272/BTLA with ab212089 at 1/100 dilution, followed by by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and membranous staining on A20 cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
Flow cytometric analysis of mouse splenocytes labeling CD272/BTLA with ab212089 at 1/500 dilution (right) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (left). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
Mouse splenocytes stained with CD3 and ab212089. Total viable cells were gated for the FC image. T cells (CD3+, upper population) show a moderate positive signal while B cells (lower right population in right panel) show stronger positive signal. The expression pattern is also reported in literature (PMID: 19949073).
Flow cytometric analysis of A20 (mouse reticulum sarcoma cell line) cell line labeling CD272/BTLA with ab212089 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
Total viable cells were gated for the FC image.
CD272/BTLA was immunoprecipitated from 0.35 mg mouse spleen lysate with ab212089 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab212089 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution
Lane 1: Mouse spleen lysate 10 μg (Input).
Lane 2: ab212089 IP in mouse spleen lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab212089 in mouse spleen lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
ab212089 has not yet been referenced specifically in any publications.