Recombinant
RabMAb

Recombinant Anti-CD272/BTLA antibody [EPR22224-271] - BSA and Azide free (ab254287)

Overview

  • Product name

    Anti-CD272/BTLA antibody [EPR22224-271] - BSA and Azide free
    See all CD272/BTLA primary antibodies
  • Description

    Rabbit monoclonal [EPR22224-271] to CD272/BTLA - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF,IP or WB
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human CD272/BTLA aa 1-200. The exact sequence is proprietary.
    Database link: Q7Z6A9

  • Positive control

    • IHC-P: Human tonsil and lymphoma tissue.
  • General notes

    Ab254287 is the carrier-free version of ab230976. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab254287 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22224-271
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab254287 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IP or WB.
  • Target

    • Function

      Lymphocyte inhibitory receptor which inhibits lymphocytes during immune response.
    • Sequence similarities

      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Post-translational
      modifications

      Phosphorylated on Tyr residues by TNFRSF14 and by antigen receptors cross-linking, both inducing association with PTPN6 and PTPN11.
      N-glycosylated.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • B and T lymphocyte associated protein antibody
      • B and T lymphocyte attenuator antibody
      • B and T lymphocyte associated antibody
      • B- and T-lymphocyte attenuator antibody
      • B- and T-lymphocyte-associated protein antibody
      • BTLA antibody
      • BTLA_HUMAN antibody
      • BTLA1 antibody
      • CD272 antibody
      • CD272 antigen antibody
      • FLJ16065 antibody
      • MGC129743 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD272/BTLA with ab230976 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in lymphocytes of human tonsil (PMID:19762537) is observed. Counterstained with hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
      Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230976).
    • Immunohistochemical analysis of paraffin-embedded human lymphoma tissue labeling CD272/BTLA with ab230976 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous and cytoplasmic staining in human lymphoma (PMID:19762537) is observed. Counterstained with hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
      Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230976).

    References

    ab254287 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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