Anti-CD276 antibody [6A1] (ab105922)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [6A1] (ab105922)](https://www.abcam.com/ps/products/105/ab105922/Images/ab105922-118300-anti-cd276-antibody-6a1-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [6A1] (ab105922)")
Key features and details
- Mouse monoclonal [6A1] to CD276
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD276 antibody [6A1]
See all CD276 primary antibodies -
Description
Mouse monoclonal [6A1] to CD276 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human CD276 aa 30-130.
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Positive control
- WB: HEK293 cell lysate IHC-P: Human cervical cancer and ovarian cancer tissuesFlow Cyt: PC-3 cells
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General notes
This product was changed from ascites to supernatant. Lot no’s high than GR209552-21 are from Tissue Culture Supernatant
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
6A1 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab105922 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/200 - 1/1000. Predicted molecular weight: 57 kDa. | |
| IHC-P | 1/200 - 1/1000. | |
| Flow Cyt | 1/200 - 1/400. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling. -
Tissue specificity
Ubiquitous but not detectable in peripheral blood lymphocytes or granulocytes. Weakly expressed in resting monocytes. Expressed in dendritic cells derived from monocytes. Expressed in epithelial cells of sinonasal tissue. Expressed in extravillous trophoblast cells and Hofbauer cells of the first trimester placenta and term placenta. -
Sequence similarities
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 2 Ig-like V-type (immunoglobulin-like) domains. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 80381 Human
- Omim: 605715 Human
- SwissProt: Q5ZPR3 Human
- Unigene: 744915 Human
- Unigene: 77873 Human
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Alternative names
- 4Ig B7 H3 antibody
- 4Ig-B7-H3 antibody
- AU016588 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [6A1] (ab105922)ab105922, at a 1/200 dilution, staining CD276 in paraffin-embedded Human ovarian cancer tissues.
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![Western blot - Anti-CD276 antibody [6A1] (ab105922)](https://www.abcam.com/ps/products/105/ab105922/Images/ab105922-118302-anti-cd276-antibody-6a1-western-blot.jpg)
Western blot - Anti-CD276 antibody [6A1] (ab105922)All lanes : Anti-CD276 antibody [6A1] (ab105922) at 1/200 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : Transfected HEK293 cell lysate
Predicted band size: 57 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [6A1] (ab105922)](https://www.abcam.com/ps/products/105/ab105922/Images/ab105922-118301-anti-cd276-antibody-6a1-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [6A1] (ab105922)ab105922, at a 1/200 dilution, staining CD276 in paraffin-embedded Human cervical cancer tissues. -
![Flow Cytometry - Anti-CD276 antibody [6A1] (ab105922)](https://www.abcam.com/ps/products/105/ab105922/Images/ab105922-118299-anti-cd276-antibody-6a1-flow-cytometry.jpg)
Flow Cytometry - Anti-CD276 antibody [6A1] (ab105922)Flow cytometric analysis of PC-3 cells using ab105922 (green) and negative control (purple). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [6A1] (ab105922)](https://www.abcam.com/ps/products/105/ab105922/Images/ab105922-289423-anti-cd276-antibody-6a1-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [6A1] (ab105922)This image is courtesy of an anonymous Abreview.Formaldehyde-fixed, paraffin-embedded human tonsil tissue stained for CD276 using ab105922 at 1/800 dilution in immunohistochemical analysis.
Protocols
Datasheets and documents
References (6)
ab105922 has been referenced in 6 publications.
- Iida K et al. Prognostic impact of tumor-infiltrating CD276/Foxp3-positive lymphocytes and associated circulating cytokines in patients undergoing radical nephrectomy for localized renal cell carcinoma. Oncol Lett 17:4004-4010 (2019). PubMed: 30930997
- Zheng Y et al. High expression of B7-H2 or B7-H3 is associated with poor prognosis in hepatocellular carcinoma. Mol Med Rep 19:4315-4325 (2019). PubMed: 30942404
- Tang YC et al. Ginsenoside Rg3 targets cancer stem cells and tumor angiogenesis to inhibit colorectal cancer progression in vivo. Int J Oncol 52:127-138 (2018). PubMed: 29115601
- Xu ZL et al. B7-H3 promotes malignant progression of muscle-invasive bladder cancer. Oncol Rep 40:2722-2733 (2018). PubMed: 30132557
- Li Y et al. B7-H3 promotes the proliferation, migration and invasiveness of cervical cancer cells and is an indicator of poor prognosis. Oncol Rep 38:1043-1050 (2017). PubMed: 28627681
- Huang C et al. B7-H3, B7-H4, Foxp3 and IL-2 expression in cervical cancer: Associations with patient outcome and clinical significance. Oncol Rep 35:2183-90 (2016). PubMed: 26848032
