Anti-CD276 antibody [6A1] (ab105922)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-CD276 antibody [6A1]
See all CD276 primary antibodies -
DescriptionMouse monoclonal [6A1] to CD276
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Host speciesMouse
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Tested applicationsSuitable for: WB, IHC-P, ELISA, Flow Cytmore details
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Species reactivityReacts with: Human
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Immunogen
Purified recombinant fragment of Human CD276 expressed in E. Coli.
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Positive control
- WB: HEK293 cell lysate IHC-P: Human cervical cancer and ovarian cancer tissues FC: PC-3 cells
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General notes
This product was changed from ascites to supernatant. Lot no’s high than GR209552-21 are from Tissue Culture Supernatant
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
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Storage bufferPreservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
PurityProtein G purified
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Purification notesPurified from tissue culture supernatant.
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ClonalityMonoclonal
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Clone number6A1
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
Applications
Our Abpromise guarantee covers the use of ab105922 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/200 - 1/1000. Predicted molecular weight: 57 kDa. | |
| IHC-P | 1/200 - 1/1000. | |
| ELISA | 1/10000. | |
| Flow Cyt | 1/200 - 1/400. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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FunctionMay participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling.
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Tissue specificityUbiquitous but not detectable in peripheral blood lymphocytes or granulocytes. Weakly expressed in resting monocytes. Expressed in dendritic cells derived from monocytes. Expressed in epithelial cells of sinonasal tissue. Expressed in extravillous trophoblast cells and Hofbauer cells of the first trimester placenta and term placenta.
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Sequence similaritiesBelongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 2 Ig-like V-type (immunoglobulin-like) domains. -
Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 80381 Human
- Omim: 605715 Human
- SwissProt: Q5ZPR3 Human
- Unigene: 744915 Human
- Unigene: 77873 Human
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Alternative names
- 4Ig B7 H3 antibody
- 4Ig-B7-H3 antibody
- AU016588 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [6A1] (ab105922)](https://www.abcam.com/ps/products/105/ab105922/Images/CD276-Primary-antibodies-ab105922-5.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [6A1] (ab105922)ab105922, at a 1/200 dilution, staining CD276 in paraffin-embedded Human ovarian cancer tissues. -
![Western blot - Anti-CD276 antibody [6A1] (ab105922)](https://www.abcam.com/ps/products/105/ab105922/Images/CD276-Primary-antibodies-ab105922-1.jpg)
Western blot - Anti-CD276 antibody [6A1] (ab105922)All lanes : Anti-CD276 antibody [6A1] (ab105922) at 1/200 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : Transfected HEK293 cell lysate
Predicted band size: 57 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [6A1] (ab105922)](https://www.abcam.com/ps/products/105/ab105922/Images/CD276-Primary-antibodies-ab105922-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [6A1] (ab105922)ab105922, at a 1/200 dilution, staining CD276 in paraffin-embedded Human cervical cancer tissues. -
![Flow Cytometry - Anti-CD276 antibody [6A1] (ab105922)](https://www.abcam.com/ps/products/105/ab105922/Images/CD276-Primary-antibodies-ab105922-6.jpg)
Flow Cytometry - Anti-CD276 antibody [6A1] (ab105922)Flow cytometric analysis of PC-3 cells using ab105922 (green) and negative control (purple). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [6A1] (ab105922)](https://www.abcam.com/ps/products/105/ab105922/Images/ab105922-289423-ab105922A.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [6A1] (ab105922)This image is courtesy of an anonymous Abreview.Formaldehyde-fixed, paraffin-embedded human tonsil tissue stained for CD276 using ab105922 at 1/800 dilution in immunohistochemical analysis.
Protocols
References
This product has been referenced in:
- Tang YC et al. Ginsenoside Rg3 targets cancer stem cells and tumor angiogenesis to inhibit colorectal cancer progression in vivo. Int J Oncol 52:127-138 (2018). Read more (PubMed: 29115601) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"