Anti-CD276 antibody [6A1] (ab105922)
Key features and details
- Mouse monoclonal [6A1] to CD276
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD276 antibody [6A1]
See all CD276 primary antibodies -
Description
Mouse monoclonal [6A1] to CD276 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human CD276 aa 30-130.
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Positive control
- WB: HEK293 cell lysate IHC-P: Human cervical cancer and ovarian cancer tissues.
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General notes
This product was changed from ascites to supernatant. Lot no’s high than GR209552-21 are from Tissue Culture Supernatant
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
6A1 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab105922 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/200 - 1/1000. Predicted molecular weight: 57 kDa.
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IHC-P | (3) |
1/200 - 1/1000.
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Notes |
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WB
1/200 - 1/1000. Predicted molecular weight: 57 kDa. |
IHC-P
1/200 - 1/1000. |
Target
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Function
May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling. -
Tissue specificity
Ubiquitous but not detectable in peripheral blood lymphocytes or granulocytes. Weakly expressed in resting monocytes. Expressed in dendritic cells derived from monocytes. Expressed in epithelial cells of sinonasal tissue. Expressed in extravillous trophoblast cells and Hofbauer cells of the first trimester placenta and term placenta. -
Sequence similarities
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 2 Ig-like V-type (immunoglobulin-like) domains. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 80381 Human
- Omim: 605715 Human
- SwissProt: Q5ZPR3 Human
- Unigene: 77873 Human
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Alternative names
- 4Ig B7 H3 antibody
- 4Ig-B7-H3 antibody
- AU016588 antibody
see all
Images
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ab105922, at a 1/200 dilution, staining CD276 in paraffin-embedded Human ovarian cancer tissues.
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All lanes : Anti-CD276 antibody [6A1] (ab105922) at 1/200 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : Transfected HEK293 cell lysate
Predicted band size: 57 kDa -
ab105922, at a 1/200 dilution, staining CD276 in paraffin-embedded Human cervical cancer tissues.
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Formaldehyde-fixed, paraffin-embedded human tonsil tissue stained for CD276 using ab105922 at 1/800 dilution in immunohistochemical analysis.
Protocols
Datasheets and documents
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Datasheet download
References (11)
ab105922 has been referenced in 11 publications.
- Lv C et al. Novel immune scoring dynamic nomograms based on B7-H3, B7-H4, and HHLA2: Potential prediction in survival and immunotherapeutic efficacy for gallbladder cancer. Front Immunol 13:984172 (2022). PubMed: 36159808
- Aicher WK et al. Expression patterns of the immune checkpoint ligand CD276 in urothelial carcinoma. BMC Urol 21:60 (2021). PubMed: 33845814
- Li H et al. MAPK-RAP1A Signaling Enriched in Hepatocellular Carcinoma Is Associated With Favorable Tumor-Infiltrating Immune Cells and Clinical Prognosis. Front Oncol 11:649980 (2021). PubMed: 34178637
- Muranushi R et al. High membrane expression of CMTM6 in hepatocellular carcinoma is associated with tumor recurrence. Cancer Sci 112:3314-3323 (2021). PubMed: 34080242
- Deng J et al. Expression and Clinical Significance of Immune Checkpoint Regulator B7-H3 (CD276) in Human Meningioma. World Neurosurg 135:e12-e18 (2020). PubMed: 31629137