Overview

  • Product name

    Anti-CD276 antibody [6A1]
    See all CD276 primary antibodies
  • Description

    Mouse monoclonal [6A1] to CD276
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, ELISA, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human CD276 aa 30-130.

  • Positive control

    • WB: HEK293 cell lysate IHC-P: Human cervical cancer and ovarian cancer tissues FC: PC-3 cells
  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR209552-21 are from Tissue Culture Supernatant

Properties

Applications

Our Abpromise guarantee covers the use of ab105922 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/1000. Predicted molecular weight: 57 kDa.
IHC-P 1/200 - 1/1000.
ELISA 1/10000.
Flow Cyt 1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function

    May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling.
  • Tissue specificity

    Ubiquitous but not detectable in peripheral blood lymphocytes or granulocytes. Weakly expressed in resting monocytes. Expressed in dendritic cells derived from monocytes. Expressed in epithelial cells of sinonasal tissue. Expressed in extravillous trophoblast cells and Hofbauer cells of the first trimester placenta and term placenta.
  • Sequence similarities

    Belongs to the immunoglobulin superfamily. BTN/MOG family.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 2 Ig-like V-type (immunoglobulin-like) domains.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 4Ig B7 H3 antibody
    • 4Ig-B7-H3 antibody
    • AU016588 antibody
    • B7 H3 antibody
    • B7 homolog 3 antibody
    • B7-H3 antibody
    • B7H3 antibody
    • B7RP-2 antibody
    • CD_antigen=CD276 antibody
    • CD276 antibody
    • CD276 antigen antibody
    • CD276 molecule antibody
    • CD276_HUMAN antibody
    • Costimulatory molecule antibody
    • Flags: Precursor antibody
    • PSEC0249 antibody
    • UNQ309/PRO352 antibody
    see all

Images

  • All lanes : Anti-CD276 antibody [6A1] (ab105922) at 1/200 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : Transfected HEK293 cell lysate

    Predicted band size: 57 kDa

  • ab105922, at a 1/200 dilution, staining CD276 in paraffin-embedded Human ovarian cancer tissues.
  • ab105922, at a 1/200 dilution, staining CD276 in paraffin-embedded Human cervical cancer tissues.
  • Flow cytometric analysis of PC-3 cells using ab105922 (green) and negative control (purple).
  • Formaldehyde-fixed, paraffin-embedded human tonsil tissue stained for CD276 using ab105922 at 1/800 dilution in immunohistochemical analysis.

    See Abreview

References

This product has been referenced in:

  • Iida K  et al. Prognostic impact of tumor-infiltrating CD276/Foxp3-positive lymphocytes and associated circulating cytokines in patients undergoing radical nephrectomy for localized renal cell carcinoma. Oncol Lett 17:4004-4010 (2019). Read more (PubMed: 30930997) »
  • Zheng Y  et al. High expression of B7-H2 or B7-H3 is associated with poor prognosis in hepatocellular carcinoma. Mol Med Rep 19:4315-4325 (2019). Read more (PubMed: 30942404) »
See all 4 Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Tonsil)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Ventana CC1 (pH8.5)
Permeabilization
No
Specification
Tonsil
Blocking step
Ventana blocking agent as blocking agent for 4 minute(s) · Concentration: 100% · Temperature: 36°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Feb 17 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Prostate cancer)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6.0
Permeabilization
No
Specification
Prostate cancer
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Nov 13 2015

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