Overview

  • Product name

  • Description

    Rabbit polyclonal to CD276
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, IP, IHC-Pmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human CD276 aa 484-534. The exact sequence is proprietary. (NP_001019907.1).
    Database link: Q5ZPR3

  • Positive control

    • IP: HeLa whole cell lysate (ab150035). IHC-P: Human prostate carconoma tissue ICC: MCF7 cells; CD276 transfected HEK-293 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab226256 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/5000.
IP Use at 2-10 µg/mg of lysate.
IHC-P 1/2000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling.
    • Tissue specificity

      Ubiquitous but not detectable in peripheral blood lymphocytes or granulocytes. Weakly expressed in resting monocytes. Expressed in dendritic cells derived from monocytes. Expressed in epithelial cells of sinonasal tissue. Expressed in extravillous trophoblast cells and Hofbauer cells of the first trimester placenta and term placenta.
    • Sequence similarities

      Belongs to the immunoglobulin superfamily. BTN/MOG family.
      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 2 Ig-like V-type (immunoglobulin-like) domains.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • 4Ig B7 H3 antibody
      • 4Ig-B7-H3 antibody
      • AU016588 antibody
      • B7 H3 antibody
      • B7 homolog 3 antibody
      • B7-H3 antibody
      • B7H3 antibody
      • B7RP-2 antibody
      • CD_antigen=CD276 antibody
      • CD276 antibody
      • CD276 antigen antibody
      • CD276 molecule antibody
      • CD276_HUMAN antibody
      • Costimulatory molecule antibody
      • Flags: Precursor antibody
      • PSEC0249 antibody
      • UNQ309/PRO352 antibody
      see all

    Images

    • Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for CD276 using ab226256 at 1/5000 dilution in immunohistochemical analysis.

      Detection: DAB staining. Counterstain: Hematoxylin.

    • CD276 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using NETN buffer; 1 mg for IP, 20% of IP loaded) with ab226256 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using a different rabbit anti-CD276 antibody at 1 µg/ml.

      Lane 1: ab226256 IP in HeLa whole cell lysate.

      Lane 2: Control IgG IP in HeLa whole cell lysate.

      Detection: Chemiluminescence with exposure time of 3 minutes.

    • MCF7 (human breast adenocarcinoma cell line) (left panel) and CD276 transfected HEK-293 (human epithelial cell line from embryonic kidney) (right panel) cells stained for CD276 using ab226256 at 1/5000 dilution in immunohistochemical analysis.

      Detection: DAB staining. 

    References

    ab226256 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Flow Cytometry
    Sample
    Mouse Cell (HM1 (mouse ovarian cancer cell line))
    Permeabilization
    Yes - BD Cytofix/Cytoperm Fixation/Permeabilization Solution kit Cat No. 554714
    Specification
    HM1 (mouse ovarian cancer cell line)
    Preparation
    Cell harvesting/tissue preparation method: MEMa with 10% FBS + antibiotics
    Sample buffer: PBS with 2% FBS
    Fixation
    BD Cytofix/Cytoperm Fixation/Permeabilization Solution kit Cat No. 554714

    泰斗 宮本

    Verified customer

    Submitted May 21 2019

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