Anti-CD276 antibody (ab226256)
Key features and details
- Rabbit polyclonal to CD276
- Suitable for: ICC, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-CD276 antibody
See all CD276 primary antibodies -
Description
Rabbit polyclonal to CD276 -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human CD276 aa 484-534. The exact sequence is proprietary. (NP_001019907.1).
Database link: Q5ZPR3 -
Positive control
- IP: HeLa whole cell lysate (ab150035). IHC-P: Human prostate carconoma tissue ICC: MCF7 cells; CD276 transfected HEK-293 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab226256 was affinity purified using an epitope specific to CD276 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab226256 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
1/5000.
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IP |
Use at 2-10 µg/mg of lysate.
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IHC-P |
1/2000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC
1/5000. |
IP
Use at 2-10 µg/mg of lysate. |
IHC-P
1/2000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling. -
Tissue specificity
Ubiquitous but not detectable in peripheral blood lymphocytes or granulocytes. Weakly expressed in resting monocytes. Expressed in dendritic cells derived from monocytes. Expressed in epithelial cells of sinonasal tissue. Expressed in extravillous trophoblast cells and Hofbauer cells of the first trimester placenta and term placenta. -
Sequence similarities
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 2 Ig-like V-type (immunoglobulin-like) domains. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 80381 Human
- Entrez Gene: 102657 Mouse
- Entrez Gene: 315716 Rat
- Omim: 605715 Human
- SwissProt: Q5ZPR3 Human
- SwissProt: Q8VE98 Mouse
- SwissProt: Q7TPB4 Rat
- Unigene: 77873 Human
see all -
Alternative names
- 4Ig B7 H3 antibody
- 4Ig-B7-H3 antibody
- AU016588 antibody
see all
Images
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Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for CD276 using ab226256 at 1/5000 dilution in immunohistochemical analysis.
Detection: DAB staining. Counterstain: Hematoxylin.
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CD276 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using NETN buffer; 1 mg for IP, 20% of IP loaded) with ab226256 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using a different rabbit anti-CD276 antibody at 1 µg/ml.
Lane 1: ab226256 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
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MCF7 (human breast adenocarcinoma cell line) (left panel) and CD276 transfected HEK-293 (human epithelial cell line from embryonic kidney) (right panel) cells stained for CD276 using ab226256 at 1/5000 dilution in immunohistochemical analysis.
Detection: DAB staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab226256 has been referenced in 4 publications.
- Zhu Y et al. Prognostic values of B7-H3, B7-H4, and HHLA2 expression in human pancreatic cancer tissues based on mIHC and spatial distribution analysis. Pathol Res Pract 234:153911 (2022). PubMed: 35489125
- Geng R et al. CD24: A Marker for an Extended Expansion Potential of Urothelial Cancer Cell Organoids In Vitro? Int J Mol Sci 23:N/A (2022). PubMed: 35628262
- Masemann D et al. Synergistic anti-tumor efficacy of oncolytic influenza viruses and B7-H3 immune- checkpoint inhibitors against IC-resistant lung cancers. Oncoimmunology 10:1885778 (2021). PubMed: 33643696
- Aicher WK et al. Expression patterns of the immune checkpoint ligand CD276 in urothelial carcinoma. BMC Urol 21:60 (2021). PubMed: 33845814