Key features and details
- Rabbit polyclonal to CD276
- Suitable for: ICC, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CD276 antibody
See all CD276 primary antibodies
DescriptionRabbit polyclonal to CD276
Tested applicationsSuitable for: ICC, IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human CD276 aa 484-534. The exact sequence is proprietary. (NP_001019907.1).
Database link: Q5ZPR3
- IP: HeLa whole cell lysate (ab150035). IHC-P: Human prostate carconoma tissue ICC: MCF7 cells; CD276 transfected HEK-293 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226256 was affinity purified using an epitope specific to CD276 immobilized on solid support.
Our Abpromise guarantee covers the use of ab226256 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|IHC-P||1/2000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionMay participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling.
Tissue specificityUbiquitous but not detectable in peripheral blood lymphocytes or granulocytes. Weakly expressed in resting monocytes. Expressed in dendritic cells derived from monocytes. Expressed in epithelial cells of sinonasal tissue. Expressed in extravillous trophoblast cells and Hofbauer cells of the first trimester placenta and term placenta.
Sequence similaritiesBelongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 2 Ig-like V-type (immunoglobulin-like) domains.
- Information by UniProt
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Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for CD276 using ab226256 at 1/5000 dilution in immunohistochemical analysis.
Detection: DAB staining. Counterstain: Hematoxylin.
CD276 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using NETN buffer; 1 mg for IP, 20% of IP loaded) with ab226256 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using a different rabbit anti-CD276 antibody at 1 µg/ml.
Lane 1: ab226256 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
MCF7 (human breast adenocarcinoma cell line) (left panel) and CD276 transfected HEK-293 (human epithelial cell line from embryonic kidney) (right panel) cells stained for CD276 using ab226256 at 1/5000 dilution in immunohistochemical analysis.
Detection: DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226256 has not yet been referenced specifically in any publications.