Recombinant Anti-CD276 antibody [SP206], prediluted (ab228178)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP206] to CD276, prediluted
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CD276 antibody [SP206], prediluted
See all CD276 primary antibodies -
Description
Rabbit monoclonal [SP206] to CD276, prediluted -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human tonsil, testis, stomach adenocarcinoma, lung, lung adenocarcinoma, lung SCC, colon, colon adenocarcinoma, breast, breast ductal carcinoma and stomach tissue. WB: HeLa, HEK293T and LNCaP cell lysates.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: Tris buffered saline, 1% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP206 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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KO cell pellets
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab228178 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/1.
Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Pre-diluted / ready to use. |
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WB |
Use at an assay dependent concentration.
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Notes |
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IHC-P
1/1. Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Pre-diluted / ready to use. |
WB
Use at an assay dependent concentration. |
Target
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Function
May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling. -
Tissue specificity
Ubiquitous but not detectable in peripheral blood lymphocytes or granulocytes. Weakly expressed in resting monocytes. Expressed in dendritic cells derived from monocytes. Expressed in epithelial cells of sinonasal tissue. Expressed in extravillous trophoblast cells and Hofbauer cells of the first trimester placenta and term placenta. -
Sequence similarities
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 2 Ig-like V-type (immunoglobulin-like) domains. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 80381 Human
- Entrez Gene: 102657 Mouse
- Entrez Gene: 315716 Rat
- Omim: 605715 Human
- SwissProt: Q5ZPR3 Human
- SwissProt: Q8VE98 Mouse
- SwissProt: Q7TPB4 Rat
- Unigene: 77873 Human
see all -
Alternative names
- 4Ig B7 H3 antibody
- 4Ig-B7-H3 antibody
- AU016588 antibody
see all
Images
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All lanes : Anti-CD276 antibody [SP206] (ab227670) at 1/400 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : CD276 knockout HeLa cell lysate
Lane 3 : LNCaP cell lysate
Lane 4 : Raji cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Observed band size: 90-110 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab227670).
Lanes 1- 4: Merged signal (red and green). Green - ab227670 observed at 90-110 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab227670 was shown to react with CD276 antigen in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab261756 (knockout cell lysate ab257098) was used. Wild-type HeLa and CD276 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab227670 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 400 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178)
Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for CD276 with ab228178 in immunohistochemical analysis.
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All lanes : Anti-CD276 antibody [SP206] (ab227670) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : CD276 knockout HEK293T cell lysate
Lane 3 : LNCaP cell lysate
Lane 4 : Raji cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Observed band size: 90-110 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab227670).
Lanes 1-4: Merged signal (red and green). Green - ab227670 observed at 90-110 kDa. Red - loading control ab8245 observed at 36 kDa.
ab227670 Anti-CD276 antibody [SP206] was shown to specifically react with CD276 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266658 (knockout cell lysate ab257097) was used. Wild-type and CD276 knockout samples were subjected to SDS-PAGE. ab227670 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178)
Formalin-fixed, paraffin-embedded human stomach tissue stained for CD276 with ab228178 in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178)
Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for CD276 with ab228178 in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178)
Formalin-fixed, paraffin-embedded human breast tissue stained for CD276 with ab228178 in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178)
Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for CD276 with ab228178 in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178)
Formalin-fixed, paraffin-embedded human colon tissue stained for CD276 with ab228178 in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178)
Formalin-fixed, paraffin-embedded human lung SCC tissue stained for CD276 with ab228178 in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178)
Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for CD276 with ab228178 in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178)
Formalin-fixed, paraffin-embedded human lung tissue stained for CD276 with ab228178 in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178)
Formalin-fixed, paraffin-embedded human testis tissue stained for CD276 with ab228178 in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178)
Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD276 with ab228178 in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab228178 has not yet been referenced specifically in any publications.