Recombinant Anti-CD276 antibody [SP206], prediluted KO Tested (ab228178)

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Western blot - Anti-CD276 antibody [SP206], prediluted (ab228178)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [SP206] to CD276, prediluted
Suitable for: IHC-P, WB
Knockout validated
Reacts with: Human

Carrier Free

Product name

Anti-CD276 antibody [SP206], prediluted
See all CD276 primary antibodies
Description

Rabbit monoclonal [SP206] to CD276, prediluted
Host species

Rabbit
Tested applications

Suitable for: IHC-P, WBmore details
Species reactivity

Reacts with: Human
Predicted to work with: Mouse, Rat
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Human tonsil, testis, stomach adenocarcinoma, lung, lung adenocarcinoma, lung SCC, colon, colon adenocarcinoma, breast, breast ductal carcinoma and stomach tissue. WB: HeLa, HEK293T and LNCaP cell lysates.
General notes

This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: Tris buffered saline, 1% BSA
Concentration information loading...
Purity

Protein A/G purified
Purification notes

Purified from TCS by protein A/G.
Clonality

Monoclonal
Clone number

SP206
Isotype

IgG
Research areas
- Neuroscience
- Processes

Alternative Versions
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
KO cell lines
- Human CD276 knockout HeLa cell line (ab261756)
- Human CD276 knockout HEK-293T cell line (ab266658)
KO cell lysates
- Human CD276 knockout HEK-293T cell lysate (ab257097)
- Human CD276 knockout HeLa cell lysate (ab257098)
KO cell pellets
- Human CD276 knockout HEK-293T cell pellet (ab278909)
Recombinant Protein
- Recombinant human CD276 protein (Fc Chimera Active) (ab216204)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab228178 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IHC-P		1/1. Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Pre-diluted / ready to use.
WB		Use at an assay dependent concentration.

Notes
IHC-P 1/1. Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Pre-diluted / ready to use.
WB Use at an assay dependent concentration.

Function

May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling.
Tissue specificity

Ubiquitous but not detectable in peripheral blood lymphocytes or granulocytes. Weakly expressed in resting monocytes. Expressed in dendritic cells derived from monocytes. Expressed in epithelial cells of sinonasal tissue. Expressed in extravillous trophoblast cells and Hofbauer cells of the first trimester placenta and term placenta.
Sequence similarities

Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 2 Ig-like V-type (immunoglobulin-like) domains.
Cellular localization

Membrane.
Target information above from: UniProt accession Q5ZPR3 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 80381 Human
- Entrez Gene: 102657 Mouse
- Entrez Gene: 315716 Rat
- Omim: 605715 Human
- SwissProt: Q5ZPR3 Human
- SwissProt: Q8VE98 Mouse
- SwissProt: Q7TPB4 Rat
- Unigene: 77873 Human
- Unigene: 5356 Mouse
- Unigene: 76129 Rat
see all
Alternative names
- 4Ig B7 H3 antibody
- 4Ig-B7-H3 antibody
- AU016588 antibody
- B7 H3 antibody
- B7 homolog 3 antibody
- B7-H3 antibody
- B7H3 antibody
- B7RP-2 antibody
- CD_antigen=CD276 antibody
- CD276 antibody
- CD276 antigen antibody
- CD276 molecule antibody
- CD276_HUMAN antibody
- Costimulatory molecule antibody
- Flags: Precursor antibody
- PSEC0249 antibody
- UNQ309/PRO352 antibody
see all

Western blot - Anti-CD276 antibody [SP206], prediluted (ab228178)

All lanes : Anti-CD276 antibody [SP206] (ab227670) at 1/400 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : CD276 knockout HeLa cell lysate
Lane 3 : LNCaP cell lysate
Lane 4 : Raji cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Observed band size: 90-110 kDa why is the actual band size different from the predicted?

This data was developed using the same antibody clone in a different buffer formulation (ab227670).

Lanes 1- 4: Merged signal (red and green). Green - ab227670 observed at 90-110 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab227670 was shown to react with CD276 antigen in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab261756 (knockout cell lysate ab257098) was used. Wild-type HeLa and CD276 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab227670 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 400 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178)

Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for CD276 with ab228178 in immunohistochemical analysis.
Western blot - Anti-CD276 antibody [SP206], prediluted (ab228178)

All lanes : Anti-CD276 antibody [SP206] (ab227670) at 1/1000 dilution

Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : CD276 knockout HEK293T cell lysate
Lane 3 : LNCaP cell lysate
Lane 4 : Raji cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Observed band size: 90-110 kDa why is the actual band size different from the predicted?

This data was developed using the same antibody clone in a different buffer formulation (ab227670).

Lanes 1-4: Merged signal (red and green). Green - ab227670 observed at 90-110 kDa. Red - loading control ab8245 observed at 36 kDa.

ab227670 Anti-CD276 antibody [SP206] was shown to specifically react with CD276 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266658 (knockout cell lysate ab257097) was used. Wild-type and CD276 knockout samples were subjected to SDS-PAGE. ab227670 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178)

Formalin-fixed, paraffin-embedded human stomach tissue stained for CD276 with ab228178 in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178)

Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for CD276 with ab228178 in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178)

Formalin-fixed, paraffin-embedded human breast tissue stained for CD276 with ab228178 in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178)

Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for CD276 with ab228178 in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178)

Formalin-fixed, paraffin-embedded human colon tissue stained for CD276 with ab228178 in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178)

Formalin-fixed, paraffin-embedded human lung SCC tissue stained for CD276 with ab228178 in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178)

Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for CD276 with ab228178 in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178)

Formalin-fixed, paraffin-embedded human lung tissue stained for CD276 with ab228178 in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178)

Formalin-fixed, paraffin-embedded human testis tissue stained for CD276 with ab228178 in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD276 with ab228178 in immunohistochemical analysis.
Anti-CD276 antibody [SP206], prediluted (ab228178)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

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Datasheet download

Download

Publishing research using ab228178? Please let us know so that we can cite the reference in this datasheet.

ab228178 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Purification notes

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

KO cell lines

KO cell lysates

KO cell pellets

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (0)

Customer reviews and Q&As