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  1. Link

    cd28-antibody-ab113358.pdf

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Immunology Adaptive Immunity B Cells CD
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Anti-CD28 antibody (ab113358)

  • Datasheet
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  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD28 antibody (ab113358)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD28 antibody (ab113358)
  • Western blot - Anti-CD28 antibody (ab113358)

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  • Datasheet
  • References
  • Protocols

Overview

  • Product name

    Anti-CD28 antibody
    See all CD28 primary antibodies
  • Description

    Goat polyclonal to CD28
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cynomolgus monkey
  • Immunogen

    Synthetic peptide:

    SQQLQVYSKTGFNCD

    , corresponding to internal sequence amino acids 73-87 of Human CD28 (NP_006130.1).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Human lymph node and tonsil lysates

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab113358 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • B Cells
    • CD
    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • NK Cell Lineage
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells

Associated products

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Recombinant Protein

    • Recombinant Human CD28 protein (His tag) (ab222975)

Applications

Our Abpromise guarantee covers the use of ab113358 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 25 kDa).
IHC-P Use at an assay dependent concentration.

Target

  • Function

    Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.
  • Tissue specificity

    Expressed in T-cells and plasma cells, but not in less mature B-cells.
  • Sequence similarities

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P10747 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 940 Human
    • Omim: 186760 Human
    • SwissProt: P10747 Human
    • Unigene: 591629 Human
    • Alternative names

      • CD 28 antibody
      • CD28 antibody
      • CD28 antigen antibody
      • CD28 molecule antibody
      • CD28_HUMAN antibody
      • MGC138290 antibody
      • T cell antigen CD28 antibody
      • T cell specific surface glycoprotein antibody
      • T cell specific surface glycoprotein CD28 antibody
      • T-cell-specific surface glycoprotein CD28 antibody
      • TP44 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD28 antibody (ab113358)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD28 antibody (ab113358)

      ab113358 (2µg/ml) staining of paraffin embedded Human Lymph Node. Microwaved antigen retrieval with citrate buffer Ph 6, HRP-staining.

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD28 antibody (ab113358)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD28 antibody (ab113358)

      ab113358 negative control showing staining of paraffin embedded Human Lymph Node, with no primary antibody.

    • Western blot - Anti-CD28 antibody (ab113358)
      Western blot - Anti-CD28 antibody (ab113358)
      Anti-CD28 antibody (ab113358) at 0.3 µg/ml + Human tonsil lysate (in RIPA buffer) at 35 µg

      Developed using the ECL technique.

      Predicted band size: 25 kDa
      Observed band size: 48 kDa
      why is the actual band size different from the predicted?



      Primary incubation was 1 hour.

      Note: The observed molecular weight corresponds to the glycosylated form.

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Datasheets and documents

    • Datasheet
  • References

    ab113358 has not yet been referenced specifically in any publications.

    Publishing research using ab113358? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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