Anti-CD28 antibody (ab113358)

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Product name

Anti-CD28 antibody
See all CD28 primary antibodies
Description

Goat polyclonal to CD28
Host species

Goat
Tested applications

Suitable for: WB, IHC-Pmore details
Species reactivity

Reacts with: Human
Predicted to work with: Cynomolgus monkey
Immunogen

Synthetic peptide:
SQQLQVYSKTGFNCD
, corresponding to internal sequence amino acids 73-87 of Human CD28 (NP_006130.1).

Run BLAST with Run BLAST with
Positive control
- Human lymph node and tonsil lysates

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
Purity

Immunogen affinity purified
Purification notes

ab113358 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality

Polyclonal
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab113358 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 25 kDa).
IHC-P		Use at an assay dependent concentration.

Function

Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.
Tissue specificity

Expressed in T-cells and plasma cells, but not in less mature B-cells.
Sequence similarities

Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localization

Membrane.
Target information above from: UniProt accession P10747 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 940 Human
- Omim: 186760 Human
- SwissProt: P10747 Human
- Unigene: 591629 Human
Alternative names
- CD 28 antibody
- CD28 antibody
- CD28 antigen antibody
- CD28 molecule antibody
- CD28_HUMAN antibody
- MGC138290 antibody
- T cell antigen CD28 antibody
- T cell specific surface glycoprotein antibody
- T cell specific surface glycoprotein CD28 antibody
- T-cell-specific surface glycoprotein CD28 antibody
- TP44 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD28 antibody (ab113358)

ab113358 (2µg/ml) staining of paraffin embedded Human Lymph Node. Microwaved antigen retrieval with citrate buffer Ph 6, HRP-staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD28 antibody (ab113358)

ab113358 negative control showing staining of paraffin embedded Human Lymph Node, with no primary antibody.
Western blot - Anti-CD28 antibody (ab113358)

Anti-CD28 antibody (ab113358) at 0.3 µg/ml + Human tonsil lysate (in RIPA buffer) at 35 µg

Developed using the ECL technique.

Predicted band size: 25 kDa
Observed band size: 48 kDa (why is the actual band size different from the predicted?)

Primary incubation was 1 hour.

Note: The observed molecular weight corresponds to the glycosylated form.

ab113358 has not yet been referenced specifically in any publications.

Publishing research using ab113358? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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