Anti-CD28 antibody (ab113358)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-CD28 antibody
See all CD28 primary antibodies -
Description
Goat polyclonal to CD28 -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cynomolgus monkey
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Immunogen
Synthetic peptide:
SQQLQVYSKTGFNCD
, corresponding to internal sequence amino acids 73-87 of Human CD28 (NP_006130.1). -
Positive control
- Human lymph node and tonsil lysates
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab113358 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab113358 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 25 kDa). | |
| IHC-P | Use at an assay dependent concentration. |
Target
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Function
Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. -
Tissue specificity
Expressed in T-cells and plasma cells, but not in less mature B-cells. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 940 Human
- Omim: 186760 Human
- SwissProt: P10747 Human
- Unigene: 591629 Human
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Alternative names
- CD 28 antibody
- CD28 antibody
- CD28 antigen antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD28 antibody (ab113358)ab113358 (2µg/ml) staining of paraffin embedded Human Lymph Node. Microwaved antigen retrieval with citrate buffer Ph 6, HRP-staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD28 antibody (ab113358)ab113358 negative control showing staining of paraffin embedded Human Lymph Node, with no primary antibody.
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Western blot - Anti-CD28 antibody (ab113358)Anti-CD28 antibody (ab113358) at 0.3 µg/ml + Human tonsil lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 25 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
Note: The observed molecular weight corresponds to the glycosylated form.
Datasheets and documents
References
ab113358 has not yet been referenced specifically in any publications.
