Overview

  • Product name

  • Description

    Rabbit polyclonal to CD28
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide within Mouse CD28 aa 20-45 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Sequence:

    NKILVKQSPLLVVDSNEVSLSCRYSY


    Database link: P31041

  • Positive control

    • Mouse splenocytes; rat colitis and mouse lung tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab203084 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500.

When using a fluorescent probe the recommended dilution is 1/50 – 1/200.

Flow Cyt 1/20 - 1/100.

Target

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin embedded rat colitis tissue labeling CD28 with ab203084 at 1/200 dilution followed by Goat Anti-Rabbit IgG, Cy3 conjugated at 1/200 dilution for 40 minutes at 37°C.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse lung tissue labeling CD28 with ab203084 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat colitis tissue labeling CD28 with ab203084 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.

  • Flow cytometric analysis of Mouse thymocytes labeling CD28 with ab203084 at 1/100 dilution for 30 minutes followed by incubation with a FITC conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

References

ab203084 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab203084.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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