Key features and details
- Rabbit polyclonal to CD28
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CD28 antibody
See all CD28 primary antibodies
DescriptionRabbit polyclonal to CD28
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow
Synthetic peptide within Human CD28 aa 1-50. The exact sequence is proprietary. NP_006130.1
Database link: P10747
- WB: HeLa, HEK-293T and Jurkat whole cell lysate. IP: Jurkat whole cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245327 was affinity purified using an epitope specific to CD28 immobilized on solid support.
Our Abpromise guarantee covers the use of ab245327 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 25 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionInvolved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.
Tissue specificityExpressed in T-cells and plasma cells, but not in less mature B-cells.
Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
- Information by UniProt
- CD 28 antibody
- CD28 antibody
- CD28 antigen antibody
CD28 was immunoprecipitated from 50 µg Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate using ab245327 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab245327 at 1 µg/ml.
Lane 1: ab245327 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in jurkat whole cell lysate.
Detection: Chemiluminescence with an exposure time of 3 minutes.
All lanes : Anti-CD28 antibody (ab245327) at 0.4 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 25 kDa
Exposure time: 3 minutes
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245327 has not yet been referenced specifically in any publications.