Overview

  • Product name

  • Description

    Rabbit polyclonal to CD28
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow
  • Immunogen

    Synthetic peptide within Human CD28 aa 1-50. The exact sequence is proprietary. NP_006130.1
    Database link: P10747

  • Positive control

    • WB: HeLa, HEK-293T and Jurkat whole cell lysate. IP: Jurkat whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab245327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 25 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

Images

  • CD28  was immunoprecipitated from 50 µg Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate using ab245327 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab245327 at 1 µg/ml.

    Lane 1: ab245327 IP in Jurkat whole cell lysate.
    Lane 2: Control IgG IP in jurkat whole cell lysate.

    Detection: Chemiluminescence with an exposure time of 3 minutes.

     

     

  • All lanes : Anti-CD28 antibody (ab245327) at 0.4 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 25 kDa


    Exposure time: 3 minutes

References

ab245327 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab245327.
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